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One hundred and one new microsatellite loci derived from ESTs (EST-SSRs) in bread wheat

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Abstract

Four hundred and seventy-eight microsatellite markers derived from expressed sequence tags (EST-SSRs) were screened among three mapping populations (W-7984×Opata 85, WOpop; Lumai×Hanxuan, LHpop; Wenmai×Shanhongmai, WSpop). The number of polymorphic EST-SSR primer pairs found in WOpop, LHpop and WSpop was 92, 58 and 29 respectively. A total of 101 EST-SSR loci amplified from 88 primer sets were distributed over the 20 chromosomes of the reference maps (no markers were located on chromosome 4B). These 101 mapped EST-SSR markers add to the existing 450 microsatellite loci previously mapped in bread wheat. Seventy-four of the 101 loci showed significant similarities to known genes, including 24 genes involved in metabolism, 4 in cellular structures, 9 in stress resistance, 12 in transcription, 2 in development, 2 transporters and 21 storage proteins. Besides gliadin and glutenin, most of the 53 genes with putative functions were mapped for the first time by EST-SSR markers in bread wheat. Sequence alignment of the mapped wheat EST-SSR loci allowed tentative assignment of functionality to the other members of grasses family. Colinearity combined with homology information offers an attractive approach to comparative genomics.

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Acknowledgements

The authors gratefully acknowledge two anonymous referees for their constructive comments and Dr. Leung He (IRRI) for critically reading the manuscript.

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Authors and Affiliations

  1. Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture, Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, 100081, Beijing, China

    L. F. Gao, R. L. Jing, N. X. Huo, Y. Li, X. P. Li, R. H. Zhou, X. P. Chang, J. F. Tang & J. Z. Jia

  2. Department of Agronomy, Heibei Agricultural University, 171001, Bao ding, China

    Y. Li & Z. Y. Ma

  3. Northwest Sci-Tech University of Agriculture and Forestry, 712100, Yang ling, China

    X. P. Li

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  1. L. F. Gao
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  2. R. L. Jing
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  3. N. X. Huo
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  4. Y. Li
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  5. X. P. Li
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  6. R. H. Zhou
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  8. J. F. Tang
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  9. Z. Y. Ma
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  10. J. Z. Jia
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Correspondence to J. Z. Jia.

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Communicated by R. Hagemann

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Gao, L.F., Jing, R.L., Huo, N.X. et al. One hundred and one new microsatellite loci derived from ESTs (EST-SSRs) in bread wheat. Theor Appl Genet 108, 1392–1400 (2004). https://doi.org/10.1007/s00122-003-1554-z

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  • DOI: https://doi.org/10.1007/s00122-003-1554-z

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