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Mapping of quantitative trait loci and development of allele-specific markers for seed weight in Brassica napus

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Abstract

Seed weight is an important component of grain yield in oilseed rape (Brassica napus L.), but the genetic basis for the important quantitative trait is still not clear. In order to identify the genes for seed weight in oilseed rape, QTL mapping for thousand seed weight (TSW) was conducted with a doubled haploid (DH) population and an F2 population. A complete linkage map of the DH population was constructed using 297 simple sequence repeat (SSR) markers. Among nine TSW QTLs detected, two major QTLs, TSWA7a and TSWA7b, were stably identified across years and collectively explained 27.6–37.9% of the trait variation in the DH population. No significant epistatic interactions for TSW detected in the DH population indicate that the seed weight variation may be primarily attributed to additive effects. The stability and significance of TSWA7a and TSWA7b were further validated in the F2 population with different genetic backgrounds. By cloning BnMINI3a and BnTTG2a, two B. napus homologous genes to Arabidopsis thaliana, allele-specific markers were developed for TSWA5b and TSWA5c, two TSW QTLs on A5, respectively. The importance of the major and minor QTLs identified was further demonstrated by analysis of the allelic effects on TSW in the DH population.

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Acknowledgments

The authors thank Drs. Jinling Meng, Jinxin Tu and Jianyi Zhao for providing SSR markers. The authors also appreciate three anonymous reviewers for their valuable comments and suggestions on the manuscript. This research was financially supported by National Basic Research Program (2006CB101604), National High-tech R&D Program (2006AA101A113) from Ministry of Science and Technology of China, National Natural Science Foundation of China (No. 30623012) and Science Foundation for the Youth Scholars of Ministry of Education of China (No. 20070504031).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Chuchuan Fan, Guangqin Cai, Jie Qin, Qingyuan Li, Minggui Yang, Jianzhong Wu, Tingdong Fu, Kede Liu & Yongming Zhou

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  1. Chuchuan Fan
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  2. Guangqin Cai
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  3. Jie Qin
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  4. Qingyuan Li
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  8. Kede Liu
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Correspondence to Kede Liu or Yongming Zhou.

Additional information

Communicated by H. Becker.

C. Fan, G. Cai and J. Qin contributed equally to this work.

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Fan, C., Cai, G., Qin, J. et al. Mapping of quantitative trait loci and development of allele-specific markers for seed weight in Brassica napus . Theor Appl Genet 121, 1289–1301 (2010). https://doi.org/10.1007/s00122-010-1388-4

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