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Mapping QTLs and candidate genes for rice root traits under different water-supply conditions and comparative analysis across three populations

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Abstract

To investigate the genetic factors underlying constitutive and adaptive morphological traits of roots under different water-supply conditions, a recombinant inbred line (RIL) population derived from a cross between the lowland rice variety IR1552 and the upland rice variety Azucena with 249 molecular markers, was used in cylindrical-pot experiments. Eighteen QTLs were detected for seminal root length (SRL), adventitious root number (ARN), and lateral root length (LRL) and lateral root number (LRN) on the seminal root at a soil depth of from 3 to 6 cm under flooding and upland conditions. One identical QTL was detected under both flooding and upland conditions. The relative parameters under the two water-supply conditions were also used for QTL analysis. Five QTLs for upland induced variations in the traits were detected with the positive alleles from Azucena. A comparative analysis was performed for the QTLs detected in this study and those reported from two other populations with Azucena as a parent. Several identical QTLs for root elongation were found across the three populations with positive alleles from Azucena. Candidate genes were screened from ESTs and cDNA-AFLP clones for comparative mapping with the detected QTLs. Two genes for cell expansion, OsEXP2 and endo-1,4-β-D-glucanase EGase, and four cDNA-AFLP clones from root tissues of Azucena, were mapped on the intervals carrying the QTLs for SRL and LRL under upland conditions, respectively.

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Acknowledgements

This research was supported by National Key Basic Research Special Foundation of P. R. China, No.G1999011700.

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  1. B. S. Zheng

    Present address: College of Life Science, Zhejiang Forestry University, 311300, Lin An, P. R. China

Authors and Affiliations

  1. The State Key laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, 310029, Hangzhou, P. R. China

    B. S. Zheng, L. Yang, W. P. Zhang, C. Z. Mao, Y. R. Wu, K. K. Yi, F. Y. Liu & P. Wu

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  1. B. S. Zheng
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  2. L. Yang
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Correspondence to P. Wu.

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Communicated by H.C. Becker

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Zheng, B.S., Yang, L., Zhang, W.P. et al. Mapping QTLs and candidate genes for rice root traits under different water-supply conditions and comparative analysis across three populations. Theor Appl Genet 107, 1505–1515 (2003). https://doi.org/10.1007/s00122-003-1390-1

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  • DOI: https://doi.org/10.1007/s00122-003-1390-1

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