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Molecular cytogenetic identification of a new wheat-Thinopyrum substitution line with stripe rust resistance

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Abstract

A new wheat-Thinopyrum substitution line AS1677, developed from a cross between wheat line ML-13 and wheat-Thinopyrum intermedium ssp. trichophorum partial amphiploid TE-3, was characterized by fluorescence in situ hybridization (FISH), sequential Giemsa-C banding, genomic in situ hybridization (GISH), seed storage protein electrophoresis, molecular marker analysis and disease resistance screening. Sequential Giemsa-C banding and GISH using Pseudoroegneria spicata genomic DNA as probe indicated that a pair of St-chromosomes with strong terminal bands were introduced into AS1677. FISH using pTa71 as a probe gave strong hybridization signals at the nuclear organization region and in the distal region of the short arms of the St chromosome. Moreover, FISH using the repetitive sequence pAs1 revealed that a pair of wheat 1D chromosomes was absent in accession AS1677. Seed storage proteins separated by acid polyacrylamide gel electrophoresis (APAGE) and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that AS1677 lacked the gliadin and glutenin bands encoded by Gli-D1 and Glu-D1, further confirming the absence of chromosome 1D. The introduced St chromosome pair belonging to homoeologous group 1 was identified by newly produced genome specific markers. AS1677 is a new 1St (1D) substitution line. When inoculated with stripe rust and powdery mildew isolates, AS1677 expressed stripe rust resistance possibly derived from its Thinopyrum parent. AS1677 can be used as a donor source for introducing novel disease resistance genes to wheat in breeding programs aided by molecular and cytogenetic markers.

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Acknowledgments

We thank the National Natural Science Foundation of China (no. 30170502, 30871518), Chinese Ministry of Education (NCET-06-0810), and Young Scholars Foundation from the Science and Technology Committee of Sichuan (2008-31-371) for financial support. We particularly thank Dr. W. Jon Raupp, Kansas State University, Manhattan, Kansas, for critical review of the manuscript.

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Authors and Affiliations

  1. School of Life Science and Technology, University of Electronic Science and Technology of China, 610054, Chengdu, China

    Li-Jun Hu, Guang-Rong Li, Zi-Xian Zeng, Cheng Liu, Jian-Ping Zhou & Zu-Jun Yang

  2. Crop Genetic Institute of Shanxi Academy of Agricultural Sciences, 030031, Taiyuan, China

    Zhi-Jian Chang

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  1. Li-Jun Hu
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  2. Guang-Rong Li
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Correspondence to Zu-Jun Yang.

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Hu, LJ., Li, GR., Zeng, ZX. et al. Molecular cytogenetic identification of a new wheat-Thinopyrum substitution line with stripe rust resistance. Euphytica 177, 169–177 (2011). https://doi.org/10.1007/s10681-010-0216-x

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