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Overexpression of a heat shock protein (ThHSP18.3) from Tamarix hispida confers stress tolerance to yeast

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Abstract

It is well known that plant heat shock proteins (HSPs) play important roles both in response to adverse environmental conditions and in various developmental processes. However, among plant HSPs, the functions of tree plant HSPs are poorly characterized. To improve our understanding of tree HSPs, we cloned and characterized an HSP gene (ThHSP18.3) from Tamarix hispida. Sequence alignment reveals that ThHSP18.3 belongs to the class I small heat shock protein family. A transient expression assay showed that ThHSP18.3 protein was targeted to the cell nucleus. Treatment of Tamarix hispida with cold and heat shock highly induced ThHSP18.3 expression in all studied leaves, roots and stems, whereas, treatment of T. hispida with NaCl, NaHCO3, and PEG induced ThHSP18.3 expression in leaves and decreased its expression in roots and stems. Further, to study the role of ThHSP18.3 in stress tolerance under different stress conditions, we cloned ThHSP18.3 into the pYES2 vector, transformed and expressed the vector in yeast Saccharomyces cerevisiae. Yeast cells transformed with an empty pYES2 vector were employed as a control. Compared to the control, yeast cells expressing ThHSP18.3 showed greater tolerance to salt, drought, heavy metals, and both low and high temperatures, indicating that ThHSP18.3 confers tolerance to these stress conditions. These results suggested that ThHSP18.3 is involved in tolerance to a variety of stress conditions in T. hispida.

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Acknowledgments

This work has been supported by Genetically modified organisms breeding major projects (no. 2009ZX08009-098B), the Fundamental Research Funds for the Central Universities (no. DL12CA03) and the National Natural Science Foundation of China (no. 31000312).

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Authors and Affiliations

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, People’s Republic of China

    Caiqiu Gao, Bo Jiang, Yucheng Wang, Guifeng Liu & Chuanping Yang

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  1. Caiqiu Gao
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  2. Bo Jiang
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  3. Yucheng Wang
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  5. Chuanping Yang
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Correspondence to Chuanping Yang.

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Caiqiu Gao and Bo Jiang have the same contribution to this paper.

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Gao, C., Jiang, B., Wang, Y. et al. Overexpression of a heat shock protein (ThHSP18.3) from Tamarix hispida confers stress tolerance to yeast. Mol Biol Rep 39, 4889–4897 (2012). https://doi.org/10.1007/s11033-011-1284-2

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