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Doubled haploidy and induced mutation

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Abstract

Doubled haploid (DH) systems have many attractive features for inducing and fixing mutations. Doubled haploidy provides the fastest route to homozygosity with the greatest fidelity. The ability to fix mutations via doubled haploidy is a key factor, especially as induced mutations␣are predominantly recessive and cannot normally be detected until the M2 generation at the earliest. The DH systems themselves provide an opportunity to target haploid as well as doubled haploid cells for mutation treatment and capture the mutation in a homozygous, pure line.

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Acknowledgments

The authors gratefully acknowledge Prof. M. Maluszynski for valuable comments and the critical review of this manuscript. The Scottish Crop Research Institute receives grant-in-aid from the Scottish Executive Environment and Rural Affairs Department.

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  1. Department of Genetics, University of Silesia, Jagiellonska 28, 40-032, Katowice, Poland

    I. Szarejko

  2. Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK

    B. P. Forster

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Szarejko, I., Forster, B.P. Doubled haploidy and induced mutation. Euphytica 158, 359–370 (2007). https://doi.org/10.1007/s10681-006-9241-1

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