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Initiation of morphogenic cell-suspension and protoplast cultures of barley (Hordeum vulgare L.)

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Abstract

Cell-suspension cultures were initiated from embryogenic calli of various barley cultivars. Seven fast-growing suspension lines were obtained from four different cultivars (cvs. Dissa, Emir, Golden Promise and Igri). Two of these cell suspensions showed morphogenic capacity. From a cell suspension of cv. Dissa, albino plantlets were regenerated when aggregates were cultured on solid medium. Aggregates of cv. Igri usually stopped differentiation at the globular stage, but occasionally formed scutellum-like structures. Five suspension lines were used for protoplast isolation and culture. Dividing protoplasts were obtained from all lines, but with cv. Igri a few divisions only and no further development were observed. Protoplasts from the various lines differed in the time of first division (2–14 d), division frequency (0.09–70.9%) and efficiency of colony formation (0.09–7.3%). Protoplasts isolated from the morphogenic cell suspension of cv. Dissa developed compact calli which sporadically regenerated albino plantlets.

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Abbreviations

CC, MS, N6, SH, Kao8p:

culture media; see Material and methods

cv:

chltivar

dicamba:

3,6-dichloro-o-anisic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

picloram:

4-amino-3,5,6-trichloropicolinic acid

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Authors and Affiliations

  1. Max-Planck-Institut für Züchtungsforschung, D-5000, Köln 30 (Vogelsang), Germany

    Renate Lührs & Horst Lörz

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  1. Renate Lührs
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  2. Horst Lörz
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Lührs, R., Lörz, H. Initiation of morphogenic cell-suspension and protoplast cultures of barley (Hordeum vulgare L.). Planta 175, 71–81 (1988). https://doi.org/10.1007/BF00402883

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  • DOI: https://doi.org/10.1007/BF00402883

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