Abstract
The current progress in sequencing of genomes and characterization of new species provides an increasing list of yeasts that show interesting physiological properties; however, the lack of expression tools for these new hosts is prohibiting their broader use in research or industry. Recently, we developed a universal expression system (SES) functional in broad spectrum of fungal species, which represent a solution for feasible gene expression control and genetic manipulation in these novel hosts. Here, we describe three example approaches for DNA transformation and high-level heterologous gene expression, using the SES system, in three yeast species, where minimal knowledge or prior experience in genetic modifications is available.
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Acknowledgments
We thank Dr. Jussi Jäntti and Dr. Christopher Landowski for critical reading of the manuscript. This material is based upon work supported by the Finnish Funding Agency for Innovation (living factories project), and the Academy of Finland (MODUBIO, project 260957; SynBioPol, project 272550). Synthetic expression system (SES) has been granted a patent (Finnish patent (FI127283B) March 15, 2018) and is the subject of patent applications (PCT/FI2017/050114 on February 21, 2017 and 015234 US on December 18, 2017).
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Mojzita, D., Rantasalo, A., Ilmén, M. (2022). A Universal Gene Expression System for Novel Yeast Species. In: Mapelli, V., Bettiga, M. (eds) Yeast Metabolic Engineering. Methods in Molecular Biology, vol 2513. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2399-2_13
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DOI: https://doi.org/10.1007/978-1-0716-2399-2_13
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