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The regeneration of plants from frozen pollen embryos and zygotic embryos of wheat and rice

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Summary

Anther culture derived pollen embryos and immature zygotic embryos of wheat and rice, frozen in liquid nitrogen in the presence of dimethyl sulfoxide, sucrose and glycerol, have been revived. The retrieved cultures proliferated and/or regenerated shoots and plantlets. The prospects of the cryopreservation of embryos for the conservation and multiplication of germplasm and the possibility of the establishment of ‘Germplasm Banks’ are discussed.

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Authors and Affiliations

  1. Tissue Culture Laboratory, Punjab Agricultural University, Ludhiana, India

    Y. P. S. Bajaj

Authors
  1. Y. P. S. Bajaj
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Communicated by Hu Han

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Bajaj, Y.P.S. The regeneration of plants from frozen pollen embryos and zygotic embryos of wheat and rice. Theoret. Appl. Genetics 67, 525–528 (1984). https://doi.org/10.1007/BF00264898

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  • DOI: https://doi.org/10.1007/BF00264898

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