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Identification of QTLs for morphological traits influencing waterlogging tolerance in perennial ryegrass (Lolium perenne L.)

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Abstract

Perennial ryegrass is a globally cultivated obligate outbreeding diploid species (2n = 2x = 14) which is subjected to periods of waterlogging stress due to flood irrigation during winter and the lead-up to summer. Reduction of oxygen supply to root systems due to waterlogging produces consequent deleterious effects on plant performance. Framework genetic maps for a large-scale genetic mapping family [F 1(NA x  × AU6)] were constructed containing 91 simple sequence repeat and 24 single nucleotide polymorphism genetic markers. Genetic trait dissection using both control and waterlogging treatments was performed in the glasshouse, a total of 143 maximally recombinant genotypes being selected from the overall sib-ship and replicated threefold in the trial. Analysis was performed for nine quantitative morphological traits measured 8 weeks after stress treatments were applied. A total of 37 quantitative trait loci (QTLs) were identified; 19 on the NA x parental genetic map, and 18 on the AU6 parental genetic map. Regions of particular interest were identified on linkage groups (LGs) 4 and 3 of the respective maps, which have been targeted for further analysis by selection of critical recombinants. This first study of genetic control of waterlogging tolerance in ryegrasses has important implications for breeding improvement of abiotic stress adaptation.

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Acknowledgments

This work was supported by funding from the Victorian Department of Primary Industries, Dairy Australia Ltd., the Geoffrey Gardiner Dairy Foundation, Meat and Livestock Australia Ltd. and the Molecular Plant Breeding Cooperative Research Centre (MPB CRC). The authors thank Prof. German Spangenberg for careful critical reading of the manuscript.

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Authors and Affiliations

  1. Department of Primary Industries, Biosciences Research Division, Victorian AgriBiosciences Centre, La Trobe Research and Development Park, Bundoora, VIC, 3083, Australia

    Allison Pearson, Noel O. I. Cogan, Rebecca C. Baillie, Melanie L. Hand & John W. Forster

  2. Department of Primary Industries, Biosciences Research Division, Hamilton Centre, Hamilton, VIC, 3300, Australia

    Champa K. Bandaranayake, Stacey Erb, Junping Wang, Gavin A. Kearney & Kevin F. Smith

  3. Molecular Plant Breeding and Dairy Futures Cooperative Research Centres, Bundoora, VIC, Australia

    Allison Pearson, Noel O. I. Cogan, Rebecca C. Baillie, Melanie L. Hand, Champa K. Bandaranayake, Stacey Erb, Junping Wang, Kevin F. Smith & John W. Forster

  4. Department of Botany, La Trobe University, Bundoora, VIC, 3086, Australia

    Allison Pearson, Anthony R. Gendall, Kevin F. Smith & John W. Forster

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  1. Allison Pearson
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Correspondence to John W. Forster.

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Communicated by P. Langridge.

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122_2010_1473_MOESM1_ESM.ppt

S1 Frequency distributions of the 569 individuals used for the framework genetic map construction (blue), depicting the number of recombination events occurring in the large-scale mapping population. Purple bars represent the 143 individuals that were chosen using MapPop and the number or recombination events that observed (PPT 82 kb)

122_2010_1473_MOESM2_ESM.doc

S2 Correlation coefficients between morphological phenotypic traits measured in this study. Values below the central diagonal axis relate to correlations under control conditions, and values above relate to waterlogging conditions (DOC 34 kb)

122_2010_1473_MOESM3_ESM.ppt

S3 Frequency distributions of all morphological phenotypic traits measured in this experiment. Both control and waterlogging distributions are located on the same graph for that trait. Mean AU6 parental values are indicated by arrows (PPT 287 kb)

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Pearson, A., Cogan, N.O.I., Baillie, R.C. et al. Identification of QTLs for morphological traits influencing waterlogging tolerance in perennial ryegrass (Lolium perenne L.). Theor Appl Genet 122, 609–622 (2011). https://doi.org/10.1007/s00122-010-1473-8

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