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Genetic diversity of Aegilops tauschii accessions and its relationship with tetraploid and hexaploid wheat using retrotransposon-based molecular markers

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Abstract

Aegilops is known as the ancestor species of wheat and has a major role in the evolution of wheat. Considering the importance of this species and the fact that Iran is the center of the distribution, fifteen IRAP and REMAP markers were used to evaluate the genetic diversity of Ae. tauschii accessions from different regions of Iran and its relationship with Triticum durum and Triticum aestivum. A high level of polymorphism (99%) was observed for both retrotransposon markers. Polymorphism information content, the effective number of alleles (Ne), Nei’s genetic diversity (H), and Shannon’s information index (I) for IRAP markers were more than REMAP markers. The most genetic diversity was detected in Ae. tauschii accessions. The cluster analysis separated the accessions into three distinct groups. Ae. tauschii accessions from Golestan province grouped in the same cluster with T. aestivum genotypes, indicating that these accessions are probably more like to the progenitor of the bread wheat D genome. Our results revealed that retrotransposon-based molecular markers could be used as the appropriate and reliable marker system to evaluate genetic diversity and relationship in Triticum and Aegilops accessions.

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Acknowledgements

The authors are grateful to the Department of Genetics Research and National Plant Gene Bank of Iran, Seed and plant improvement institute, Karaj, Iran for providing the genotypes seeds.

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Authors and Affiliations

  1. Department of Plant Production and Genetic Engineering, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

    Samira Safiyar & Babak Rabiei

  2. Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

    Ali Aalami

  3. Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, Urmia University, Urmia, Iran

    Babak Abdollahi Mandoulakani

  4. Department of Plant Breeding, Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Karaj, Iran

    Mojtaba Kordrostami

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  1. Samira Safiyar
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  2. Ali Aalami
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  3. Babak Abdollahi Mandoulakani
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  4. Babak Rabiei
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  5. Mojtaba Kordrostami
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Correspondence to Ali Aalami.

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Communicated by M. Molnár-Láng.

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Safiyar, S., Aalami, A., Abdollahi Mandoulakani, B. et al. Genetic diversity of Aegilops tauschii accessions and its relationship with tetraploid and hexaploid wheat using retrotransposon-based molecular markers. CEREAL RESEARCH COMMUNICATIONS 50, 219–226 (2022). https://doi.org/10.1007/s42976-021-00167-9

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  • DOI: https://doi.org/10.1007/s42976-021-00167-9

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