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Expression profiles of hot pepper (capsicum annuum) genes under cold stress conditions

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Abstract

In an attempt to determine a cold defense mechanism in plants, we have attempted to characterize changes occurringin the expression of cold-regulated transcript levels in the hot pepper (Capsicum annuum), using cDNA microarray analysis, combined with Northern blot analysis. After analysing a 3.1 K hot pepper cDNA microarray, we isolated a total of 317 cold inducible genes. We selected 42 genes which were up-regulated and three genes which were down-regulated due to cold treatment, for further analysis. Among the 45 genes which appeared to be upregulated by cold, 19 genes appeared to be simultaneously regulated by salt stress. Among the up-regulated cold-stress genes, we identified a variety of transcription factors, including: a family of 4 ethylene-responsive element binding protein (EREBP, designated CaEREBP-C1 to C4) genes, a bZIP protein (CaBZ1), RVA1, Ring domain protein, HSF1, and the WRKY (CaWRKY1) protein. As mentioned earlier, several genes appeared to be induced not only by cold stress, but also simultaneously by salt stress. These genes included: CaEREBP-C3, CaBZ1, putativetrans-activator factor, NtPRp27, malate dehydrogenase, putative auxin-repressed protein, protein phosphatase (CaTPP1), SAR8.2 protein precursor, late-embryogenesis abundant protein 5 (LEA5), DNAJ protein homologue, xyloglucanendo-l,4-Β-D-gucanase precursor, PR10, and the putative non-specific lipid transfer protein StnsLTP.

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Authors and Affiliations

  1. Division of Applied Biological Sciences, Sunmoon University, 336-708, Asan, Korea

    Eul-Won Hwang, Kyung-A Kim, Soo-Chul Park, Mi-Jeong Jeong, Myung-Ok Byun & Hawk-Bin Kwon

  2. Abiotic and Biotic Stress Team, National Institute of Agricultural Biotechnology, 441-707, Suwon, Korea

    Soo-Chul Park, Mi-Jeong Jeong & Myung-Ok Byun

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  1. Eul-Won Hwang
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  2. Kyung-A Kim
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  3. Soo-Chul Park
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  4. Mi-Jeong Jeong
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  6. Hawk-Bin Kwon
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Correspondence to Hawk-Bin Kwon.

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Hwang, EW., Kim, KA., Park, SC. et al. Expression profiles of hot pepper (capsicum annuum) genes under cold stress conditions. J. Biosci. 30, 657–667 (2005). https://doi.org/10.1007/BF02703566

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