Abstract
The aim of this work was to evaluate the components of the genetic mean values and variances of the in vitro culture response in a cross between cultivar ‘Caimanta’ of the cultivated L. esculentum as the high regeneration capacity genotype and accession LA722 of the exotic L. pimpinellifolium as the low regeneration capacity genotype. The callus percentage (C, number of cultures that only produced callus × 100/total number of explants), the regeneration percentage (R, number of cultures that differentiated into completely developed shoots and/or primordial × 100/total number of explants) and the productivity rate (PR, total number of completely developed shoots/total number of cultures) were evaluated 30 days after culture initiation in the parents, F1, F2 and backcrosses. The in vitro culture response was controlled by gene action of complete dominance of the cultivated genome over the exotic one. Genetic interaction was not detected in these sets of data. The narrow sense heritability (h2) values were 0 for C and R, and 0.62 for PR. In consequence, genetic variability was mostly non-additive for both callus and regeneration percentages, while it was mostly additive for the productivity rate.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- C:
-
Callus percentage
- M:
-
Means
- h2 :
-
Narrow sense heritability
- PR:
-
Productivity rate
- R:
-
Regeneration percentage
References
Abe T, Futsuhara Y (1991) Diallel analysis of callus growth and plant regeneration in rice seed-callus. Jpan J Genet 66:129–140
Compton ME (1994) Statistical methods suitable for analysis of plant tissue culture data. Plant Cell Tissue Organ Cult 37:217–242
Fambrini M, Fisichella M, Pugliesi C (2001) Enhanced morphogenetic potencial from in vitro regenerated plants of genus Helianthus: an overview. Recent Res Dev Plant Biol 1:35–54
Faria R, Illg RD (1996) Inheritance of in vitro plant regeneration ability in the tomato. Genet Mol Biol 19:113–116
Faria R, Destro D, Bespalhok Filho JC, Illg RD (2002) Introgression of in vitro regeneration capability of Lycopersicon pimpinellifolium Mill. into recalcitrant tomato cultivars. Euphytica 124:59–63
Flores Berrios EF, Gentzbittel L, Alibert G, Griveau Y, Bervillé A, Sarrafi A (1999) Genetic control of in vitro organogenesis in recombinant inbred lines of sunflower (Helianthus annuus L.). Plant Breed 118:359–361
Flores Berrios E, Gentzbittel L, Kayyal H, Alibert G, Sarrafi A (2000) AFLP mapping of QTLs for in vitro organogenesis traits using recombinant inbred lines in sunflower Helianthus annus L. Theor Appl Genet 101:1299–1306
Frankerberger EA, Hasegawa PM, Tigchelaar EC (1981) Diallel analysis of shoot-forming capacity among selected tomato genotypes. Z Pflanzenphysiol 102:233–242
Henry Y, Vain P, De Buyser J (1994) Genetic analysis of in vitro plant tissue culture responses and regeneration capacities. Euphytica 79:45–58
Kearsey MJ, Pooni HS (1996) The genetical analysis of quantitative traits. Chapman and Hall, London, UK
Koornneef M, Hanhart CJ, Martinelli L (1987) A genetic analysis of cell culture traits in tomato. Theor Appl Genet 74:633–641
Kuroda S, Kato H, Ikeda R (1998) Heterosis and combining ability for callus growth rate in rice. Crop Sci 38:933–936
Lipucci Di Paolo M, Collina Grenci F, Scala A (1983) Shoot forming ability of Lycopersicon esculentum Mill. varieties by in vitro cultured cotyledons. Acta Hort 131:111–116
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Nestares G, Zorzoli R, Mroginski LA, Picardi LA (1998) Cytoplasm effects on the regeneration ability of sunflower. Plant Breed 117:188–190
Nesticky M, Novak FJ, Piovarci A, Dolezelová M (1983) Genetic analysis of callus growth of maize (Zea mays. L.) in vitro. Z Pflanzenzücht 91:322–328
Plastira VA, Perdikaris A (1997) Effect of genotype and explant type in regeneration frequency of tomato in vitro. Acta Hort 447:231–234
Pratta G, Zorzoli R, Picardi LA (1997) Intra and interspecific variability of in vitro culture response in Lycopersicon tomatoes. Braz J Genet 20:75–78
Pratta G, Cánepa LN, Zorzoli R, Picardi LA (2003) Diallel analysis of in vitro culture traits in the genus Lycopersicon. HortScience 38:110–112
Pratta G, Zorzoli R, Picardi LA, Valle EM (2006) Variability for the in vitro culture response in a set of tomato genotypes. Biol Plant 50:4214–424
Shapiro SS, Wilks MB (1965) An analysis of variance test for normality (complete samples). Biometrika 52: 591–611
Tal M, Dehan K, Heiken H (1977) Morphogenetic potential of cultured leaf sections of cultivated and wild species of tomato. Ann Bot 41:937–941
Author information
Authors and Affiliations
Corresponding author
Additional information
This manuscript is part of the Thesis Dissertation of EMB for obtaning the degree of Licenciate in Biotechnology at the Universidad Nacional de Rosario.
Rights and permissions
About this article
Cite this article
Marchionni Basté, E., Pratta, G.R. & Zorzoli, R. Genetic analysis of the in vitro culture response in tomato. Plant Cell Tiss Organ Cult 88, 233–239 (2007). https://doi.org/10.1007/s11240-006-9195-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11240-006-9195-y