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Functionally associated molecular genetic marker map construction in perennial ryegrass (Lolium perenne L.)

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An Erratum to this article was published on 18 February 2005

Abstract

A molecular marker-based map of perennial ryegrass (Lolium perenne L.) has been constructed through the use of polymorphisms associated with expressed sequence tags (ESTs). A pair-cross between genotypes from a North African ecotype and the cultivar Aurora was used to generate a two-way pseudo-testcross population. A selection of 157 cDNAs assigned to eight different functional categories associated with agronomically important biological processes was used to detect polymorphic EST•RFLP loci in the F1(NA6 í AU6) population. A comprehensive set of EST•SSR markers was developed from the analysis of 14,767 unigenes, with 310 primer pairs showing efficient amplification and detecting 113 polymorphic loci. Two parental genetic maps were produced: the NA6 genetic map contains 88 EST•RFLP and 71 EST•SSR loci with a total map length of 963 cM, while the AU6 genetic map contains 67 EST•RFLP and 58 EST•SSR loci with a total map length of 757 cM. Bridging loci permitted the alignment of homologous chromosomes between the parental maps, and a sub-set of genomic DNA-derived SSRs was used to relate linkage groups to the perennial ryegrass reference map. Regions of segregation distortion were identified, in some instances in common with other perennial ryegrass maps. The EST-derived marker-based map provides the basis for in silico comparative genetic mapping, as well as the evaluation of co-location between QTLs and functionally associated genetic loci.

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Acknowledgements

We gratefully recognise technical support provided by Benjamin Franzmayr and Deborah Knox. The authors thank Prof. Michael Hayward for his careful critical reading of the manuscript. This work was performed within the Pasture Plant Genomics Program co-funded by the Department of Primary Industries, Victoria, Australia and AgResearch Limited, New Zealand. Funding was provided to AgResearch Ltd by the New Zealand Foundation for Research, Science and Technology under contract number C10X0203. Genetic mapping family development and genomic DNA-derived SSR genotyping was supported by the Molecular Plant Breeding Cooperative Research Centre (CRC). All experiments conducted during this study comply with current Australian laws.

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Author notes

  1. L. J. Hughes

    Present address: School of Biological Sciences and Biotechnology, Division of Science and Engineering, Murdoch University, South Street, Murdoch, WA, 6150, Australia

  2. E. S. Jones

    Present address: Crop Genetics Research and Development, Pioneer Hi-Bred International, 7300 NW 62nd Avenue, Johnston, IA, 50131-1004, USA

  3. K. M. Guthridge

    Present address: Cell Cycle and Development Laboratory, Peter MacCallum Cancer Centre, St. Andrew
tm)s Place, East Melbourne, VIC, 3002, Australia

  4. T. Sawbridge

    Present address: Molecular Bioscience Technologies, Department of Primary Industries and Fisheries, Queensland Biosciences Precinct, The University of Queensland, Level 6 North Tower, 306 Carmody Road, St. Lucia, 4072, Australia

Authors and Affiliations

  1. Grasslands Research Centre, AgResearch Ltd., Private Bag 11008, Palmerston North, New Zealand

    M. J. Faville & G. T. Bryan

  2. Primary Industries Research Victoria, Plant Biotechnology Centre, La Trobe University, Bundoora, VIC, 3086, Australia

    A. C. Vecchies, M. C. Drayton, L. J. Hughes, E. S. Jones, K. M. Guthridge, T. Sawbridge, G. C. Spangenberg & J. W. Forster

  3. Molecular Plant Breeding Cooperative Research Centre, Australia

    A. C. Vecchies, M. C. Drayton, L. J. Hughes, E. S. Jones, K. M. Guthridge, K. F. Smith, T. Sawbridge, G. C. Spangenberg & J. W. Forster

  4. AgResearch Limited, School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand

    M. Schreiber

  5. Primary Industries Research Victoria, Hamilton Centre, Private Bag 105, Hamilton, VIC, 3300, Australia

    K. F. Smith

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Correspondence to J. W. Forster.

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Communicated by G. Wenzel

An erratum to this article can be found at http://dx.doi.org/10.1007/s00122-005-1959-y

M.J. Faville and A.C. Vecchies contributed equally to this work.

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Faville, M.J., Vecchies, A.C., Schreiber, M. et al. Functionally associated molecular genetic marker map construction in perennial ryegrass (Lolium perenne L.). Theor Appl Genet 110, 12–32 (2004). https://doi.org/10.1007/s00122-004-1785-7

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