Skip to main content
Log in

Medium-term conservation of mature embryos of coconut

  • Original Papers
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Optimal conditions for the medium-term conservation of zygotic embryos of coconut were developed. After 6 months of storage on a medium devoid of sucrose and containing 2 g l−1 activated charcoal, 100% of the embryos developed into whole plantlets within 5 months after transfer to the recovery medium. After a 12-month storage period on medium containing 15 g l−1 sucrose and devoid of activated charcoal, 51% of the embryos germinated within 2 months after transfer to the recovery medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Arumugam N & Bhojwani SS (1990)In vitro propagation ofPodophyllum hexandrum Boyle via somatic embryogenesis. In: Abst. VIIth Int. Cong. Plant Tissue and Cell Culture, Amsterdam (p 243)

  • Assy-Bah B (1986) Culturein vitro d'embryons zygotiques de cocotiers. Oléagineux 41: 321–328

    Google Scholar 

  • Assy-Bah B, Durand-Gasselin T & Pannetier C (1987) Use of zygotic embryo culture to collect germplasm of coconut (Cocos nucifera L.). FAO/IBPGR Plant Genet. Res. Newsl. 71: 4–10

    Google Scholar 

  • Assy-Bah B, Durand-Gasselin T, Engelmann F & Pannetier C (1989) Culturein vitro d'embryons zygotiques de cocotier (Cocos nucifera L.). Méthode, révisée et simplifiée, d'obtention de plants transférables aux champs. Oléagineux 44: 515–523

    Google Scholar 

  • Assy-Bah B & Engelmann F (1992a) Cryopreservation of immature embryos of coconut (Cocos nucifera L.). Cryo-Letters 13: 67–74

    Google Scholar 

  • Assy-Bah B & Engelmann F (1992b) Cryopreservation of mature embryos of coconut (Cocos nucifera L.) and subsequent regeneration of plantlets. Cryo-Letters 13: 117–126

    Google Scholar 

  • Banerjee N & De Langhe EA (1985) A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions ofMusa (banana and plantain). Plant Cell Rep. 4: 351–354

    Article  Google Scholar 

  • Bapat VA & Rao PS (1988) Sandalwood plantlets from ‘synthetic seeds’. Plant Cell Rep. 7: 434–436

    Article  Google Scholar 

  • De Guzman EV, Del Rosario AG & Eusebio EC (1971) The growth and development of coconut ‘Makapuno’ embryoin vitro. Resumption of growth in high sugar media. Philipp. Agri. 53: 566–579

    Google Scholar 

  • Ebert A & Taylor HF (1990) Assessment of the changes of 2,4-dichlorophenoxyacetic acid concentration in plant tissue culture media in the presence of activated charcoal. Plant Cell Tiss. Org. Cult. 20: 165–172

    Google Scholar 

  • Engelmann F (1990) Utilisation d'atmosphères à teneur en oxygène réduite pour la conservation de cultures d'embryons somatiques de palmier à huile (Elaeis guineensis Jacq.). C.R. Acad. Sci. Paris 310, Sér. III: 679–684

  • Engelmann F (1991)In vitro conservation of tropical plant germplasm — a review. Euphytica 57: 227–243

    Article  Google Scholar 

  • Frison EA & Putter CAJ (1992) FAO/IBPGR Technical Guidelines for the Safe Movement of Germplasm. Food and Agriculture Organization of the United Nations, Rome/International Board for Plant Genetic Resources, Rome (in press)

  • Gray DJ (1989) Effects of dehydration and exogenous growth regulators on dormancy, quiescence and germination of grape somatic embryos.In Vitro Cell Dev. Biol. 25: 1173–1178

    Google Scholar 

  • Karunaratne S, Kurukulaarachchi C & Gamage C (1985) A report on the culture of dwarf coconut.Cocos nucifera L. var.nana in vitro. Cocos 3: 1–8

    Google Scholar 

  • Morel G & Wetmore RH (1951) Tissue culture of monocotyledons. Am. J. Bot. 38: 138–140

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Pierik RLM (1987)In Vitro Culture of Higher Plants. Martinus Nijhoff, Dordrecht

    Google Scholar 

  • Rillo EP & Paloma BF (1990) Comparison of three media formulations forin vitro culture of coconut embryos. Oléagineux 45: 319–323

    Google Scholar 

  • Withers LA (1991) Tissue culture in the conservation of plant genetic resources. In: Zakri AH, Normah MN, Senawi MT & Abdul Karim AG (Eds) Conservation of plant genetic resources throughin vitro methods (pp 1–18). Forest Research Institute Malaysia/Malaysian National Committee on Plant Genetic Resources, Kuala Lumpur, Malaysia

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Assy-Bah, B., Engelmann, F. Medium-term conservation of mature embryos of coconut. Plant Cell Tiss Organ Cult 33, 19–24 (1993). https://doi.org/10.1007/BF01997593

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01997593

Key words

Navigation