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Difference Gel Electrophoresis

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2596)

Buying options

Hardcover Book USD 249.99
Price excludes VAT (USA)
This title has not yet been released. You will be able to pre-order it soon.

About this book

The second edition of this volume provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

 

 

Keywords

  • 2D-DIGE
  • DIGE-Based
  • post-translational modifications
  • Glycoproteomic Analysis
  • Ffpe

Editors and Affiliations

  • Maynooth University, MAYNOOTH, Ireland

    Kay Ohlendieck

Bibliographic Information

  • Book Title: Difference Gel Electrophoresis

  • Book Subtitle: Methods and Protocols

  • Editors: Kay Ohlendieck

  • Series Title: Methods in Molecular Biology

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XX, 480

  • Number of Illustrations: 8 b/w illustrations, 74 illustrations in colour

  • Topics: Proteins

Buying options

Hardcover Book USD 249.99
Price excludes VAT (USA)
This title has not yet been released. You will be able to pre-order it soon.