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Quantitative trait analysis of seed yield and other complex traits in hybrid spring rapeseed (Brassica napus L.): 2. Identification of alleles from unadapted germplasm

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Abstract

Unadapted germplasm may contain alleles that could improve hybrid cultivars of spring oilseed Brassica napus. Quantitative trait loci (QTL) mapping was used to identify potentially useful alleles from two unadapted germplasm sources, a Chinese winter cultivar and a re-synthesized B. napus, that increase seed yield when introgressed into a B. napus spring hybrid combination. Two populations of 160 doubled haploid (DH) lines were created from crosses between the unadapted germplasm source and a genetically engineered male-fertility restorer line (P1804). A genetically engineered male-sterile tester line was used to create hybrids with each DH line (testcrosses). The two DH line populations were evaluated in two environments and the two testcross populations were evaluated in three or four environments for seed yield and other agronomic traits. Several genomic regions were found in the two testcross populations which contained QTL for seed yield. The map positions of QTL for days to flowering and resistance to a bacterial leaf blight disease coincided with QTL for seed yield and other agronomic traits, suggesting the occurrence of pleiotropic or linked effects. For two hybrid seed yield QTL, the favorable alleles increasing seed yield originated from the unadapted parents, and one of these QTL was detected in multiple environments and in both populations. In this QTL region, a chromosome rearrangement was identified in P1804, which may have affected seed yield.

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Acknowledgements

We thank researchers at Bayer CropScience for their assistance, particularly Tom Schuler, Stewart Brandt, and Hieronim Polewicz for seed production and field trials, and Debbie Doell for microspore culture and plantlet development. We also thank Robert Vogelzang for his technical assistance. JAU was funded in part by UW Pioneer Plant Breeding Fellowship 1999–2000 and PAQ was funded by a scholarship from Consejo de Desarrollo Científico y Humanístico, Universidad Central de Venezuela, Government of Venezuela. Additional funding was provided by North Central Biotechnical Initiative and USDA-NRI grant # 98–353006–6286 to T.C.O.

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  1. Joshua A. Udall

    Present address: Department of Evolution, Ecology and Organismal Biology, Iowa State University, Ames, IA, 50011, USA

  2. Thomas C. Osborn

    Present address: Seminis Vegetable Seeds, 37437 State Highway 16, Woodland, CA, 95695, USA

Authors and Affiliations

  1. Plant Breeding and Plant Genetics Program, and Department of Agronomy, University of Wisconsin, Madison, WI, 53706, USA

    Joshua A. Udall, Pablo A. Quijada & Thomas C. Osborn

  2. Bayer BioScience, Nazarethse Steenweg 77, 9800, Astene, Belgium

    Bart Lambert

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  1. Joshua A. Udall
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  2. Pablo A. Quijada
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Correspondence to Thomas C. Osborn.

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Communicated by S. J. Knapp

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Udall, J.A., Quijada, P.A., Lambert, B. et al. Quantitative trait analysis of seed yield and other complex traits in hybrid spring rapeseed (Brassica napus L.): 2. Identification of alleles from unadapted germplasm. Theor Appl Genet 113, 597–609 (2006). https://doi.org/10.1007/s00122-006-0324-0

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