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Yeast Metabolic Engineering

Methods and Protocols

  • Book
  • © 2022
  • Latest edition

Overview

Editors:
  1. Valeria Mapelli

    1. Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden

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  2. Maurizio Bettiga

    1. Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2513)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
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  2. An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae

    • Verena Siewers
    Pages 1-13

  3. High-Efficiency Plasmid DNA Transformation in Yeast

    • O’Taveon R. Fitzgerald, Nestor D. Rodriguez, L. Kevin Lewis
    Pages 15-22

  4. CasPER: A CRISPR/Cas9-Based Method for Directed Evolution in Genomic Loci in Saccharomyces cerevisiae

    • Tadas Jakočiūnas, Michael K. Jensen, Jay D. Keasling
    Pages 23-37

  5. Fluorescence-Activated Cell Sorting as a Tool for Recombinant Strain Screening

    • Christos Skrekas, Raphael Ferreira, Florian David
    Pages 39-57

  6. Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology

    • Kouichi Kuroda, Mitsuyoshi Ueda
    Pages 59-77

  7. Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris

    • Claudia Rinnofner, Michael Felber, Harald Pichler
    Pages 79-112

  8. Competent Cell Preparation and Transformation of Pichia pastoris

    • Joan Lin-Cereghino, Christopher A. Naranjo, Geoff P. Lin-Cereghino
    Pages 113-120

  9. CRISPR/Cas9 Tool Kit for Efficient and Targeted Insertion/Deletion Mutagenesis of the Komagataella phaffii (Pichia pastoris) Genome

    • Jasmin Elgin Fischer, Anton Glieder
    Pages 121-133

  10. Plasmid-Based Gene Knockout Strategy with Subsequent Marker Recycling in Pichia pastoris

    • Simon Kobalter, Astrid Radkohl, Helmut Schwab, Anita Emmerstorfer-Augustin, Harald Pichler
    Pages 135-151

  11. Engineering of Promoters for Gene Expression in Pichia pastoris

    • Thomas Vogl
    Pages 153-177

  12. Molecular Tools for Leveraging the Potential of the Acid-Tolerant Yeast Zygosaccharomyces bailii as Cell Factory

    • Paola Branduardi, Liliane Barroso, Laura Dato, Edward J. Louis, Danilo Porro
    Pages 179-204

  13. Golden Gate Multigene Assembly Method for Yarrowia lipolytica

    • Macarena Larroude, Jean-Marc Nicaud, Tristan Rossignol
    Pages 205-220

  14. A Universal Gene Expression System for Novel Yeast Species

    • Dominik Mojzita, Anssi Rantasalo, Marja Ilmén
    Pages 221-242

  15. Dynamic Feeding for Pichia pastoris

    • Alexander Pekarsky, Oliver Spadiut
    Pages 243-254

  16. Automated Evolutionary Engineering of Yeasts

    • Erik de Hulster, Christiaan Mooiman, Rowin Timmermans, Robert Mans
    Pages 255-270

  17. Reconstruction of Genome-Scale Metabolic Model for Hansenula polymorpha Using RAVEN

    • Francisco Zorrilla, Eduard J. Kerkhoven
    Pages 271-290

  18. Back Matter

    Pages 291-295
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Keywords

About this book

This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.





Editors and Affiliations

  • Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden

    Valeria Mapelli, Maurizio Bettiga

Bibliographic Information

  • Book Title: Yeast Metabolic Engineering

  • Book Subtitle: Methods and Protocols

  • Editors: Valeria Mapelli, Maurizio Bettiga

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2399-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2398-5Published: 04 July 2022

  • Softcover ISBN: 978-1-0716-2401-2Published: 04 July 2022

  • eBook ISBN: 978-1-0716-2399-2Published: 04 July 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 295

  • Number of Illustrations: 19 b/w illustrations, 32 illustrations in colour

  • Topics: Biotechnology, Analytical Chemistry, Materials Science, general

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