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Cryopreservation of wheat suspension culture and regenerable callus

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Abstract

Wheat (Triticum aestivum L. cv. Norstar) suspension cultures and regenerable calli initiated from immature embryos can be cryopreserved in liquid nitrogen temperature (−196°C) by slow freezing (0.5°C/min) in the presence of a mixture of DMSO and sucrose or sorbitol. Cold hardening or ABA treatment before cryopreservation increased the freezing resistance and improved the survival of wheat suspension culture in liquid nitrogen. Callus culture, established from immature embryos, prefrozen in 5% DMSO and 0.5M sorbitol survived liquid nitrogen storage and resumed plant regeneration after thawing. The results confirm the feasibility of long term preservation of wheat embryo callus by cryopreservation and retention of plant regeneration ability.

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Abbreviations

ABA:

Abscisic acid

2,4-D:

2,4-Dichlorophenoxyacetic acid

DMSO:

Dimethylsulfoxide

LN:

Liquid nitrogen

TTC:

2,3,5-triphenyltetrazolium chloride

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Author notes

  1. Tony H. H. Chen

    Present address: Alberta Research Council, 11315-87th Avenue, T6G 2C2, Edmonton, Alberta

  2. Lawrence V. Gusta

    Present address: Crop Development Centre, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada

Authors and Affiliations

  1. Plant Biotechnology Institute, National Research Council, S7N 0W9, Saskatoon, Saskatchewan, Canada

    Tony H. H. Chen, Kutty K. Kartha & Lawrence V. Gusta

Authors
  1. Tony H. H. Chen
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  2. Kutty K. Kartha
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  3. Lawrence V. Gusta
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Additional information

NRCC No. 23850.

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Chen, T.H.H., Kartha, K.K. & Gusta, L.V. Cryopreservation of wheat suspension culture and regenerable callus. Plant Cell Tiss Organ Cult 4, 101–109 (1985). https://doi.org/10.1007/BF00042268

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  • DOI: https://doi.org/10.1007/BF00042268

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