Abstract
The yeast Yarrowia lipolytica is an industrially important microorganism with distinctive physiological and metabolic characteristics. A variety of external factors (e.g., pH, temperature, and nutrient availability) influences the behavior of the yeast and may act as stress conditions which the cells must withstand and adapt. In this mini review, the impacts of environmental factors on the morphology and metabolite production by Y. lipolytica are summarized. In this regard, detailed insights into the effectors involved in the dimorphic transition of Y. lipolytica, the cultivation conditions employed, as well as the methods applied for the morphological characterization are highlighted. Concerning the metabolism products, a special focus is addressed on lipid and citric acid metabolites which have attracted significant attention in recent years. The dependence of lipid and citric acid productivity on key process parameters, such as media composition and physico-chemical variables, is thoroughly discussed. This review attempts to provide a recent update on the topic and will serve as a meaningful resource for researchers working in the field.
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Funding
Financial support for this study was provided by Airbus, Agence Nationale de la Recherche (ANR) and Commissariat aux Investissements d’Avenir via the project ProBio3 “Biocatalytic production of lipidic bioproducts from renewable resources and industrial by-products: BioJet Fuel Application” (ref. ANR-11-BTBT-0003).
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Timoumi, A., Guillouet, S.E., Molina-Jouve, C. et al. Impacts of environmental conditions on product formation and morphology of Yarrowia lipolytica. Appl Microbiol Biotechnol 102, 3831–3848 (2018). https://doi.org/10.1007/s00253-018-8870-3
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DOI: https://doi.org/10.1007/s00253-018-8870-3