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Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology

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Yeast Metabolic Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2513))

Abstract

The cell surface display system in yeast enables the innovative strategy for improving cellular functions in a wide range of applications such as biofuel production, bioremediation, synthesis of valuable chemicals, recovery of rare metal ions, development of biosensors, and high-throughput screening of protein/peptide library. Display of enzymes for polysaccharide degradation enables the construction of metabolically engineered whole-cell biocatalyst owing to the accessibility of the displayed enzymes to high-molecular-weight polysaccharides. In addition, along with fluorescence-based activity evaluation, fluorescence-activated cell sorting (FACS), and yeast cell chip, the cell surface display system is an effective molecular tool for high-throughput screening of mutated protein/peptide library. In this article, we describe the methods for cell surface display of proteins/peptides of interest on yeast, evaluation of display efficiency, and harvesting of the displayed proteins/peptides from cell surface.

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Authors and Affiliations

  1. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan

    Kouichi Kuroda & Mitsuyoshi Ueda

Authors
  1. Kouichi Kuroda
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  2. Mitsuyoshi Ueda
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Correspondence to Mitsuyoshi Ueda .

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Editors and Affiliations

  1. Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden

    Valeria Mapelli

  2. Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden

    Maurizio Bettiga

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Kuroda, K., Ueda, M. (2022). Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology. In: Mapelli, V., Bettiga, M. (eds) Yeast Metabolic Engineering. Methods in Molecular Biology, vol 2513. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2399-2_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2399-2_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2398-5

  • Online ISBN: 978-1-0716-2399-2

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