Summary
The chromosomal assignment of 64 PCR-amplified microsatellite loci and 29 additional fragments amplified by the same primer pairs is described for bread wheat (Triticum aestivum). The distribution over the different chromosomes and chromosome arms appears to be random. The highest proportion of microsatellite loci is found on the B genome, followed by the A and D genome. About half of the primer pairs amplified unique fragments, while the other half amplified additional fragments. 25% of the primer pairs, mostly designed to clones of a PstI-library, amplify fragments on homoeologous chromosomes. In some cases, more than one fragment on a single chromosome or fragments on non-homoeologous chromosomes occurred. The use of an automated DNA sequencer accounts for the accurate resolution of multiple fragments and enables to differentiate between fragments, amplified by a single primer pair, with size differences as small as two base pairs.
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References
Anderson, J.A., Y. Ogihara, M.E. Sorrells & S.D. Tanksley, 1992. Development of a chromosomal arm map for wheat based on RFLP markers. Theor. Appl. Genet. 83: 1035–1043.
Akkaya, M.S., A.A. Bhagwat & P.B. Cregan, 1992. Length polymorphisms of simple sequence repeat DNA in soybean. Genetics 132: 1131–1139.
Bell, C.J. & J.R. Ecker, 1994. Assignment of 30 microsatellite loci to the linkage map of Arabidopsis. Genomics 19: 137–144.
Bishop, M.D., S.M. Kappes, J.W. Keele, R.T. Stone, S.L.F. Sunden, G.A. Hawkins, S.S. Toldo, R. Fries, M.D. Grosz, J. Yoo & C.W. Beattie, 1994. A genetic linkage map for cattle. Genetics 136: 619–639.
Copeland, N.G., N.A. Jenkins, D.J. Gilbert, J.T. Eppig, L.J. Maltais, J.C. Miller, W.F. Dietrich, A. Weavr, S.E. Lincoln, R.G. Steen, L.D. Stein, J.H. Nadeau & E.S. Lander, 1993. A genetic linkage map of the mouse: Current applications and further prospects. Science 262: 57–66.
Devos, K.M., M.D. Atkinson, C.N. Chinoy, C. Liu & M.D. Gale, 1992. RFLP-based genetic map of the homoeologous group 3 chromosomes of wheat and rye. Theor. Appl. Genet. 82: 931–939.
Gale, M.D., S. Chao & P.J. Sharp, 1990. RFLP mapping in wheat — progress and problems. p. 353–363. In: J.P. Gustafson (Ed). Gene Manipulation in Plant Improvement II. Plenum Press, New York.
Gill, K.S., E.L. Lubbers, B.S. Gill, W.J. Raupp & T.S. Cox, 1991. A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome 34: 362–374.
Hart, G.E. & M.D. Gale, 1990. Biochemical/molecular loci of hexaploid wheat (Triticum aestivum 2n=42 genomes AABBDD). p. 6.28–6.38. In: S.J. O'Brien (Ed). Genetic Maps, Vol. 5. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Liu, Y.G. & K. Tsunewaki, 1991. Restriction fragment length polymorphism (RFLP) analysis in wheat. II. Linkage maps of the RFLP sites in common wheat. Jpn. J. Genet. 66: 617–633.
Liu, C.J., M.D. Atkinson, C.N. Chinoy, K.M. Devos & M.D. Gale, 1992. Nonhomoeologous translocations between group 4, 5 and 7 chromosomes within wheat and rye. Theor. Appl. Genet. 83: 305–312.
Maroof, M.A.S., R.M. Biyashey, G.P. Yang, Q. Zhang & R.W. Allard, 1994. Extraordinarily polymorphic microsatellite DNA in barley: Species diversity, chromosomal locations, and population dynamics. Proc. Natl. Acad. Sci. 91: 5466–5470.
McIntosh, R.A., 1988. A catalogue of gene symbols for wheat. p. 1225–1323. In: T.E. Miller & R.M.D. Koebner (Eds). Proc. 7th Int. Wheat Genet. Symp., Cambridge, England. Bath Press, Bath, Avon.
Morgante, M. & A.M. Olivieri, 1993. PCR-amplified microsatellites as markers in plant genetics. Plant J. 3: 175–182.
Morris, R. & T.E. Miller, 1988. Workshop I. Genome allocation of chromosome 4A and 4B. p. 1211. In: T.E. Miller & R.M.D. Koebner (Eds). Proc. 7th Int. Wheat Genet. Symp., Cambridge, England. Bath Press, Bath, Avon.
Naranjo, T., A. Roca, P.G. Giocoecha & R. Giraldez, 1987. Arm homoeology of wheat and rye chromosomes. Genome 29: 873–882.
Röder, M.S., J. Plaschke, S.U. König, A. Börner, M.S. Sorrells, S.D. Tanksley & M.W. Ganal, 1995. Abundance, variability and chromosomal location of microsatellites in wheat. Mol. Gen. Genet. 246: 327–333.
Rohrer, G.A., L.J. Alexander, J.W. Keele, T.P. Smith & C.W. Beattie, 1994. A microsatellite linkage map of the porcine genome. Genetics 136: 231–245.
Sears, E.R., 1954. The aneuploids of common wheat. Mo. Agric. Exp. Stn. Res. Bull. 572: 1–58.
Sears, E.R., 1966. Nullisomic-tetrasomic combinations in hexaploid wheat. p. 29–45. In: R. Riley & K.R. Lewis (Eds). Chromosome Manipulations and Plant Genetics. Oliver and Boyd, London.
Sears, E.R. & L.M.S. Sears, 1978. The telocentric chromosomes of common wheat. p. 389–407. In: S. Ramanujam (Ed). Proc. 5th Int. Wheat Genet. Symp. Indian Soc. Plant Breed. Genet., Delhi.
Weissenbach, J., G. Gyapay, C. Dib, A. Vignal, J. Morissette, P. Millasseau, G. Vaysseix & M. Lathrop, 1992. A second-generation linkage map of the human genome. Nature 359: 794–801.
Wu, K.S. & S.D. Tanksley, 1993. Abundance, polymorphism and genetic mapping of microsatellites in rice. Mol. Gen. Genet. 241: 225–235.
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Plaschke, J., Börner, A., Wendehake, K. et al. The use of wheat aneuploids for the chromosomal assignment of microsatellite loci. Euphytica 89, 33–40 (1996). https://doi.org/10.1007/BF00015716
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DOI: https://doi.org/10.1007/BF00015716