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Plant regeneration from grape callus stored under a combination of low temperature and silicone treatment

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Abstract

Effects of the combination of low temperature and silicone treatment on the storage of grape callus (Vitis vinifera L. x V. labrusca L. cv. Kyoho; V. vinifera L. cv. Koshusanjaku) were examined. In ‘Kyoho’, the calli were stored at 10°C successfully for up to 360 days. Embryogenic calli of ‘Koshusanjaku’ stored at 10°C retained the ability of embryogenesis after 360 days of storage. However, the color of both calli became brownish. This was improved by the combination of low temperature and silicone treatment. The calli of ‘Kyoho’ survived by the storage under the combination of 15°C and silicone. Embryogenic calli stored at 10 and 15°C in combination with silicone survived for 360 days, and regenerated only after transfer onto a regeneration medium. Thus the combination of low temperature and silicone affects the longevity of the grape callus.

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Author notes

  1. S. Yamaki

    Present address: Faculty of Agriculture Nagoya University, Chikusa, 464, Nagoya, Japan

Authors and Affiliations

  1. Fruit Tree Research Station, Ministry of Agriculture, Forestry and Fisheries, 305, Tsukuba, Ibaraki, Japan

    T. Moriguchi, I. Kozaki, N. Matsuta & S. Yamaki

Authors
  1. T. Moriguchi
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  2. I. Kozaki
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  3. N. Matsuta
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  4. S. Yamaki
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Moriguchi, T., Kozaki, I., Matsuta, N. et al. Plant regeneration from grape callus stored under a combination of low temperature and silicone treatment. Plant Cell Tiss Organ Cult 15, 67–71 (1988). https://doi.org/10.1007/BF00039890

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  • DOI: https://doi.org/10.1007/BF00039890

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