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  • Open Access
  • © 2022


Methods and Protocols

  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts

  • This book is open access, which means that you have free and unlimited access

Part of the book series: Methods in Molecular Biology (MIMB, volume 2453)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xvi
  2. Next-Generation Sequencing-Based Clonality Detection of Immunoglobulin Gene Rearrangements in B-Cell Lymphoma

    • Diede A. G. van Bladel, Jessica L. M. van der Last-Kempkes, Blanca Scheijen, Patricia J. T. A. Groenen, on behalf of the EuroClonality Consortium
    Pages 7-42Open Access
  3. One-Step Next-Generation Sequencing of Immunoglobulin and T-Cell Receptor Gene Recombinations for MRD Marker Identification in Acute Lymphoblastic Leukemia

    • Patrick Villarese, Chrystelle Abdo, Matthieu Bertrand, Florian Thonier, Mathieu Giraud, Mikaël Salson et al.
    Pages 43-59Open Access
  4. Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq

    • Vincent H. J. van der Velden, Lorenz Bastian, Monika Brüggemann, Alina M. Hartmann, Nikos Darzentas
    Pages 61-77Open Access
  5. Minimal Residual Disease Analysis by Monitoring Immunoglobulin and T-Cell Receptor Gene Rearrangements by Quantitative PCR and Droplet Digital PCR

    • Irene Della Starza, Cornelia Eckert, Daniela Drandi, Giovanni Cazzaniga, on behalf of the EuroMRD Consortium
    Pages 79-89Open Access
  6. Quality Control for IG /TR Marker Identification and MRD Analysis

    • Eva Fronkova, Michael Svaton, Jan Trka
    Pages 91-99Open Access
  7. cfDNA-Based NGS IG Analysis in Lymphoma

    • Christiane Pott, Michaela Kotrova, Nikos Darzentas, Monika Brüggemann, Mouhamad Khouja, on behalf of the EuroClonality-NGS Working Group
    Pages 101-117Open Access
  8. Targeted Locus Amplification as Marker Screening Approach to Detect Immunoglobulin (IG) Translocations in B-Cell Non-Hodgkin Lymphomas

    • Elisa Genuardi, Beatrice Alessandria, Aurora Maria Civita, Simone Ferrero
    Pages 119-132Open Access
  9. Immunoglobulin/T Cell Receptor Capture Strategy for Comprehensive Immunogenetics

    • James Peter Stewart, Jana Gazdova, Shambhavi Srivastava, Julia Revolta, Louise Harewood, Manisha Maurya et al.
    Pages 133-152Open Access
  10. Immunoglobulin Gene Mutational Status Assessment by Next Generation Sequencing in Chronic Lymphocytic Leukemia

    • Anne Langlois de Septenville, Myriam Boudjoghra, Clotilde Bravetti, Marine Armand, Mikaël Salson, Mathieu Giraud et al.
    Pages 153-167Open Access
  11. NGS-Based B-Cell Receptor Repertoire AnalysisRepertoire analyses in the Context of Inborn Errors of Immunity

    • Pauline A. van Schouwenburg, Mirjam van der Burg, Hanna IJspeert
    Pages 169-190Open Access
  12. Generic Multiplex Digital PCR for Accurate Quantification of T Cells in Copy Number Stable and Unstable DNA Samples

    • Rogier J. Nell, Willem H. Zoutman, Mieke Versluis, Pieter A. van der Velden
    Pages 191-208Open Access
  13. Gene Engineering T Cells with T-Cell Receptor for Adoptive Therapy

    • Dian Kortleve, Mandy van Brakel, Rebecca Wijers, Reno Debets, Dora Hammerl
    Pages 209-229Open Access
  14. Combined Analysis of Transcriptome and T-Cell Receptor Alpha and Beta (TRA /TRB ) Repertoire in Paucicellular Samples at the Single-Cell Level

    • Nicolle H. R. Litjens, Anton W. Langerak, Zakia Azmani, Xander den Dekker, Michiel G. H. Betjes, Rutger W. W. Brouwer et al.
    Pages 231-259Open Access
  15. AIRR Community Guide to Planning and Performing AIRR-Seq Experiments

    • Anne Eugster, Magnolia L. Bostick, Nidhi Gupta, Encarnita Mariotti-Ferrandiz, Gloria Kraus, Wenzhao Meng et al.
    Pages 261-278Open Access
  16. Adaptive Immune Receptor Repertoire (AIRR) Community Guide to TR and IG Gene Annotation

    • Lmar Babrak, Susanna Marquez, Christian E. Busse, William D. Lees, Enkelejda Miho, Mats Ohlin et al.
    Pages 279-296Open Access
  17. Adaptive Immune Receptor Repertoire (AIRR) Community Guide to Repertoire Analysis

    • Susanna Marquez, Lmar Babrak, Victor Greiff, Kenneth B. Hoehn, William D. Lees, Eline T. Luning Prak et al.
    Pages 297-316Open Access
  18. Bulk gDNA Sequencing of Antibody Heavy-Chain Gene Rearrangements for Detection and Analysis of B-Cell Clone Distribution: A Method by the AIRR Community

    • Aaron M. Rosenfeld, Wenzhao Meng, Kalisse I. Horne, Elaine C. Chen, Davide Bagnara, Ulrik Stervbo et al.
    Pages 317-343Open Access
  19. Bulk Sequencing from mRNA with UMI for Evaluation of B-Cell Isotype and Clonal Evolution: A Method by the AIRR Community

    • Nidhi Gupta, Susanna Marquez, Cinque Soto, Elaine C. Chen, Magnolia L. Bostick, Ulrik Stervbo et al.
    Pages 345-377Open Access

About this book

This open access book explores techniques for working in the field of immunogenetics, i.e. fundamental and translational research into the adaptive immune receptor repertoire. Many chapters are dedicated to lab protocols, bioinformatics, and immunoinformatics analysis of high-resolution immunome analysis, exemplified by numerous applications. Additionally, the newest technological variations on these protocols are discussed, including non-amplicon, single-cell, and cell-free strategies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Immunogenetics: Methods and Protocols covers a broad spectrum of methodologies for applications in research and clinical diagnostics to illustrate the impact that immunogenetics has achieved and will further expand in all fields of medicine, from infection and (auto)immunity, to vaccination, to lymphoid malignancy and tumor immunity.

Editors and Affiliations

  • Department of Immunology, Erasmus MC, Rotterdam, The Netherlands

    Anton W. Langerak

Bibliographic Information

  • Book Title: Immunogenetics

  • Book Subtitle: Methods and Protocols

  • Editors: Anton W. Langerak

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s) 2022

  • License: CC BY

  • Hardcover ISBN: 978-1-0716-2114-1Published: 28 May 2022

  • Softcover ISBN: 978-1-0716-2117-2Published: 18 January 2023

  • eBook ISBN: 978-1-0716-2115-8Published: 27 May 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 607

  • Number of Illustrations: 11 b/w illustrations, 121 illustrations in colour

  • Topics: Immunology

Buy it now

Buying options

Hardcover Book USD 59.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access