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Dimorphism and hydrocarbon metabolism in Yarrowia lipolytica var. indica

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Abstract

Yarrowia lipolytica is able to metabolize high Mr hydrophobic natural compounds such as fatty acids and hydrocarbons. Characteristically, strains of Y. lipolytica can grow as populations with variable proportions of yeast and filamentous forms. In the present study, we describe the dimorphic characteristics of a variant designated as Y. lipolytica var. indica isolated from petroleum contaminated sea water and the effect of cell morphology on hydrocarbon metabolism. The variant behaved as a yeast monomorphic strain, under conditions at which terrestrial Y. lipolytica strain W29 and its derived strains, grow as almost uniform populations of mycelial cells. Using organic nitrogen sources and N-acetylglucosamine as carbon source, var. indica was able to form mycelial cells, the proportion of which increased when incubated under semi-anaerobic conditions. The cell surface characteristics of var. indica and W29 were found to be different with respect to contact angle and percent hydrophobicity. For instance, percent hydrophobicity of var. indica was 89.93 ± 1.95 while that of W29 was 70.78 ± 1.1. Furthermore, while all tested strains metabolize hydrocarbons, only var. indica was able to use it as a carbon source. Yeast cells of var. indica metabolized hexadecane with higher efficiency than the mycelial form, whereas the mycelial form of the terrestrial strain metabolized the hydrocarbon more efficiently, as occurred with the mycelial monomorphic mutant AC11, compared to the yeast monomorphic mutant AC1.

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Acknowledgments

Authors are grateful to Department of Science and Technology, New Delhi and CONACYT, México, for the financial support to the Indo-Mexico visit exchange programme. ASP thanks Council of Scientific and Industrial Research, India for research fellowship. SVK thanks University Grants Commission (UGC) for Teacher’s Fellowship. Authors are thankful to Dr. A. Sen, National Chemical Laboratory, Pune for the GC analysis.

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Authors and Affiliations

  1. Biochemical Sciences Division, National Chemical Laboratory, Pune, 411008, India

    A. S. Palande, S. V. Kulkarni & M. V. Deshpande

  2. Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Guanajuato, Mexico

    C. León-Ramirez & J. Ruiz-Herrera

  3. Centro de Investigación en Nutrición y Salud Pública, Universidad Autónoma de Nuevo León, Monterrey, NL, Mexico

    E. Campos-Góngora

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  1. A. S. Palande
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  2. S. V. Kulkarni
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  3. C. León-Ramirez
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  4. E. Campos-Góngora
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  5. J. Ruiz-Herrera
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  6. M. V. Deshpande
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Correspondence to M. V. Deshpande.

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Communicated by Erko Stackebrandt.

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Palande, A.S., Kulkarni, S.V., León-Ramirez, C. et al. Dimorphism and hydrocarbon metabolism in Yarrowia lipolytica var. indica . Arch Microbiol 196, 545–556 (2014). https://doi.org/10.1007/s00203-014-0990-2

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  • DOI: https://doi.org/10.1007/s00203-014-0990-2

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