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Polycyclic aromatic hydrocarbons degradation by free-air CO2 enriched (FACE) bacteria into low molecular easy to degrade organic compounds

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Abstract

Among the 16 prioritized polycyclic aromatic hydrocarbons (PAHs), naphthalene, phenanthrene, fluoranthene, and pyrene have been used for bacterial degradation study. From the free-air CO2 enriched (FACE) soil, five Bacillus strains were isolated and used to utilize the four model toxicants at different concentrations as the sole carbon source. Bacillus amyloliquefaciens has great resistance to different PAHs and better degradation capability. B. amyloliquefaciens can degrade naphthalene (0.5 mg mL− 1), fluoranthene (0.1 mg mL− 1), and pyrene (0.1 mg mL− 1) up to 94%, 65%, and 56% respectively, while B. cereus mineralized phenanthrene (0.5 mg mL− 1) up to 71% within seven days of incubation. B amyloliquefaciens and B. cereus have the capability of ring cleavage and they can convert PAH compounds into less toxic compounds. Based on the metabolites obtained through GC-MS, the biodegradation pathways for each PAH have been predicted to end up in the tricarboxylic acid cycle.

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Acknowledgements

We would like to thank Advanced Instrumentation Research Facility (AIRF) Jawaharlal Nehru University, New Delhi for GC–MS analysis.

Funding

Experimental cost was funded by DST-SERB, Government of India, New Delhi and CSIR-New Delhi, India.

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Authors and Affiliations

  1. Amity School of Earth and Environmental Sciences, Amity University Gurgaon, Haryana, 122413, India

    Vivek Manyapu, Neha Maheshwari, Indu Shekhar Thakur & Shaili Srivastava

  2. Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan

    Vivek Manyapu

  3. CSIR-Central Drug Research Institute (CDRI), Lucknow, 226031, Uttar Pradesh, India

    Neha Maheshwari

  4. University of Freiburg, Freiburg im Breisgau, Freiburg im Breisgau, 79085, Germany

    Arti Sharma

  5. School of Environmental Science, Jawaharlal Nehru University, New Delhi, 110067, India

    Indu Shekhar Thakur & Shaili Srivastava

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Contributions

Experiments were designed by SS. VM and NM performed all the experiments under the guidance of SS. Manuscript was written by VM and edited by SS. AS designed the chemical structures and pathways. At last, all authors revised and finalized the manuscript for submission.

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Correspondence to Shaili Srivastava.

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Manyapu, V., Maheshwari, N., Sharma, A. et al. Polycyclic aromatic hydrocarbons degradation by free-air CO2 enriched (FACE) bacteria into low molecular easy to degrade organic compounds. Syst Microbiol and Biomanuf (2024). https://doi.org/10.1007/s43393-024-00259-7

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