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Genome-wide identification of QTL conferring high-temperature adult-plant (HTAP) resistance to stripe rust (Puccinia striiformis f. sp. tritici) in wheat

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Abstract

High-temperature adult-plant (HTAP) resistance to stripe rust (caused by Puccinia striiformis f. sp. tritici) is a durable type of resistance in wheat (Triticum aestivum L.). This study identified quantitative trait loci (QTL) conferring HTAP resistance to stripe rust in a population consisting of 169 F8:10 recombinant inbred lines (RILs) derived from a cross between a susceptible cultivar Rio Blanco and a resistant germplasm IDO444. HTAP resistance was evaluated for both disease severity and infection type under natural infection over two years at two locations. The genetic linkage maps had an average density of 6.7 cM per marker across the genome and were constructed using 484 markers including 96 wheat microsatellite (SSR), 632 Diversity Arrays Technology (DArT) polymorphisms, two sequence-tagged-site (STS) from semi-dwarf genes Rht1 and Rht2, and two markers for low molecular-weight glutenin gene subunits. QTL analysis detected a total of eight QTL significantly associated with HTAP resistance to stripe rust with two on chromosome 2B, two on 3B and one on each of 1A, 4A, 4B and 5B. QTL on chromosomes 2B and 4A were the major loci derived from IDO444 and explained up to 47 and 42% of the phenotypic variation for disease severity and infection type, respectively. The remaining five QTL accounted for 7–10% of the trait variation. Of these minor QTL, the resistant alleles at the two QTL QYrrb.ui-3B.1 and QYrrb.ui-4B derived from Rio Blanco and reduced infection type only, while the resistant alleles at the other three QTL, QYrid.ui-1A, QYrid.ui-3B.2 and QYrid.ui-5B, all derived from IDO444 and reduced either infection type or disease severity. Markers linked to 2B and 4A QTL should be useful for selection of HTAP resistance to stripe rust.

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Acknowledgments

This project was supported by the National Research Initiative Competitive Grants 2006-55606-16629, the USDA National Institute of Food and Agriculture Triticeae-CAP 2011-68002-30029, and the Idaho Wheat Commission Grant. The authors sincerely thank Dr. Deven R. See, Dr. Paul St Amand, Dr. Gina Brown-Guediera, Dr. Daryl Klindworth, Mr. Weidong Zhao, Mrs. Karen Peterson and Mr. Junli Zhang for technical assistance in genotyping SSR markers.

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Author notes

  1. Chenggen Chu

    Present address: Heartland Plant Innovations Inc., 217, Southwind Place, Manhattan, KS, 66503, USA

Authors and Affiliations

  1. Department of Plant Soil and Entomological Sciences, University of Idaho, 1691 S 2700 W, Aberdeen, ID, 83210, USA

    Jianli Chen

  2. Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA

    Chenggen Chu

  3. USDA-ARS, Soft Wheat Quality Lab, Wooster, OH, 44691, USA

    Edward J. Souza

  4. Department of Plant Pathology, Ohio State University, Wooster, OH, 44691, USA

    Mary J. Guttieri

  5. USDA-ARS, Washington State University, Pullman, WA, 99164, USA

    Xianming Chen

  6. USDA-ARS, Northern Crop Science Laboratory, Fargo, ND, 58102, USA

    Steven Xu

  7. Department of Plants, Soils and Climate, Utah State University, Logan, UT, 84322, USA

    David Hole

  8. Department of Plant Soil and Entomological Sciences, University of Idaho, Moscow, ID, 83844, USA

    Robert Zemetra

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Correspondence to Jianli Chen.

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11032_2011_9590_MOESM2_ESM.pdf

Fig. 1 S1 Genetic linkage maps constructed for the Rio Blanco × IDO444 RIL population. The positions of marker loci are shown to the right of the linkage groups and the centiMorgan (cM) distances between loci are shown along the left. LMW: low-molecular-weight glutenin gene subunit; Rht: reduced height genes (PDF 220 kb)

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Chen, J., Chu, C., Souza, E.J. et al. Genome-wide identification of QTL conferring high-temperature adult-plant (HTAP) resistance to stripe rust (Puccinia striiformis f. sp. tritici) in wheat. Mol Breeding 29, 791–800 (2012). https://doi.org/10.1007/s11032-011-9590-x

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