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Genetic linkage map construction and QTL mapping of cadmium accumulation in radish (Raphanus sativus L.)

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Abstract

Cadmium (Cd) is a widespread soil pollutant and poses a significant threat to human health via the food chain. Large phenotypic variations in Cd concentration of radish roots and shoots have been observed. However, the genetic and molecular mechanisms of Cd accumulation in radish remain to be elucidated. In this study, a genetic linkage map was constructed using an F2 mapping population derived from a cross between a high Cd-accumulating cultivar NAU-Dysx and a low Cd-accumulating cultivar NAU-Yh. The linkage map consisted of 523 SRAP, RAPD, SSR, ISSR, RAMP, and RGA markers and had a total length of 1,678.2 cM with a mean distance of 3.4 cM between two markers. All mapped markers distributed on nine linkage groups (LGs) having sizes between 134.7 and 236.8 cM. Four quantitative trait loci (QTLs) for root Cd accumulation were mapped on LGs 1, 4, 6, and 9, which accounted for 9.86 to 48.64 % of all phenotypic variance. Two QTLs associated with shoot Cd accumulation were detected on LG1 and 3, which accounted for 17.08 and 29.53 % of phenotypic variance, respectively. A major-effect QTL, qRCd9 (QTL for root Cd accumulation on LG9), was identified on LG 9 flanked by NAUrp011_754 and EM5me6_286 markers with a high LOD value of 23.6, which accounted for 48.64 % of the total phenotypic variance in Cd accumulation of F2 lines. The results indicated that qRCd9 is a novel QTL responsible for controlling root Cd accumulation in radish, and the identification of specific molecular markers tightly linked to the major QTL could be further applied for marker-assisted selection (MAS) in low-Cd content radish breeding program.

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Acknowledgments

This work was in part supported by grants from the Program for NCET, MOE (NCET-10-047), the National Key Technologies R & D Program of China (2009BADB8B03), Key Technology R & D Program of Jiangsu Province (BE2010328) and the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD). We thank Dr. J. Hu from USDA-ARS for his critical review and helpful comments during the preparation of this manuscript.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Liang Xu, Liangju Wang, Yiqin Gong, Yan Wang, Tiancai Wen & Liwang Liu

  2. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA

    Wenhao Dai & Xianwen Zhu

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  1. Liang Xu
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  2. Liangju Wang
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  3. Yiqin Gong
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  4. Wenhao Dai
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  5. Yan Wang
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  7. Tiancai Wen
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  8. Liwang Liu
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Correspondence to Liwang Liu.

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Communicated by I. Rajcan.

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Xu, L., Wang, L., Gong, Y. et al. Genetic linkage map construction and QTL mapping of cadmium accumulation in radish (Raphanus sativus L.). Theor Appl Genet 125, 659–670 (2012). https://doi.org/10.1007/s00122-012-1858-y

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  • DOI: https://doi.org/10.1007/s00122-012-1858-y

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