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Cryopreservation of in vitro cultures of graminaceous species

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In Vitro Haploid Production in Higher Plants

Part of the book series: Current Plant Science and Biotechnology in Agriculture ((PSBA,volume 24))

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Abstract

The advent of in vitro techniques has supplemented conventional breeding methods. In the past, monocotyledonous species were considered to be recalcitrant in tissue culture. However, in the last decade, considerable progress has been made with the major cereal crops and pasture grasses, even though the initiation of tissue cultures continues to be strongly genotype-dependent. In most studies, immature embryo-, microspore- or anther-derived calli have been used to produce friable and embryogenic callus. These calli have typically been subcultured on solid media in order to select cell types suitable for the initiation of embryogenic cell suspensions. Currently, such cell lines are the only reliable source of totipotent protoplasts and provide promising targets for particle bombardment, and thereby, have become an excellent material for genetic transformation of cereals. However, the regeneration capacity of embryogenic cell lines has been found to decrease gradually during maintenance (Lührs and Lörz, 1988; Jähne et al., 1991; Datta et al., 1992). Therefore, it has been necessary to re-establish cell lines routinely. This is expensive and time consuming and incurs the risk of irretrievable loss through genetic change via somaclonal variation, microbial contamination or human error.

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Authors
  1. Andreas Fretz
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  2. Horst Lörz
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Editors and Affiliations

  1. Plant Production Department, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. School of Life Science, Jawaharlal Nehru University, New Delhi, India

    S. K. Sopory

  3. Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA

    R. E. Veilleux

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Fretz, A., Lörz, H. (1996). Cryopreservation of in vitro cultures of graminaceous species. In: Jain, S.M., Sopory, S.K., Veilleux, R.E. (eds) In Vitro Haploid Production in Higher Plants. Current Plant Science and Biotechnology in Agriculture, vol 24. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0477-9_16

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  • DOI: https://doi.org/10.1007/978-94-017-0477-9_16

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