Abstract
Durum wheat × Imperata cylindrica hybridization was carried out utilizing two durum wheat genotypes as maternal parents (A-9-30-1 and PDW 314) and I. cylindrica as a pollen source followed by in vivo application of colchicine to establish a standard protocol for colchicine application aimed at enhancement of doubled haploid production in durum wheat. Various concentrations of colchicine ranging from 500 to 7000 ppm were injected to the pollinated spikes at different time interval viz., 24 h, 48 h and 72 h after pollination. Application of 5000–7000 ppm colchicine at 48 h after pollination was established as the optimum colchicine dose for chromosome doubling as confirmed through cytological studies in durum wheat using I. cylindrica-mediated chromosome elimination approach. PDW 314 was identified as a durum wheat genotype having more potential for doubled haploid induction via in vivo colchicine application than A-9-30-1. Identification of optimum dose of colchicine for application at in vivo level for induction of doubled haploids has opened new vistas in durum wheat improvement programme.
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Communicated by P. S. Baenziger.
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Figure S1
Relationship between colchicine application and pseudoseed formation frequency in A-9-30-1 (TIFF 20682 kb)
Figure S2
Relationship between colchicine application and pseudoseed formation frequency in PDW 314 (TIFF 21001 kb)
Figure S3
Relationship between colchicine application and embryo formation frequency in A-9-30-1 (TIFF 22156 kb)
Figure S4
Relationship between colchicine application and embryo formation frequency in PDW 314 (TIFF 21982 kb)
Figure S5
Relationship between colchicine application and embryo regeneration frequency in A-9-30-1 (TIFF 21739 kb)
Figure S6
Relationship between colchicine application and embryo regeneration frequency in PDW 314 (TIFF 21674 kb)
Figure S7
Relationship between colchicine application and plant formation efficiency in A-9-30-1 (TIFF 21832 kb)
Figure S8
Relationship between colchicine application and plant formation efficiency in PDW 314 (TIFF 21180 kb)
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Mehta, I., Chaudhary, H.K., Sharma, P. et al. In vivo colchicine manipulation for enhancing DH production efficiency in Triticum durum using Imperata cylindrica-mediated chromosome elimination approach. CEREAL RESEARCH COMMUNICATIONS 48, 217–224 (2020). https://doi.org/10.1007/s42976-020-00018-z
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DOI: https://doi.org/10.1007/s42976-020-00018-z