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Genetic analysis of Agrobacterium tumefaciens susceptibility in Brassica oleracea

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Abstract

The genetic control and heritability of Agrobacterium tumefaciens susceptibility was investigated using a doubled haploid (DH) mapping population of Brassica oleracea and the associated RFLP map. Preliminary studies were carried out by analysis of an 8×8 diallel, for which the parental lines were selected to include a range of susceptibilities to A. tumefaciens. The variation observed within the diallel was attributed to both additive and dominant gene effects, with additive gene effects being more important. A broad sense heritability value of 0.95 suggested that 95% of the observed variation was due to genetic effects, with just 5% attributed to non-genetic or environmental effects. A high narrow-sense heritibility value of 0.79 suggested that 79% of this trait was controlled by additive gene effects and, therefore, the potential to introduce this trait into breeding material is high. Fifty-nine DH lines from the mapping population were screened for susceptibility towards A. tumefaciens. Variation in susceptibility was observed across the population. The results of the DH screen were entered into the mapping programme MAPQTL and a highly significant quantitative trait loci (QTL) associated with susceptibility to A. tumefaciens was identified on linkage group 09. The use of substitution lines covering this region confirmed the location of this QTL. This work shows that susceptibility to A. tumefaciens is a heritable trait, and the transfer of susceptibility into resistant lines is demonstrated. These findings may help to overcome genotype restrictions to genetic transformation.

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Acknowledgements

The authors would like to thank Drs. D. Laurie, M. Boulton and M. Perry for their useful comments on this manuscript. The authors also thank the Department of environment, food and rural affairs (Defra) and the Biotechnology and Biological Science Research Council (BBSRC) for their support. This work was funded by Defra project HH0909SFV as part of an investigation of the genetic basis of transformation in horticultural brassicas.

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  1. John Innes Centre, Norwich, NR4 7UH, UK

    P. A. C. Sparrow, T. M. Townsend, A. E. Arthur, P. J. Dale & J. A. Irwin

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  1. P. A. C. Sparrow
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  2. T. M. Townsend
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  3. A. E. Arthur
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  4. P. J. Dale
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  5. J. A. Irwin
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Correspondence to P. A. C. Sparrow.

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Communicated by G. Wenzel

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Sparrow, P.A.C., Townsend, T.M., Arthur, A.E. et al. Genetic analysis of Agrobacterium tumefaciens susceptibility in Brassica oleracea . Theor Appl Genet 108, 644–650 (2004). https://doi.org/10.1007/s00122-003-1473-z

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  • DOI: https://doi.org/10.1007/s00122-003-1473-z

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