Abstract
Doubled haploid (DH) technology in cereals has emerged as a promising tool for accelerating the development of completely homozygous lines in a much shorter time than conventional breeding methods. The rapid doubled haploid line production method reduces the breeding cycle’s length and increases the genetic gain. In cereals, mainly conventional approaches, such as in vitro and in planta methods, have been employed to generate haploids that are subsequently converted to doubled haploids by spontaneous or induced chromosome doubling. The use of in vitro methods is limited owing to genotypic specificity and tissue culture dependence. In planta methods prevent the need for difficult tissue culture procedures and enhance the haploid recovery rate. Further, haploid induction through inducer lines can generate the haploid embryos when crossed with other plants. In cereals, the availability of commercially usable maternal and paternal haploid inducers at present is limited to maize. Mutations in the genomic region coding for phospholipases, MTL, NLD and PLA1, have been established to be responsible for the haploid induction in maize and other cereals such as rice. With the technology advancement, improved and highly efficient genetic engineering methods lead to the development of haploid inducers. MTL gene is targeted with CRISPR/Cas9 for haploid induction in maize and rice. The present updates on the genetic and molecular basis of doubled haploidy have opened new cereal breeding prospects for undertaking targeted and precise genetic improvement programmes in a shorter time. Doubled haploidy can be integrated with marker-assisted breeding to fix favourable alleles of multiple traits in a single DH line. The technology can also be used for unlocking the genetic variability present in the unexploited germplasm/landraces, CMS line production and reverse breeding. DH breeding’s future is promising due to the availability of robust DH production protocols and closer integration with marker-assisted technologies.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Agnieszka K, Adela A (2010) In vitro culture of unfertilized ovules in carrot (Daucus carota L.). Plant Cell Tissue Organ Cult 102:309–319
Alam SN, Cohen MB (1998) Detection and analysis of QTLs for resistance to the brown planthopper, Nilaparvata lugens, in a doubled-haploid rice population. Theor Appl Genet 97:1370–1379. https://doi.org/10.1007/s001220051031
Al-Ashkar A, El-Hendawy A-S, El-Kafafi S, Seleiman MF (2019) Detecting salt tolerance in doubled haploid wheat lines. Agronomy 9(4):211. https://doi.org/10.3390/agronomy9040211
Anderson SB, Due IK, Olsen A (1987) The response of anther culture in a genetically wide material of winter wheat (Triticum aestivum L.). Plant Breed 99:181–186. https://doi.org/10.1111/j.1439-0523.1987.tb01170.x
Arumuganathan K, Earle ED (1991a) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218. https://doi.org/10.1007/BF02672069
Arumuganathan K, Earle ED (1991b) Estimation of nuclear DNA contents of plants by flow cytometry. Plant Mol Biol Rep 9:229–241. https://doi.org/10.1038/nprot.2007.310
Asif MA, Schilling RK, Tilbrook J, Brien C, Dowling K, Rabie H et al (2018) Mapping of novel salt tolerance QTL in an Excalibur × Kukri doubled haploid wheat population. Theor Appl Genet 131:2179–2196. https://doi.org/10.1007/s00122-018-3146-y
Bae CH, Lee YI, Lim YP, Seo YW, Lee DJ, Yang DC, Lee HY (2002) Detection of herbicide tolerant cell lines from c-ray irradiated cell cultures in rice (Oryza sativa L. cv. Ilpumbyeo). J Plant Biotech 4:123–127
Bains NS, Mangat GS, Singh K, Nanda GS (1998) A simple technique for the identification of embryo carrying seeds from wheat × maize crosses prior to dissection. Plant Breed 117:191–192
Barclay IR (1975) High frequencies of haploid production in wheat (Triticum aestivum) by chromosome elimination. Nature 256:410–411. https://doi.org/10.1038/256410a0
Barret P, Brinkmann M, Beckert M (2008) A major locus expressed in the male gametophyte with incomplete penetrance is responsible for in situ gynogenesis in maize. Theor Appl Genet 117:581–594. https://doi.org/10.1007/s00122-008-0803-6
Barro F, Fernandez‐Escobar J, De la Vega M, Martin A (2001) Doubled haploid lines of Brassica carinata with modified erucic acid content through mutagenesis by EMS treatment of isolated microspores. Plant Breed 120:262–264. https://doi.org/10.1046/j.1439-0523.2001.00602.x
Barro F, Fernandez-Escobar J, De La Vega M, Martin A (2002) Modification of glucosinolate and erucic acid contents in doubled haploid lines of Brassica carinata by UV treatment of isolated microspores. Euphytica 129:1–6. https://doi.org/10.1023/A:1021578318098
Basu SK, Eudes F, Kovalchuk I (2010) Role of recA/RAD51 gene family in homologous recombination repair and genetic engineering of transgenic plants. In: Kumar A, Sopory S (eds) Applications of plant biotechnology: In vitro propagation, plant transformation and secondary metabolite production. I.K. International Publishing House Pvt Ltd., New Delhi, pp 231–255
Battistelli GM, VonPinho RG, Justus A, Couto EG, Balestre M (2013) Production and identification of doubled haploids in tropical maize. Gen Mol Res 12:4230–4242. https://doi.org/10.4238/2013.October.7.9
Belicuas PR, Guimarães CT, Paiva LV, Duarte JM, Maluf WR, Paiva E (2007) Androgenetic haploids and SSR markers as tools for the development of tropical maize hybrids. Euphytica 156:95–102. https://doi.org/10.1007/s10681-007-9356-z
Bernardo R (2009) Should maize doubled haploids be induced among F1 or F2 plants? Theor Appl Genet 119:255–262. https://doi.org/10.1007/s00122-009-1034-1
Bhowmik P, Ellison E, Polley B, Bollina V, Kulkarni M, Ghanbarnia K, Kagale S (2018) Targeted mutagenesis in wheat microspores using CRISPR/Cas9. Sci Rep 8:6502. https://doi.org/10.1038/s41598-018-24690-8
Blakeslee AF, Belling J, Farnham ME, Bergner AD (1922) A haploid mutant in the Jimson weed, Datura stramonium. Science 55:646–647
Boerman NA, Frei UK, Lübberstedt T (2020) Impact of spontaneous haploid genome doubling in maize breeding. Plan Theory 9(3):369. https://doi.org/10.3390/plants9030369
Boote BW, Freppon DJ, De La Fuente GN et al (2016) Haploid differentiation in maize kernels based on fluorescence imaging. Plant Breed 135:439–445
Britt AB, Kuppu S (2016) Cenh3: an emerging player in haploid induction technology. Front Plant Sci 7:357. https://doi.org/10.3389/fpls.2016.00357
Buyser J, Henry Y, Lonnet P, Hertzog R, Hespel A (1987) ‘Florin’: a doubled haploid wheat variety developed by the anther culture method. Plant Breed 98:53–56. https://doi.org/10.1111/J.1439-0523.1987.TB01089.X
Bylich VG, Chalyk ST (1996) Existence of pollen grains with a pair of morphologically different sperm nuclei as a possible cause of the haploid-inducing capacity in ZMS line. Maize Genet Coop News Lett 70:33–33
Calayugan MIC, Formantes AK, Amparado A, Descalsota-Empleo GI, Nha CT, Inabangan-Asilo MA et al (2020) Genetic analysis of agronomic traits and grain iron and zinc concentrations in a doubled haploid population of rice (Oryza sativa L.). Sci Rep 10:1–14. https://doi.org/10.1038/s41598-020-59184-z
Castillo AM, Cistue L, Valles MP, Sanz L, Romagosa I, Molina-Cano JL (2001) Efficient production of androgenic doubled-haploid mutants in barley by the application of sodium azide to anther and microspore cultures. Plant Cell Rep 20:105–111
Castillo AM, Cistué L, Vallés MP et al (2009) Chromosome doubling in monocots. In: Touraev A, Forster BP, Jain SM (eds) Advances in haploid production in higher plants. Springer, Berlin, pp 329–338
Chaikam V, Prasanna BM (2012) Maternal haploid detection using anthocyanin markers. In: Prasanna BM, Chaikam V, Mahuku G (eds) Doubled haploid technology in maize breeding: theory and practice. CIMMYT, Mexico, pp 20–23
Chaikam V, Nair SK, Babu R et al (2015) Analysis of effectiveness of R1-nj anthocyanin marker for in vivo haploid identification in maize and molecular markers for predicting the inhibition of R1-nj expression. Theor Appl Genet 128:159–171. https://doi.org/10.1007/s00122-014-2419-3
Chaikam V, Martinez L, Melchinger AE et al (2016) Development and validation of red root marker-based haploid inducers in maize. Crop Sci 56:1678–1688. https://doi.org/10.2135/cropsci2015.10.0653
Chaikam V, Lopez LA, Martinez L et al (2017) Identification of in vivo induced maternal haploids in maize using seedling traits. Euphytica 213:177. https://doi.org/10.1007/s10681-017-1968-3
Chaikam V, Gowda M, Nair SK, Melchinger AE, Boddupalli PM (2019a) Genome-wide association study to identify genomic regions influencing spontaneous fertility in maize haploids. Euphytica 215:138. https://doi.org/10.1007/s10681-019-2459-5
Chaikam V, Molenaar W, Melchinger AE et al (2019b) Doubled haploid technology for line development in maize: technical advances and prospects. Theor Appl Genet 132:3227–3243. https://doi.org/10.1007/s00122-019-03433-x
Chalyk ST (1994) Properties of maternal haploid maize plants and potential application to maize breeding. Euphytica 79:13–18. https://doi.org/10.1007/BF00023571
Chase SS (1969) Monoploids and monoploid-derivatives of maize (Zea mays L.). Bot Rev 35:117–168. https://doi.org/10.1007/BF02858912
Chaudhary HK, Sethi GS, Singh S, Pratap A, Sharma S (2005) Efficient haploid induction in wheat by using pollen of Imperata cylindrica. Plant Breed 124:96–98. https://doi.org/10.1111/j.1439-0523.2004.01034.x
Chaudhary HK, Tayeng T, Kaila V, Rather SA (2013) Enhancing the efficiency of wide hybridization mediated chromosome engineering for high precision crop improvement with special reference to wheat × Imperata cylindrica system. Nucleus 56:7–14. https://doi.org/10.1007/s13237-013-0077-5
Chaudhary HK, Badiyala A, Jamwal NS (2015) New frontiers in doubled haploidy breeding in wheat. Agric Res J 52(4):1–12. https://doi.org/10.5958/2395-146X.2015.00053.8
Chaudhary HK, Sharma P, Manoj NV, Singh K (2019) New frontiers in chromosome elimination- mediated doubled haploidy breeding: Focus on speed breeding in bread and durum wheat. Indian J Genet 79:254–263. https://doi.org/10.5958/2395-146X.2015.00053.8
Chauhan H, Khurana P (2011) Use of doubled haploid technology for development of stable drought tolerant bread wheat (Triticum aestivum L.) transgenics. Plant Biotechnol J 9:408–417. https://doi.org/10.1111/j.1467-7652.2010.00561.x
Choe E, Carbonero CH, Mulvaney K, Rayburn AL, Mumm RH (2012) Improving in vivo maize doubled haploid production efficiency through early detection of false positives. Plant Breed 131:399–401. https://doi.org/10.1111/j.1439-0523.2012.01962.x
Chu CG, Friesen TL, Xu SS, Faris JD, Kolmer JA (2009) Identification of novel QTLs for seedling and adult plant leaf rust resistance in a wheat doubled haploid population. Theor Appl Genet 119:263–269. https://doi.org/10.1007/s00122-009-1035-0
Clapham D (1973) Haploid Hordeum plants from anthers in vitro. Z Pflanzenzüchtg 69:142–155
Coe EH (1959) A line of maize with high haploid frequency. Am Nat 93:381–382
Coe EH (1994) Anthocyanin genetics. In: Freeling M, Walbot V (eds) The maize handbook. Springer- Verlag, New York, pp 279–281
Couto EGO, Davide LMC, Bustamante FO, Von Pinho RG, Silva TN (2013) Identification of haploid maize by flow cytometry, morphological and molecular markers. Ciên Agrotecnol 37:25–31. https://doi.org/10.1590/S1413-70542013000.100003
Dashti H, Yazdisamadi B, Reza Bihamta M, Reza Naghavi M, Yazdi-samadi B, Ghannadha M et al (2007) QTL analysis for drought resistance in wheat using doubled haploid lines. Int J Agric Biol 9:98–102. http://www.fspublishers.org/published_papers/71708_..pdf. Accessed 22 April 2021
Davies DR (1974) Chromosome elimination in inter-specific hybrids. Heredity 32:267–270
Davrieux F, Allal F, Piombo G, Kelly B, Okulo JB, Thiam M, Bouvet JM (2010) Near infrared spectroscopy for high- throughput characterization of shea tree (Vitellaria paradoxa) nut fat profiles. J Agric Food Chem 58:7811–7819. https://doi.org/10.1021/jf100409v
De La Fuente GN, Frei UK, Trampe B, Nettleton D, Zhang W, Lubberstedt T (2018) A diallel analysis of a maize donor population response to in vivo maternal haploid induction I: inducibility. Crop Sci 58:1830–1837. https://doi.org/10.2135/cropsci2017.05.0285
Deimling S, Röber F, Geiger HH (1997) Methodik und genetik der in vivo-haploideninduktion bei mais. Vor Pflanzenzüchtg 38:203–224
DePauw RM, Townley-Smith TF, Humphreys G (2005) Lillian hard red spring wheat. Can J Plant Sci 85:397–401. https://doi.org/10.4141/P04-137
Derakhshani B, Jafary H, Zanjani BM, Hasanpur K, Mishina K, Tanaka T et al (2020) Combined QTL mapping and RNA-Seq profiling reveals candidate genes associated with cadmium tolerance in barley. PLoS One 15:e0230820. https://doi.org/10.1371/journal.pone.0230820
Devaux P, Pickering R (2005) Haploids in the improvement of Poaceae. In: Haploids in crop improvement II. Springer, Berlin, pp 215–242
Dirks R, Dun K, Snoo CB, de Berg M, van den Lelivelt CLC, Voermans W et al (2009) Reverse breeding: a novel breeding approach based on engineered meiosis. Plant Biotechnol J 7:837. https://doi.org/10.1111/J.1467-7652.2009.00450.X
Dong X, Xu X, Miao J et al (2013) Fine mapping of qhir1 influencing in vivo haploid induction in maize. Theor Appl Genet 126:1713–1720. https://doi.org/10.1007/s00122-013-2086-9
Dunwell JM (2010) Haploids in flowering plants: origins and exploitation. Plant Biotechnol J 8:377–424. https://doi.org/10.1111/j.1467-7652.2009.00498.x
Dwivedi SL, Britt AB, Tripathi L, Sharma S, Upadhyaya HD, Ortiz R (2015) Haploids: constraints and opportunities in plant breeding. Biotechnol Adv 33:812–829
Eder J, Chalyk ST (2002) In vivo haploid induction in maize. Theor Appl Genet 104:703–708. https://doi.org/10.1007/s00122-001-0773-4
Ekiz H, Konzak CF (1991) Nuclear and cytoplasmic control of anther culture response in wheat. III Common wheat crosses Crop Sci 31:1432–1436
El-Hennawy MA, Abdalla AF, Shafey SA, Al-Ashkar IM (2011) Production of doubled haploid wheat lines (Triticum aestivum L.) using anther culture technique. Ann Agri Sci 56:63–72. https://doi.org/10.1016/j.aoas.2011.05.008
Evans MMS (2007) The indeterminate gametophyte1 gene of maize encodes a LOB domain protein required for embryo sac and leaf development. Plant Cell 19:46–62. https://doi.org/10.1105/tpc.106.047506
Ferrie AMR, Caswell KL (2011) Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell Tissue Organ Cult 104:301–309. https://doi.org/10.1007/s11240-010-9800-y
Finch RA (1983) Tissue-specific elimination of alternative whole parental genomes in one barley hybrid. Chromosoma 88:386–393. https://doi.org/10.1007/BF00285861
Finch RA, Bennett MD (1982) The mechanism of somatic chromosome elimination in Hordeum. In: Brandham PE, Bennett MD (eds) Kew Chromosome Conference II. Allen & Unwin, London, pp 146–153
Fischer E (2004) Molecular genetic studies on the occurrence of paternal DNA transmission during in vivo haploid induction in maize (Zea mays). Doctoral dissertation. University of Hohenheim, Germany
Forster BP, Heberle-Bors E, Kasha KJ, Touraev A (2007) The resurgence of haploids in higher plants. Trends Plant Sci 12:368–375. https://doi.org/10.1016/j.tplants.2007.06.007
Fox G, Wu A, Yiran L, Force L (2013) Variation in caffeine concentration in single coffee beans. J Agric Food Chem 61(45):10772–10778. https://doi.org/10.1021/jf4011388
Gahlaut V, Jaiswal V, Tyagi BS, Singh G, Sareen S, Balyan HS et al (2017) QTL mapping for nine drought-responsive agronomic traits in bread wheat under irrigated and rain-fed environments. PLoS One 12:e0182857. https://doi.org/10.1371/journal.pone.0182857
Gaillard A, Vergne P, Beckert M (1991) Optimization of maize microspore isolation and culture conditions for reliable plant regeneration. Plant Cell Rep 10:55–58. https://doi.org/10.1007/BF00236456
Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozbady E (1983) Rapid flow cytometric analysis of cell cycle in intact plant tissue. Science 220:1049–1051. https://doi.org/10.1126/science.220.4601.1049
García-llamas C, Martín A, Ballesteros J (2004) Differences among auxin treatments on haploid production in durum wheat × maize crosses. Plant Cell Rep 23:46–49. https://doi.org/10.1007/s00299-004-0786-y
Geiger HH (2009) Doubled haploids. In: Bennetzen JL, Hake S (eds) Handbook of maize. Springer, New York, pp 641–657. https://doi.org/10.1007/978-0-387-77863-1_32
Geiger HH, Roux SR, Deimling S (1994) Herbicide resistance as a marker in screening for maternal haploids. Maize Genet Coop News Lett 68:99
Genovesi A, Clint W (1979) Improved rate of callus and green plant production from rice anther culture following cold shock. Crop Sci 19:662–664
Germanà MA (2011) Gametic embryogenesis and haploid technology as valuable support to plant breeding. Plant Cell Rep 30:839–857. https://doi.org/10.1007/s00299-011-1061-7
Gernand D, Rutten T, Varshney A, Rubtsova M, Prodanovic S et al (2005) Uniparental chromosome elimination at mitosis and interphase in wheat and pearl millet crosses involves micronucleus formation, progressive heterochromatinization, and DNA fragmentation. Plant Cell 17:2431–2438. https://doi.org/10.1105/tpc.105.034249
Gernand D, Rutten T, Pickering R, Houben A (2006) Elimination of chromosomes in Hordeum vulgare × H. bulbosum crosses at mitosis and interphase involves micronucleus formation and progressive heterochromatinization. Cytogenet Genome Res 114:169–174. https://doi.org/10.1159/000093334
Gilles LM, Khaled A, Laffaire J et al (2017) Loss of pollen-specific phospholipase NOT LIKE DAD triggers gynogenesis in maize. EMBO J 36:707–717. https://doi.org/10.15252/embj.201796603
Gous PW, Christopher LH, Franckowiak J, Fox GP (2016) Discovery of QTL for stay-green and heat-stress in barley (Hordeum vulgare) grown under simulated abiotic stress conditions. Euphytica 207:305–317. https://doi.org/10.1007/s10681-015-1542-9
Graf RJ, Beres BL, Laroche A (2013) Emerson hard red winter wheat. Can J Plant Sci 93:741–748. https://doi.org/10.4141/cjps2012-262
Grauda D, Lepse N, Strazdina V et al (2010) Obtaining of doubled haploid lines by anther culture method for the Latvian wheat breeding. Agron Res 8:545–552
Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature 204:497–498. https://doi.org/10.1038/204497a0
Guha S, Maheshwari SC (1966) Cell division and differentiation of embryos in the pollen grains of Datura in vitro. Nature 212:97–98. https://doi.org/10.1038/212097a0
Gupta SB (1969) Duration of mitotic cycle and regulation of DNA replication in Nicotiana plumbaginifolia and a hybrid derivative of N. tabacum showing chromosome instability. Can J Genet Cytol 11:133–142. https://doi.org/10.1139/g69-017
Hai L, Guo H, Xiao S, Jiang G, Zhang X, Yan C et al (2005) Quantitative trait loci (QTL) of stem strength and related traits in a doubled-haploid population of wheat (Triticum aestivum L.). Euphytica 141:1–9. https://doi.org/10.1007/s10681-005-4713-2
Han H (1986) Wheat: improvement through anther culture Biotechnology in agriculture and forestry 2 by Bajaj YPS, vol 2. Springer, Berlin, pp 55–722. https://doi.org/10.1007/978-3-642-61625-9_3
Heszky LE, Simon-Kiss I (1992) The first plant variety of biotechnology origin in Hungary, registered in 1992. Hungar Agric Res 1:30–32
Hoekstra S, Van Zijderveld MH, Heidekamp E, Van der Mark E (1993) Microspore culture of Hordeum vulgare L.: the influence of density and osmolality. Plant Cell Rep 12:661–665. https://doi.org/10.1007/BF00233415
Hooghvorst I (2020) Opportunities and challenges in doubled haploids systems in cucurbits. Agron 10:1441. https://doi.org/10.3390/agronomy10091441
Hooghvorst I, Ribas P, Nogués S (2020) Chromosome doubling of androgenic haploid plantlets of rice (Oryza sativa) using antimitotic compounds. Plant Breed 139:754–761. https://doi.org/10.1111/pbr.12824
Hu TC, Ziauddin A, Simion E, Kasha KJ (1995) Isolated microspore culture of wheat (Triticum aestivum L.) in a defined media: I. Effects of pretreatment, isolation methods, and hormones. In Vitro Cell Dev Biol 31:79–83. https://doi.org/10.1007/BF02632241
Hua C, Chuan-xi MA, Yu-qiang Q (2008) Identification of morphology and cytology in wheat haploid through wheat × maize. J Nucl Agric Sci 22:127–130
Huang N, Parco A, Mew T, Magpantay G, McCouch S, Guiderdoni E et al (1997) RFLP mapping of isozymes, RAPD and QTLs for grain shape, brown planthopper resistance in a doubled haploid rice population. Mol Breed 3:105–113. https://doi.org/10.1023/A:1009683603862
Ilyas M, Ilyas N, Arshad M, Kazi AG, Kazi AM, Waheed A (2014) QTL mapping of wheat doubled haploids for chlorophyll content and chlorophyll fluorescence kinetics under drought stress imposed at anthesis stage. Pak J Bot 46:1889–1897
Inagaki MN, Mujeeb-Kazi A (1995) Comparison of polyhaploid production frequencies in crosses of hexaploid wheat with maize, pearl millet and sorghum. Breed Sci 45:157–161. https://doi.org/10.1270/jsbbs1951.45.157
Ishii T, Karimi-Ashtiyani R, Houben A (2016) Haploidization via chromosome elimination: means and mechanisms. Annu Rev Plant Biol 67:421–438. https://doi.org/10.1146/annurev-arplant-043014-114714
Jacquier NMA, Gilles LM, Pyott DE, Martinant JP, Rogowsky PM, Widiez T (2020) Puzzling out plant reproduction by haploid induction for innovations in plant breeding. Nat Plants 6:610–619. https://doi.org/10.1038/s41477-020-0664-9
Jiao Y, Li J, Li W, Chen M, Li M, Liu W et al (2020) QTL mapping and prediction of haploid male fertility traits in maize (Zea mays L.). Plan Theory 9:1–12. https://doi.org/10.3390/plants9070836
Jones RW, Reinot T, Frei UK, Tseng Y, Lübberstedt T, McClelland JF (2012) Selection of haploid maize kernels from hybrid kernels for plant breeding using near infrared spectroscopy and SIMCA analysis. Appl Spectrosc 66:447–450. https://doi.org/10.1366/2F11-06426
Joosen R, Cordewener J, Supena EDJ, Vorst O, Lammers M, Maliepaard C et al (2007) Combined transcriptome and proteome analysis identifies pathways and markers associated with the establishment of rapeseed microspore-derived embryo development. Plant Physiol 144:155–172. https://doi.org/10.1104/pp.107.098723
Kalinowska K, Chamas S, Unkel K, Demidov D, Lermontova I, Dresselhaus T, Kumlehn J, Dunemann F, Houben A (2019) State-of-the-art and novel developments of in vivo haploid technologies. Theor Appl Genet 132:593–605. https://doi.org/10.1007/s00122-018-3261-9
Kasha KJ, Kao KN (1970) High frequency haploid production in barley (Hordeum vulgare L.). Nature 225:874–876. https://doi.org/10.1038/225874a0
Kasha KJ, Maluszynski M (2003) Production of doubled haploids in crop plants. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plant. Kluwer Academic Publishers, Dordrecht, pp 1–4
Kebede AZ, Dhillon BS, Schipprack W et al (2011) Effect of source germplasm and season on the in vivo haploid induction rate in tropical maize. Euphytica 180:219–226. https://doi.org/10.1007/s10681-011-0376-3
Kelliher T, Starr D, Richbourg L et al (2017) MATRILINEAL, a sperm-specific phospholipase, triggers maize haploid induction. Nature 542:105–109. https://doi.org/10.1038/nature20827
Kelliher T, Starr D, Su X, Tang G, Chen Z, Carter J et al (2019) One-step genome editing of elite crop germplasm during haploid induction. Nat Biotechnol 37:287–292. https://doi.org/10.1038/s41587-019-0038-x
Kermicle JL (1969) Androgenesis conditioned by a mutation in maize. Science 166:1422–1424. https://doi.org/10.1126/science.166.3911.1422
Kermicle J (1973) Androgenesis and the indeterminate gametophyte mutation: source of the cytoplasm. Maize Genet Coop News Lett 47:208–209
Kermicle JL (1994) Indeterminate gametophyte (ig): biology and use. In: Freeling M, Walbot V (eds) The maize handbook. Springer, New York, pp 388–393. https://doi.org/10.1007/978-1-4612-2694-9_58
Khan AJ, Hassan S, Tariq M, Khan T (2001) Haploidy breeding and mutagenesis for drought tolerance in wheat. Euphytica 120:409–414. https://doi.org/10.1023/A:1017598202368
Khulbe RK, Pattanayak A, Panday V (2019) R1-nj expression in parental inbreds as a predictor of amenability of maize hybrids to R1-nj-based doubled haploid development. Indian J Genet 79:678–684. https://doi.org/10.31742/IJGPB.79.4.5
Khulbe RK, Pattanayak A, Lakshmi K, Bisht GS, Pant MC, Pandey V, Rohit K, Mishra NC (2020) Doubled haploid production in maize under submontane Himalayan conditions using R1-nj-based haploid inducer TAILP1. Indian J Genet 80:261–266. https://doi.org/10.31742/IJGPB.80.3.4
Kim DS, Lee IS, Jang CS, Hyun DY, Lee SJ, Seo YW, Lee YI (2003) Selection of 5-methyltryptophan and S-(2-aminoethyl)-L-cysteine resistant microspore-derived rice cell lines irradiated with gamma rays. J Plant Biotech 5:33–41
Kisana NS, Nkongolo KK, Quick JS, Johnson DL (1993) Production of doubled haploids by anther culture and wheat × maize method in a wheat breeding programme. Plant Breed 110:96–102
Kishore N, Chaudhary HK, Chahota RK, Kumar V, Sood SP, Jeberson S, Tayeng T (2011) Relative efficiency of the maize and Imperata cylindrica-mediated chromosome elimination approaches for induction of haploids of wheat-rye derivatives. Plant Breed 130:192–194. https://doi.org/10.1111/j.1439-0523.2010.01793.x
Kiviharju E, Moisander S, Laurila J (2005) Improved green plant regeneration rates from oat anther culture and the agronomic performance of some DH lines. Plant Cell Tissue Organ Cult 81:1–9. https://doi.org/10.1007/s11240-004-1560-0
Kleiber D, Prigge V, Melchinger AE, Burkard F, San Vicente F, Palomino G, Andrés Gordillo G (2012) Haploid fertility in temperate and tropical maize germplasm. Crop Sci 52:623–630. https://doi.org/10.2135/cropsci2011.07.0395
Kochevenko A, Jiang Y, Seiler C, Surdonja K, Kollers S, Reif JC et al (2018) Identification of QTL hot spots for malting quality in two elite breeding lines with distinct tolerance to abiotic stress. BMC Plant Biol 18:1–17. https://doi.org/10.1186/s12870-018-1323-4
Köhler F, Wenzel G (1985) Regeneration of isolated barley microspores in conditioned media and trials to characterise the responsible factor. J Plant Physiol 121:181–191. https://doi.org/10.1016/S0176-1617(85)80044-4
Komeda N, Chaudhary HK, Suzuki G, Mukai Y (2007) Cytological evidence for chromosome elimination in wheat × Imperata cylindrica hybrids. Genes Genet Syst 82:241–248. https://doi.org/10.1266/ggs.82.241
Krolow KD (1970) Investigations on compatibility between wheat and rye. Z Pflanzenzuchtung 64:44–72
Kwon YH, Kabange NR, Lee JY, Lee SM, Cha JK, Shin DJ et al (2021) Novel QTL associated with shoot branching identified in doubled haploid rice (Oryza sativa L.) under low nitrogen cultivation. Genes (Basel) 12:745. https://doi.org/10.3390/genes12050745
Lange W (1971) Crosses between Hordeum vulgare L. and H. bulbosum L. I. Production, morphology and meiosis of hybrids, haploids and dihaploids. Euphytica 20:14–29. https://doi.org/10.1007/BF00146769
Laurie DA (1989) The frequency of fertilization in wheat × pearl millet crosses. Genome 32:1063–1067. https://doi.org/10.1139/g89-554
Laurie DA, Bennett MD (1986) Wheat × maize hybridization. Can J Genet Cytol 28:313–316
Laurie DA, Bennett MD (1988a) Cytological evidence for fertilization in hexaploid wheat × sorghum crosses. Plant Breed 100:73–82
Laurie DA, Bennett MD (1988b) The production of haploid wheat plants from wheat × maize crosses. Theor Appl Genet 76:393–397. https://doi.org/10.1007/BF00265339
Laurie DA, Bennett MD (1989) The timing of chromosome elimination in hexaploid wheat × maize crosses. Genome 32:953–961. https://doi.org/10.1139/g89-537
Lazar MD, Schaffer GW, Baenziger PS (1985) The physical environment in relation to high frequency callus and plantlet development in anther cultures of wheat (Triticum aestivum L.) cv. Chris J Plant Physiol 121:103–109. https://doi.org/10.1016/S0176-1617(85)80034-1
Lee YT, Lim MS, Kim HS, Shin HT, Kim CH, Bae SH, Cho CI (1989) An anther derived new high-quality variety with disease and insect resistance ‘Hwacheong byeo’. Res Rep Rural Dev Admin Rice 31(2):27–23
Lee SY, Lee JH, Kwon TO (2003) Selection of salt-tolerant doubled haploids in rice anther culture. Plant Cell Tissue Organ Cult 74:143–149
Li L, Xu X, Jin W, Chen S (2009) Morphological and molecular evidences for DNA introgression in haploid induction via a high oil inducer CAUHOI in maize. Planta 230:367–376. https://doi.org/10.1007/s00425-009-0943-1
Li X, Meng D, Chen S et al (2017) Single nucleus sequencing reveals spermatid chromosome fragmentation as a possible cause of maize haploid induction. Nat Commun 8:991. https://doi.org/10.1038/s41467-017-00969-8
Liu X, Li R, Chang X, Jing R (2013) Mapping QTLs for seedling root traits in a doubled haploid wheat population under different water regimes. Euphytica 189:51–66. https://doi.org/10.1007/s10681-012-0690-4
Liu D, Zhang H, Zhang L, Yuan Z, Hao M, Zheng Y (2014) Distant hybridization: a tool for interspecific manipulation of chromosomes. In: Pratap A, Kumar J (eds) Alien gene transfer in crop plants, volume 1 innovations, methods and risk assessment. Springer, New York, pp 25–42. https://doi.org/10.1007/978-1-4614-8585-8_2
Liu C, Chen B, Ma Y et al (2017a) New insight into the mechanism of hetero fertilization during maize haploid induction. Euphytica 213:174. https://doi.org/10.1007/s10681-017-1957-6
Liu C, Li X, Meng D, Zhong Y, Chen C, Dong X, Xu X, Chen B, Li W, Li L, Tian X (2017b) A 4-bp insertion at ZmPLA1 encoding a putative phospholipase A generates haploid induction in maize. Mol Plant 10:520–522. https://doi.org/10.1016/j.molp.2017.01.011
Liu C, Zhong Y, Qi X, Chen M, Liu Z, Chen C, Tian X, Li J, Jiao Y, Wang D, Wang Y (2019) Extension of the in vivo haploid induction system from diploid maize to hexaploid wheat. Plant Biotechnol J 18:316–318. https://doi.org/10.1111/pbi.13218
Ma H, Li G, Würschum T, Zhang Y, Zheng D, Yang X, Li J, Liu W, Yan J, Chen S (2018) Genome-wide association study of haploid male fertility in maize (Zea mays L.). Front. Plant Sci 9:974. https://doi.org/10.3389/fpls.2018.00974
Mahato A, Chaudhary HK (2015) Relative efficiency of maize and Imperata cylindrica for haploid induction in Triticum durum following chromosomal elimination-mediated approach of doubled haploid breeding. Plant Breed 134:379–383. https://doi.org/10.1111/pbr.12288
Maluszynska J (2003) Cytogenetic tests for ploidy level analyses — chromosome counting. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1293-4_51
Maraschin SF, Caspers M, Potokina E, Wülfert F, Graner A, Spaink HP et al (2006) cDNA array analysis of stress-induced gene expression in barley androgenesis. Physiol Plant 127:535–550. https://doi.org/10.1111/j.1399-3054.2006.00673.x
Maruthachalam R, Chan SWL (2010) Haploid plants produced by centromere-mediated genome elimination. Nature 464:615–618. https://doi.org/10.1038/nature08842
McClinchey SL, Kott LS (2008) Production of mutants with high cold tolerance in spring canola (Brassica napus). Euphytica 162:51–67. https://doi.org/10.1007/s10681-007-9554-8
Mehta I, Chaudhary HK, Sharma P, Manoj NV, Singh K, Sran RS (2020) In vivo colchicine manipulation for enhancing DH production efficiency in Triticum durum using Imperata cylindrica- mediated chromosome elimination approach. Cereal Res Commun 48:217–224. https://doi.org/10.1007/s42976-020-00018-z
Melchinger AE, Schipprack W, Würschum T et al (2013) Rapid and accurate identification of in vivo-induced haploid seeds based on oil content in maize. Sci Rep 3:2129. https://doi.org/10.1038/srep02129
Melchinger AE, Schipprack W, Friedrich Utz H, Mirdita V (2014) In vivo haploid induction in maize: identification of haploid seeds by their oil content. Crop Sci 54:1497–1504. https://doi.org/10.2135/cropsci2013.12.0851
Melchinger AE, Böhm J, Utz HF et al (2018) High-throughput precision phenotyping of the oil content of single seeds of various oilseed crops. Crop Sci 58:670–678. https://doi.org/10.2135/cropsci2017.07.0429
Mishra R, Rao GJN (2016) In vitro androgenesis in rice: advantages, constraints and future prospects. Rice Sci 23:57–68. https://doi.org/10.1016/J.RSCI.2016.02.001
Mochida K, Tsujimoto H, Sasakuma T (2004) Confocal analysis of chromosome behaviour in wheat × maize zygotes. Genome 47:199–205. https://doi.org/10.1139/g03-123
Molenaar WS, Couto EGO, Piepho HP, Melchinger AE (2019a) Early diagnosis of ploidy status in doubled haploid production of maize by stomata length and flow cytometry measurements. Plant Breed 138:266–276. https://doi.org/10.1111/pbr.12694
Molenaar WS, Schipprack W, Brauner PC, Melchinger AE (2019b) Haploid male fertility and spontaneous chromosome doubling evaluated in a diallel and recurrent selection experiment in maize. Theor Appl Genet 132:2273–2284. https://doi.org/10.1007/s00122-019-03353-w
Mujeeb-Kazi A, Gul A, Ahmed J, Mirza JI (2006) A simplified and effective protocol for production of bread wheat haploids (n=3x=21, ABD) with some application areas in wheat improvement. Pak J Bot 38:393–406
Mujeeb-Kazi A, Gul A, Farooq M, Rizwan S, Ahmad I (2008) Rebirth of synthetic hexaploids with global implications for wheat improvement. Aust J Agric Res 59:391–398. https://doi.org/10.1071/AR07226
Murovec J, Bohanec B (2012) Haploids and doubled haploids in plant breeding. In: Abdurakhmonov I (ed) Plant breeding. INTECH, Rijeka. https://doi.org/10.5772/29982
Nair SK, Molenaar W, Melchinger AE et al (2017) Dissection of a major QTL qhir1 conferring maternal haploid induction ability in maize. Theor Appl Genet 130:1113–1122. https://doi.org/10.1007/s00122-017-2873-9
Nanda DK, Chase SS (1966) An embryo marker for detecting monoploids of maize (Zea mays L.). Crop Sci 6:213–215. https://doi.org/10.2135/cropsci1966.0011183X000600020036x
Niazian M, Shariatpanahi ME (2020) In vitro-based doubled haploid production: recent improvements. Euphytica 216:1–21. https://doi.org/10.1007/s10681-020-02609-7
Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87. https://doi.org/10.1126/science.163.3862.85
Niu Z, Jiang A, Abu Hammad W, Oladzadabbasabadi A, Xu SS, Mergoum M et al (2014) Review of doubled haploid production in durum and common wheat through wheat × maize hybridization. Plant Breed 133:313–320. https://doi.org/10.1111/pbr.12162
Ochatt SJ (2008) Flow cytometry in plant breeding. Cytometry A 73:581–598. https://doi.org/10.1002/cyto.a.20562
Ouyang JW, Hu H, Chuang CC, Tseng CC (1973) Induction of pollen plants from anthers of Triticum aestivum L. cultured in vitro. Sci Sinica 16:79–90. https://doi.org/10.1360/ya1973-16-1-79
Park GH, Kim JH, Kim KM (2014) QTL analysis of yield components in rice using a cheongcheong/nagdong doubled haploid genetic map. Am J Plant Sci 05:1174–1180. https://doi.org/10.4236/ajps.2014.59130
Patial M, Pal D, Thakur A, Bana RS, Patial S (2019) Doubled haploidy techniques in wheat (Triticum aestivum L.): an overview. Proc Natl Acad Sci India Sect B Biol Sci 89:27–41. https://doi.org/10.1007/s40011-017-0870-z
Patil VD, Nerkar YS, Misal MB, Harkal SR (1997) Parag 401, a semidwarf rice variety developed through anther culture. Int Rice Res Notes 22(2):19
Pauk J, Janeso M, Simon-Kiss I (2009) Rice doubled haploids and breeding A. In: Touraev BP, Forster SMJ (eds) Advances in haploid production in higher plants. Springer, Netherlands, pp 189–197
Pogna NE, Marzetti A (1977) Frequency of two tubes in in vitro germinated pollen grains. Maize Genet Coop News Lett 51:44
Prasanna BM (2012) Doubled haploid technology in maize breeding: an overview. In: Prasanna BM, Chaikam V, Mahuku G (eds) Doubled haploid technology in maize breeding: theory and practice. CIMMYT, Mexico, pp 1–8
Pratap A, Sethi GS, Chaudhary HK (2005) Relative efficiency of different Gramineae genera for haploid induction in triticale and triticale × wheat hybrids through chromosome elimination technique. Plant Breed 124:147–153. https://doi.org/10.1111/j.1439-0523.2004.01059.x
Pratap A, Sethi GS, Chaudhary HK (2006) Relative efficiency of anther culture and chromosome elimination technique for haploid induction in triticale × wheat and triticale × triticale hybrids. Euphytica 150:339–345. https://doi.org/10.1007/s10681-006-9120-9
Prigge V, Sánchez C, Dhillon BS, Melchinger AE (2011) Doubled haploids in tropical maize: I. Effects of inducers and source germplasm on in vivo haploid induction rates. Crop Sci 51:1498–1506. https://doi.org/10.2135/cropsci2010.10.0568
Prigge V, Schipprack W, Mahuku G et al (2012a) Development of in vivo haploid inducers for tropical maize breeding programs. Euphytica 185:481–490. https://doi.org/10.1007/s10681-012-0657-5
Prigge V, Xu X, Li L et al (2012b) New insights into the genetics of in vivo induction of maternal haploids, the backbone of doubled haploid technology in maize. Genetics 190:781–793. https://doi.org/10.1534/genetics.111.133066
Prins R, Pretorius ZA, Bender CM, Lehmensiek A (2011) QTL mapping of stripe, leaf and stem rust resistance genes in a Kariega × Avocet S doubled haploid wheat population. Mol Breed 27:259–270. https://doi.org/10.1007/s11032-010-9428-y
Qiu F, Liang Y, Li Y et al (2014) Morphological, cellular and molecular evidences of chromosome random elimination in vivo upon haploid induction in maize. Curr Plant Biol 1:83–90. https://doi.org/10.1016/j.cpb.2014.04.001
Qu Y, Liu Z, Zhang Y, Yang J, Li H (2021) Improving the sorting efficiency of maize haploid kernels using an NMR-based method with oil content double thresholds. Plant Methods 17:1–15. https://doi.org/10.1186/s13007-020-00703-4
Rahman MH, Krishnaraj S, Thorpe TA (1995) Selection for salt tolerance in vitro using microspore-derived embryos of Brassica napus cv Topas, and the characterization of putative tolerant plants. In Vitro Cell Dev Biol Plant 31:116–121. https://doi.org/10.1007/BF02632248
Rajcan I, Boersma JG, Shaw EJ (2011) Plant genetic techniques: plant breeder’s toolbox. Elsevier B.V, Second Edi. https://doi.org/10.1016/B978-0-08-088504-9.00252-X
Rakha MT, Metwally EI, Moustafa SA, Etman AA, Dewir YH (2012) Evaluation of regenerated strains from six Cucurbita interspecific hybrids obtained through anther and ovule in vitro cultures. Aust J Crop Sci 6:23–30
Ravi M, Chan SW (2010) Haploid plants produced by centromere-mediated genome elimination. Nature 464:615–618. https://doi.org/10.1038/nature08842
Ravi M, Marimuthu MP, Tan EH, Maheshwari S, Henry IM, Marin-Rodriguez B, Urtecho G, Tan J, Thornhill K, Zhu F, Panoli A (2014) A haploid genetics toolbox for Arabidopsis thaliana. Nat Commun 5:533–534. https://doi.org/10.1038/ncomms6334
Redha A, Talaat A (2008) Improvement of green plant regeneration by manipulation of anther culture induction medium of hexaploid wheat. Plant Cell Tissue Organ Cult 92:141–146. https://doi.org/10.1007/s11240-007-9315-3
Ren J, Wu P, Trampe B, Tian X, Lübberstedt T, Chen S (2017) Novel technologies in doubled haploid line development. Plant Biotechnol J 15:1361–1370. https://doi.org/10.1111/pbi.12805
Ren J, Boerman NA, Liu R, Vanous K, Trampe B, Frei UK, Chen S, Lübberstedt T (2019) Mapping of QTL and identification of candidate genes conferring spontaneous haploid genome doubling in maize (Zea mays L.). Plant Sci 293:110337. https://doi.org/10.1016/j.plantsci.2019.110337
Ribeiro CB, Pereira FC, Filho LN, Rezende BA, Dias KOG, Braz GT, Souza JC (2018) Haploid identification using tropicalized haploid inducer progenies in maize. Crop Breed Appl Biotechnol 18:16–23. https://doi.org/10.1590/1984-70332018v18n1a3
Riley R, Chapman V (1967a) Effect of 5BS in suppressing the expression of altered dosage of 5BL on meiotic chromosome pairing in Triticum aestivum. Nature 216:60–62. https://doi.org/10.1038/216060a0
Riley R, Chapman V (1967b) The inheritance in wheat of crossability with rye. Genet Res 9:259–267. https://doi.org/10.1017/S0016672300010569
Röber FK, Gordillo GA, Geiger HH (2005) In vivo haploid induction in maize-performance of new inducers and significance of doubled haploid lines in hybrid breeding. Maydica 50:275
Rotarenco VA, Kirtoca IH, Jacota AG (2007) Using oil content to identify kernels with haploid embryos. Maize Genet Coop News Lett 81:11
Rotarenco VA, Dicu G, State D, Fuia S (2010) New inducers of maternal haploids in maize. Maize Genet Coop News Lett 84:21–22
Samantaray S, Ali J, Nicolas KLC (2021) Doubled haploids in rice improvement: approaches, applications, and future prospects. In: Ali J, Wani SH (eds) Rice improvement: physiological, molecular breeding and genetic perspective. Springer, Cham, pp 425–447. https://doi.org/10.1007/978-3-030-66530-2
Sánchez-Díaz RA, Castillo AM, Vallés MP (2013) Microspore embryogenesis in wheat: new marker genes for early, middle and late stages of embryo development. Sex Plant Reprod 26:287–296. https://doi.org/10.1007/s00497-013-0225-8
Sarkar KR, Coe EH Jr (1971) Analysis of events leading to heterofertilization in maize. J Hered 62:118–120. https://doi.org/10.1093/oxfordjournals.jhered.a108136
Scagliusi SM (2014) Establishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.). Aust J Crop Sci 8:887–894
Scheeren LP, Caetano RV, Caierao E, Silva MS, Nascimento A, Eichelberger L, Miranda MZ, Brammer SP (2014) BRS 328 - Double haploid bread wheat cultivar. Crop Breed Appl Biotechnol 14:65–67. https://doi.org/10.1590/S1984-70332014000100011
Schwarzacher Robinson T, Finch RA, Smith JB, Bennett MD (1987) Genotypic control of centromere positions of parental genomes in Hordeum × Secale hybrid metaphases. J Cell Sci 87:291–304. https://doi.org/10.1242/jcs.87.2.291
Senadhira D, Zapata-Arias FJ, Gregorio GB, Alejar MS, De La Cruz HC, Padolina TF, Galvez AM (2002) Development of the first salt tolerant rice cultivar through indica/indica anther culture. Field Crops Res 76(2/3):103–110. https://doi.org/10.1016/S0378-4290(02)00032-1
Shariatpanahi ME, Ahmadi B (2016) Isolated microspore culture and its applications in plant breeding and genetics. In: Anis M, Ahmad N (eds) Plant tissue culture: propagation, conservation and crop improvement. Springer, Singapore. https://doi.org/10.1007/978-981-10-1917-3_21
Sharma P, Chaudhary HK, Manoj NV, Kumar P (2019a) New protocol for colchicine induced efficient doubled haploidy in haploid regenerants of tetraploid and hexaploid wheats at in vitro level. Cereal Res Commun 47:356–368. https://doi.org/10.1556/0806.47.2019.09
Sharma P, Chaudhary HK, Manoj NV, Singh K, Relan A, Sood VK (2019b) Haploid induction in triticale × wheat and wheat × rye derivatives following Imperata cylindrica- mediated chromosome elimination approach. Cereal Res Commun 47:701–713. https://doi.org/10.1556/0806.47.2019.46
Sidhu PK, Davies PA (2009) Regeneration of fertile green plants from oat isolated microspore culture. Plant Cell Rep 28:571–577. https://doi.org/10.1007/s00299-009-0684-4
Singh RB (1998) Agricultural biotechnology in Asia-Pacific region Agricultural Biotechnology in the Developing World. In: FAO Research & Technology Development Division. Daya Publishing House, New Delhi, pp 53–55
Sitch LA, Snape JW, Firman SJ (1985) Intra chromosomal mapping of crossability genes in wheat (Triticum aestivum). Theor Appl Genet 70:309–314. https://doi.org/10.1007/BF00304917
Snape JW, Simpson E, Parker BB et al (1986) Criteria for the selection and used doubled haploid systems in cereal breeding programmes. In: Horn W, Jensen CJ, Odenbach W, Shieder O (eds) Genetic manipulation in plant breeding. Walter de Gruyter, Berlin, pp 217–229
Sserumaga JP, Beyene Y, Pillay K, Kullaya A, Oikeh SO, Mugo S et al (2018) Grain-yield stability among tropical maize hybrids derived from doubled-haploid inbred lines under random drought stress and optimum moisture conditions. Crop Pasture Sci 69:691–702. https://doi.org/10.1071/CP17348
Strigens A, Schipprack W, Reif JC, Melchinger AE (2013) Unlocking the genetic diversity of maize landraces with doubled haploids opens new avenues for breeding. PLoS One 8:e57234. https://doi.org/10.1371/journal.pone.0057234
Subrahmanyam NC, Kasha KJ (1973) Selective chromosomal elimination during haploid formation in barley following interspecific hybridization. Chromosoma 42:111–125. https://doi.org/10.1007/BF00320934
Sugimoto K, Arai T (2002) Stability of characters of a doubled haploid rice variety, Shirayukihime. Breed Sci 52:15–21
Swapna M, Sarkar KR (2011) Anomalous fertilization in haploidy inducer lines in maize (Zea mays L). Maydica 56:221–225
Szarejko I, Forster BP (2007) Doubled haploidy and induced mutation. Euphytica 158:359–370. https://doi.org/10.1007/s10681-006-9241-1
Tang F, Tao Y, Zhao T, Wang G (2006) In vitro production of haploid and doubled haploid plants from pollinated ovaries of maize (Zea mays). Plant Cell Tissue Organ Cult 84:233–237. https://doi.org/10.1007/s11240-005-9017-7
Tefera AA (2017) Review on concept and impact of double haploid techniques in crop improvement. J Nat Sci Res 7:10–20. www.iiste.org
Thomas WTB, Forster BP, Gertsson B (2003) Doubled haploids in breeding. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1293-4_47
Tian X, Qin Y, Chen B et al (2018) Hetero-fertilization together with failed egg–sperm cell fusion supports single fertilization involved in in vivo haploid induction in maize. J Exp Bot 69(20):689–4701. https://doi.org/10.1093/jxb/ery177
Touraev A, Indrianto A, Wratschko I, Vicente O, Heberle BE (1996) Efficient microspore embryogenesis in wheat (Triticum aestivum L.) induced by starvation at high temperature. Sex Plant Reprod 9:209–215. https://doi.org/10.1007/BF02173100
Touraev A, Pfosser M, Heberle-Bors E (2001) The microspore: a haploid multipurpose cell. Adv Bot Res 35:53–109. https://doi.org/10.1016/S0065-2296(01)35004-8
Trampe B, dos Santos IG, Frei UK, Ren J, Chen S, Lübberstedt T (2020) QTL mapping of spontaneous haploid genome doubling using genotyping-by-sequencing in maize (Zea mays L.). Theor Appl Genet 133:2131–2140. https://doi.org/10.1007/s00122-020-03585-1
Tripathy SK, Swain D, Mohapatra PM, Prusti AM, Sahoo B, Panda S, Dash M, Chakma B, Behera SK (2019) Exploring factors affecting anther culture in rice (Oryza sativa L.). J Appl Biol Biotechnol 7:87–92. https://doi.org/10.7324/JABB.2019.70216
Tsuwamoto R, Fukuoka H, Takahata Y (2007) Identification and characterization of genes expressed in early embryogenesis from microspores of Brassica napus. Planta 225:641–652. https://doi.org/10.1007/s00425-006-0388-8
Vafadar Shamasbi F, Jamali SH, Sadeghzadeh B, Abdollahi Mandoulakani B (2017) Genetic mapping of quantitative trait loci for yield-affecting traits in a barley doubled haploid population derived from clipper × sahara 3771. Front Plant Sci 8:688. https://doi.org/10.3389/fpls.2017.00688
Vanous K, Vanous A, Frei UK, Lubberstedt T (2017) Generation of maize (Zea mays) doubled haploids via traditional methods. Curr Protoc Plant Biol 2:147–157. https://doi.org/10.1002/cppb.20050
Wang JL, Sun JS, Lu TG, Fang R, Cui HR, Cheng SZ, Yang C (1991) Fertilization and embryo development in wheat × maize crosses. Acta Bot Sin 33:674–679
Wang H, Liu J, Xu X et al (2016) Fully-automated high-throughput NMR system for screening of haploid kernels of maize (corn) by measurement of oil content. PLoS One 11:e0159444. https://doi.org/10.1371/journal.pone.0159444
Wang X-Y, Liao W-X, An D, Wei Y-G (2018) Maize haploid identification via LSTM-CNN and hyperspectral imaging technology. ArXiv, abs/1805.09105.
Wang S, Jin W, Wang K (2019) Centromere histone H3- and phospholipase-mediated haploid induction in plants. Plant Methods 15:42. https://doi.org/10.1186/s13007-019-0429-5
Wedzony M, Röber FK, Geiger HH (2002) Chromosome elimination observed in selfed progenies of maize inducer line RWS. In: XVIIth International Congress on sex plant reproduction. Maria Curie-Sklodowska University Press, Lublin
Wu P, Li H, Ren J, Chen S (2014) Mapping of maternal QTLs for in vivo haploid induction rate in maize (Zea mays L.). Euphytica 196:413–421. https://doi.org/10.1007/s10681-013-1043-7
Wu P, Ren J, Tian X et al (2017) New insights into the genetics of haploid male fertility in maize. Crop Sci 57:637–647
Xu Q, Yuan X, Yu H, Wang Y, Tang S, Wei X (2011) Mapping quantitative trait loci for sheath blight resistance in rice using double haploid population. Plant Breed 130:404–406. https://doi.org/10.1111/j.1439-0523.2010.01806.x
Xu X, Li L, Dong X et al (2013) Gametophytic and zygotic selection leads to segregation distortion through in vivo induction of a maternal haploid in maize. J Exp Bot 64:1083–1096. https://doi.org/10.1093/jxb/ers393
Yang XR, Fu HH (1989) Hua-03: a high protein indica rice. Intl Rice Res News Lett 14(3):14–15
Yao L, Zhang Y, Liu C, Liu Y, Wang Y, Liang D, Liu J, Sahoo G, Kelliher T (2018) OsMATL mutation induces haploid seed formation in indica rice. Nat Plants 4:530–533. https://doi.org/10.1038/s41477-018-0193-y
Ye JM, Kao KN, Harvey BL, Rossnagel BG (1987) Screening salt tolerant barley genotypes via F1 anther culture in salt stress media. Theor Appl Genet 74:426–429
Yu W, Birchler JA (2016) A green fluorescent protein-engineered haploid inducer line facilitates haploid mutant screens and doubled haploid breeding in maize. Mol Breed 36:1–12. https://doi.org/10.1007/s11032-015-0428-9
Yuan J, Guo X, Hu J, Lv Z, Han F (2015) Characterization of two CENH 3 genes and their roles in wheat evolution. New Phytol 206:839–851. https://doi.org/10.1111/nph.13235
Zenketler M, Straub J (1979) Cytoembryological study on the process of fertilization and the development of haploid embryo of Triticum aestivum (2n = 42) after crossing with Hordeum bulbosum (2n = 14). Z Pflanzenzuchtung 82:36–44
Zenkteler M, Nitzsche W (1984) Wide hybridization experiments in cereals. Theor Appl Genet 68:311–315. https://doi.org/10.1007/BF00267883
Zhahg-Yi Y, Hong-Ru K, Zhahg-Jin W, Li-Zheng Y, Zeng-Qian C (2008) High quality and blast resistance DH lines via anther culture. Southwest China J Agrl Sci 21:75–79
Zhang Z, Qiu F, Liu Y et al (2008) Chromosome elimination and in vivo haploid production induced by Stock 6-derived inducer line in maize (Zea mays L.). Plant Cell Rep 27:1851–1860. https://doi.org/10.1007/s00299-008-0601-2
Zhang W, Wang K, Lin ZS, Du LP, Ma HL, Xiao LL, Ye XG (2014) Production and identification of haploid dwarf male sterile wheat plants induced by corn inducer. Bot Stud 55:26. https://doi.org/10.1186/1999-3110-55-26
Zheng MY, Konzak CF (1999) Effect of 2,4-dichlorophenoxyacetic acid on callus induction and plant regeneration in anther culture of wheat (Triticum aestivum L.). Plant Cell Rep 19:69–73. https://doi.org/10.1007/s002990050712
Zheng YL, Luo MC, Yen C, Yang JL (1992) Chromosome location of a new crossability gene in common wheat. Wheat Inf Serv 75:36–40. https://doi.org/10.1534/genetics.109.107706
Zhong Y, Liu C, Qi X et al (2019) Mutation of ZmDMP enhances haploid induction in maize. Nat Plants 5:575–580. https://doi.org/10.1038/s41477-019-0443-7
Zhou H, Konzak CF (1989) Improvement of anther culture methods for haploid production in wheat. Crop Sci 29:817–821
Zhou C, Yang HY (1981) Induction of haploid rice plantlets by ovary culture. Plant Sci Lett 20:231–237. https://doi.org/10.1016/0304-4211(81)90267-4
Zhu ZC, Wu HS, An QK, Liu ZY (1981) Induction of haploid plantlets from unpollinated ovaries of Triticum aestivum cultured in vitro. Acta Genet Sin 8:586–590
Zhu T, Peterson DJ, Tagliani L, St. Clair G, Baszczynski CL, Brown B (1999) Targeted manipulation of maize genes in vivo using chimeric RNA/DNA oligonucleotides. Proceeding of National Academy of Sciences 96:8768–8773. https://doi.org/10.1073/pnas.96.15.8768
Żur I, Dubas E, Krzewska M, Sánchez-Díaz RA, Castillo AM, Vallés MP (2014) Changes in gene expression patterns associated with microspore embryogenesis in hexaploid triticale (Triticosecale Wittm.). Plant Cell Tissue Organ Cult 116:261–267. https://doi.org/10.1007/s11240-013-0399-7
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Sharma, D., Gahtyari, N.C., Sharma, P., Khulbe, R.K., Pal, R.S., Kant, L. (2022). Doubled Haploidy: An Accelerated Breeding Tool for Stress Resilience Breeding in Cereals. In: Gowdra Mallikarjuna, M., Nayaka, S.C., Kaul, T. (eds) Next-Generation Plant Breeding Approaches for Stress Resilience in Cereal Crops. Springer, Singapore. https://doi.org/10.1007/978-981-19-1445-4_6
Download citation
DOI: https://doi.org/10.1007/978-981-19-1445-4_6
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-19-1444-7
Online ISBN: 978-981-19-1445-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)