Abstract
Xylanases from thermophilic fungi have a wide range of commercial applications in the bioconversion of lignocellulosic materials and biobleaching in the pulp and paper industry. In this study, an endoxylanase from the thermophilic fungus Rasamsonia composticola (XylRc) was produced using waste wheat bran and pretreated sugarcane bagasse (PSB) in solid-state fermentation. The enzyme was purified, biochemically characterized, and used for the saccharification of sugarcane bagasse. XylRc was purified 30.6-fold with a 22% yield. The analysis using sodium dodecyl sulphate–polyacrylamide gel electrophoresis revealed a molecular weight of 53 kDa, with optimal temperature and pH values of 80 °C and 5.5, respectively. Thin-layer chromatography suggests that the enzyme is an endoxylanase and belongs to the glycoside hydrolase 10 family. The enzyme was stimulated by the presence of K+, Ca2+, Mg2+, and Co2+ and remained stable in the presence of the surfactant Triton X-100. XylRc was also stimulated by organic solvents butanol (113%), ethanol (175%), isopropanol (176%), and acetone (185%). The Km and Vmax values for oat spelt and birchwood xylan were 6.7 ± 0.7 mg/mL, 2.3 ± 0.59 mg/mL, 446.7 ± 12.7 µmol/min/mg, and 173.7 ± 6.5 µmol/min/mg, respectively. XylRc was unaffected by different phenolic compounds: ferulic, tannic, cinnamic, benzoic, and coumaric acids at concentrations of 2.5–10 mg/mL. The results of saccharification of PSB showed that supplementation of a commercial enzymatic cocktail (Cellic® CTec2) with XylRc (1:1 w/v) led to an increase in the degree of synergism (DS) in total reducing sugar (1.28) and glucose released (1.05) compared to the control (Cellic® HTec2). In summary, XylRc demonstrated significant potential for applications in lignocellulosic biomass hydrolysis, making it an attractive alternative for producing xylooligosaccharides and xylose, which can serve as precursors for biofuel production.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Data availability
The data are available from the corresponding author on reasonable request.
References
Ajao O, Marinova M, Savadogo O, Paris JJIC, Products (2018) Hemicellulose based integrated forest biorefineries: Implementation strategies. Ind Crops Prod 126:250–260. https://doi.org/10.1016/j.indcrop.2018.10.025
Almeida AP, Vargas IP, Marciano CL, Zanoelo FF, Giannesi GC, Polizeli MLTM, Jorge JA, Furriel RPM, Ruller R, Masui DC (2022) Investigation of biochemical and biotechnological potential of a thermo-halo-alkali-tolerant endo-xylanase (GH11) from Humicola brevis var. thermoidea for lignocellulosic valorization of sugarcane biomass. Biocatal Agric Biotechnol 44:102424. https://doi.org/10.1016/j.bcab.2022.102424
Alokika A, Kumar A, Kumar V, Singh B (2021) Cellulosic and hemicellulosic fractions of sugarcane bagasse: potential, challenges and future perspective. Int J Biol Macromol 169:564–582. https://doi.org/10.1016/j.ijbiomac.2020.12.175
Alvira P, Negro MJ, Ballesteros M (2011) Effect of endoxylanase and α-L-arabinofuranosidase supplementation on the enzymatic hydrolysis of steam exploded wheat straw. Bioresour Technol 102(6):4552–4558. https://doi.org/10.1016/j.biortech.2010.12.112
Amo GS, Bezerra-Bussoli C, da Silva RR, Kishi LT, Ferreira H, Mariutti RB, Arni RK, Gomes E, Bonilla-Rodriguez GO (2019) Heterologous expression, purification and biochemical characterization of a new xylanase from Myceliophthora heterothallica. Inter J Biol Macromol 131:798–805. https://doi.org/10.1016/j.ijbiomac.2019.03.108
Anwar Z, Gulfraz M, Irshad M (2014) Agro-industrial lignocellulosic biomass a key to unlock the future bio-energy: a brief review. J Radiat Res Appl 7(2):163–173. https://doi.org/10.1016/j.jrras.2014.02.003
Ariffin H, Sapuan, SM, Hassan, MA (2019) Lignocellulose for future bioeconomy. In: Lignocellulose for future bioeconomy. https://doi.org/10.1016/C2018-0-00037-7
Ávila PF, Martins M, Costa FAA, Goldbeck R, Fibre D (2020) Xylooligosaccharides production by commercial enzyme mixture from agricultural wastes and their prebiotic and antioxidant potential. Bioact Carbohyd Diet Fibre 24:100234. https://doi.org/10.1016/j.bcdf.2020.100234
Bajaj P, Mahajan R (2019) Cellulase and xylanase synergism in industrial biotechnology. Appl Microbiol Biotechnol 103:8711–8724. https://doi.org/10.1007/s00253-019-10146-0
Banerjee J, Singh R, Vijayaraghavan R, MacFarlane D, Patti AF, Arora A (2017) Bioactives from fruit processing wastes: green approaches to valuable chemicals. Food Chem 225(1):10–22. https://doi.org/10.1016/j.foodchem.2016.12.093
Basit A, Liu J, Rahim K, Jiang W, Lou H (2018) Thermophilic xylanases: from bench to bottle. Crit Rev Biotechnol 38(7):989–1002. https://doi.org/10.1080/07388551.2018.1425662
Basotra N, Joshi S, Satyanarayana T, Pati PK, Tsang A, Chadha BS (2018) Expression of catalytically efficient xylanases from thermophilic fungus Malbranchea cinnamomea for synergistically enhancing hydrolysis of lignocellulosics. Inter J Biol Macromol 108:185–192. https://doi.org/10.1016/j.ijbiomac.2017.11.131
Bergmeyer, HU, Bernt E (1974) Lactate dehydro-genase. UV-assay with pyruvate and NADH. In: Bergmeyer HU (ed) Methods of enzymatic analysis. 2nd edn, Academic Press, New York, pp. 574–578
Bezerra TL, Ragauskas AJ (2016) A review of sugarcane bagasse for second-generation bioethanol and biopower production. Biofuels Bioprod Bioref 10(5):634–647. https://doi.org/10.1002/bbb.1662
Bhardwaj N, Agrawal K, Verma, P (2021) Xylanases: an overview of its Diverse Function in the Field of Biorefinery. In: Srivastava M, Srivastava N, Singh R. (eds) Bioenergy Research: Commercial Opportunities & Challenges. Clean Energy Production Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-16-1190-2_10
Bhattacharya AS, Bhattacharya A, Pletschke BI (2015) Synergism of fungal and bacterial cellulases and hemicellulases: a novel perspective for enhanced bio-ethanol production. Biotechnol Lett 37:1117–1129. https://doi.org/10.1007/s10529-015-1779-3
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254. https://doi.org/10.1006/abio.1976.9999
Brar KK, Santo MCE, Pellegrini VO, Azevedo ER, Guimarães FEC, Polikarpov I, Chadha BS (2020) Enhanced hydrolysis of hydrothermally and autohydrolytically treated sugarcane bagasse and understanding the structural changes leading to improved saccharification. Biomass Bioenerg 139:105639. https://doi.org/10.1016/j.biombioe.2020.105639
Bussamra BC, Freitas S, Costa ACJ (2015) Improvement on sugar cane bagasse hydrolysis using enzymatic mixture designed cocktail. Bioresour Technol 187:173–181. https://doi.org/10.1016/j.biortech.2015.03.117
Carvalho DM, Martínez-Abad A, Evtuguin DV, Colodette JL, Lindström ME, Vilaplana F, Sevastyanova O (2017) Isolation and characterization of acetylated glucuronoarabinoxylan from sugarcane bagasse and straw. Carb Polym 156:223–234. https://doi.org/10.1016/j.carbpol.2016.09.022
Carvalho AFA, Figueiredo FC, Campioni TS, Pastore GM, Neto PO (2020) Improvement of some chemical and biological methods for the efficient production of xylanases, xylooligosaccharides and lignocellulose from sugar cane bagasse. Biomass Bioenerg 143:105851. https://doi.org/10.1016/j.biombioe.2020.105851
Castro-Ochoa LD, Hernández-Leyva SR, Medina-Godoy S, Gómez-Rodríguez J, Aguilar-Uscanga MG, Martínez CC (2023) Integration of agricultural residues as biomass source to saccharification bioprocess and for the production of cellulases from filamentous fungi. Biotech 13:43. https://doi.org/10.1007/s13205-022-03444-4
Chadha BS, Ajay BK, Mellon F, Bhat MK (2004) Two endoxylanases active and stable at alkaline pH from the newly isolated thermophilic fungus, Myceliophthora sp. IMI 387099. J Biotechnol 109(3):227–237. https://doi.org/10.1016/j.jbiotec.2003.12.010
Chadha BS, Kaur B, Basotra N, Tsang A, Pandey A (2019) Thermostable xylanases from thermophilic fungi and bacteria: current perspective. Bioresour Technol 277:195–203. https://doi.org/10.1016/j.biortech.2019.01.044
Chanwicha N, Katekaew S, Aimi T, Boonlue S (2015) Purification and characterization of alkaline xylanase from Thermoascus aurantiacus var. levisporus KKU-PN-I2–1 cultivated by solid-state fermentation. Mycoscience. https://doi.org/10.1016/j.myc.2014.09.003
Cintra LC, Costa IC, Oliveira ICM, Fernandes AG, Faria SP, Jesuíno RSA, Ravanal MC, Eyzaguirre J, Ramos LP, Faria FP, Ulhoa CJ (2020) The boosting effect of recombinant hemicellulases on the enzymatic hydrolysis of steam-treated sugarcane bagasse. Enzyme Microb Technol 133:109447. https://doi.org/10.1016/j.enzmictec.2019.109447
Collard F-X, Blin J (2014) A review on pyrolysis of biomass constituents: mechanisms and composition of the products obtained from the conversion of cellulose, hemicelluloses and lignin. Renew Sust Energ Ver 38:594–608. https://doi.org/10.1016/j.rser.2014.06.013
Cota J, Oliveira LC, Damásio ARL, Citadini AP, Hoffmam ZB, Alvarez TM, Codima CA, Leite VBP, Pastore G, Oliveira-Neto M, Murakami MT, Ruller R (2013) Assembling a xylanase–lichenase chimera through all-atom molecular dynamics simulations. Biochim Biophys Acta 1834(8):1492–1500. https://doi.org/10.1016/j.bbapap.2013.02.030
Dar FM, Dar PM (2021) Fungal xylanases for different industrial applications. In: Abdel-Azeem AM, Yadav AN, Yadav N, Sharma M (eds) Industrially important fungi for sustainable development. Fungal biology. Springer, Cham. https://doi.org/10.1007/978-3-030-85603-8_14
Davis BJ (1964) Method and application to human serum protein. Ann NY Acad Sci 121:404–427. https://doi.org/10.1111/j.1749-6632.1964.tb14213.x
Du Y, Shi P, Huang H, Zhang X, Luo H, Wang Y, Yao B (2013) Characterization of three novel thermophilic xylanases from Humicola insolens Y1 with application potentials in the brewing industry. Bioresour Technol 130:161–167. https://doi.org/10.1016/j.biortech.2012.12.067
Duarte GC, Moreira LRS, Jaramillo PMD, Filho EXF (2012) Biomass-derived inhibitors of holocellulases. Bioenerg Res 5:768–777. https://doi.org/10.1007/s12155-012-9182-6
Espejo F (2021) Role of commercial enzymes in wine production: A critical review of recent research. J Food Sci Technol 58(1):9–21. https://doi.org/10.1007/s13197-020-04489-0
Filiatrault-Chastel C, Heiss-Blanquet S, Margeot A, Berrin J-GJBA (2021) From fungal secretomes to enzymes cocktails: the path forward to bioeconomy. Biotechnol Adv 52:10783. https://doi.org/10.1016/j.biotechadv.2021.107833
Gonçalves T, Damásio A, Segato F, Alvarez T, Bragatto J, Brenelli L, Citadini A, Murakami MT, Ruller R, Paes-Leme AP, Prade R, Squina FM (2012) Functional characterization and synergic action of fungal xylanase and arabinofuranosidase for production of xylooligosaccharides. Bioresour Technol 119:293–299. https://doi.org/10.1016/j.biortech.2012.05.062
Haldar D, Purkait MK (2020) Lignocellulosic conversion into value-added products: a review. Process Biochem 89:110–133. https://doi.org/10.1016/j.procbio.2019.10.001
Hinz SWA, Pouvreau LAM, Joosten R, Bartels J, Jonathan MC, Wery J, Schols HA (2009) Hemicellulase production in Chrysosporium lucknowense C1. J Cereal Sci 50:318–323. https://doi.org/10.1016/j.jcs.2009.07.005
Hu J, Arantes V, Saddler JN (2011) The enhancement of enzymatic hydrolysis of lignocellulosic substrates by the addition of accessory enzymes such as xylanase: is it an additive or synergistic effect? Biotechnol Biofuels 4:36. https://doi.org/10.1186/1754-6834-4-36
Hüttner S, Granchi Z, Nguyen TT, van Pelt S, Larsbrink J, Thanh VN, Olsson L (2018) Genome sequence of Rhizomucor pusillus FCH 5.7, a thermophilic zygomycete involved in plant biomass degradation harbouring putative GH9 endoglucanases. Biotechnol Reports 20:e00279. https://doi.org/10.1016/j.btre.2018.e00279
Jain KK, Bhanja Dey T, Kumar S, Kuhad RC (2015) Production of thermostable hydrolases (cellulases and xylanase) from Thermoascus aurantiacus RCKK: a potential fungus. Bioprocess Biosyst Eng 38(4):787–796. https://doi.org/10.1007/s00449-014-1320-4
Jugwanth Y, Sewsynker-Sukai Y, Kana E (2020) Valorization of sugarcane bagasse for bioethanol production through simultaneous saccharification and fermentation: optimization and kinetic studies. Fuel 262:116552. https://doi.org/10.1016/j.fuel.2019.116552
Julio R, Albet J, Vialle C, Vaca-Garcia C, Sablayrolles C (2017) Sustainable design of biorefinery processes: existing practices and new methodology. Biofuels Bioprod Biorefin 11(2):373–395. https://doi.org/10.1002/bbb.1749
Karnaouri A, Topakas E, Antonopoulou I, Christakopoulos P (2014) Genomic insights into the fungal lignocellulolytic system of Myceliophthora thermophila. Front Microbiol 5:281. https://doi.org/10.3389/fmicb.2014.00281
Kaushal J, Arya SK, Khatri M, Singh G, Nur NIW, Rajagopal R, Soon SW, Ravindran B, Awasthi MK (2022) Efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase (Xy) and MOF immobilized xylanase (Xy-Cu-BTC). Bioresour Technol 357:127374. https://doi.org/10.1016/j.biortech.2022.127374
Knob A, Carmona EC (2010) Purification and characterization of two extracellular xylanases from Penicillium sclerotiorum: a novel acidophilic xylanase. Appl Biochem Biotechnol 162(2):429–443. https://doi.org/10.1007/s12010-009-8731-8
Kostylev M, Wilson DJB (2012) Synergistic interactions in cellulose hydrolysis. Biofuels 3(1):61–70. https://doi.org/10.4155/bfs.11.150
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259):680–685. https://doi.org/10.1038/227680a0
Li X, Dilokpimol A, Kabel MA, de Vries RP (2022) Fungal xylanolytic enzymes: Diversity and applications. Bioresour Technol 344:126290. https://doi.org/10.1016/j.biortech.2021.126290
Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B (2014) The carbohydrate-active enzymes database (CAZy) in 2013. Nucleic Acids Res 42(D1):D490–D495. https://doi.org/10.1093/nar/gkt1178
Lopes AM, Ferreira Filho EX, Moreira LRS (2018) An update on enzymatic cocktails for lignocellulose breakdown. J Appl Microbiol 125(3):632–645. https://doi.org/10.1111/jam.13923
Maheshwari R, Bharadwaj G, Bhat MK (2000) Thermophilic fungi: their physiology and enzymes. Microbiol Mol Biol Rev 64(3):461–488. https://doi.org/10.1128/mmbr.64.3.461-488.2000
Manning M, Colón W (2004) Structural basis of protein kinetic stability: resistance to sodium dodecyl sulfate suggests a central role for rigidity and a bias toward β-sheet structure. Biochem 43(35):11248–11254. https://doi.org/10.1021/bi0491898
Mathibe BN, Malgas S, Radosavljevic L, Kumar V, Shukla P, Pletschke BI (2020) Lignocellulosic pretreatment-mediated phenolic by-products generation and their effect on the inhibition of an endo-1,4-β-xylanase from Thermomyces lanuginosus VAPS-24. 3Biotech 10(8):349. https://doi.org/10.1007/s13205-020-02343-w
McIlvaine TC (1921) A buffer solution for colorimetric comparison. J Biol Chem 49(1):183–186. https://doi.org/10.1016/S0021-9258(18)86000-8
McPhillips K, Waters DM, Parlet C, Walsh DJ, Arendt EK, Murray PG (2014) Purification and Characterization of a β-1,4-Xylanase from Remersonia thermophila CBS 540.69 and Its Application in Bread Making. Appl Biochem Biotechnol 172:1747–1762. https://doi.org/10.1007/s12010-013-0640-1
Michelin M, Ximenes E, de Moraes MLT, Ladisch MR (2016) Effect of phenolic compounds from pretreated sugarcane bagasse on cellulolytic and hemicellulolytic activities. Bioresour Technol 199:275–278. https://doi.org/10.1016/j.biortech.2015.08.120
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31(3):426–428. https://doi.org/10.1021/ac60147a030
Monclaro AV, Recalde GL, da Silva Jr FG, de Freitas SM, Ferreira Filho EX (2019) Xylanase from Aspergillus tamarii shows different kinetic parameters and substrate specificity in the presence of ferulic acid. Enzyme Microb Technol 120:16–22. https://doi.org/10.1016/j.enzmictec.2018.09.009
Monica P, Kapoor M (2021) Alkali-stable GH11 endo-β-1,4 xylanase (XynB) from Bacillus subtilis strain CAM 21: application in hydrolysis of agro-industrial wastes, fruit/vegetable peels and weeds. Prep Biochem Biotechnol 51(5):475–487. https://doi.org/10.1080/10826068.2020.1830416
Nakasu PYS, Chagas MF, Costa AC, Rabelo SC (2017) Kinetic study of the acid post-hydrolysis of xylooligosaccharides from hydrothermal pretreatment. Bioenerg Res 10:1045–1056. https://doi.org/10.1007/s12155-017-9864-1
Nascimento CEO, de Oliveira Simões LC, de Cassia PJ, da Silva RR, de Lima EA, de Almeida GC, Penna ALB, Boscolo M, Gomes E, da Silva R (2022) Application of a recombinant GH10 endoxylanase from Thermoascus aurantiacus for xylooligosaccharide production from sugarcane bagasse and probiotic bacterial growth. J Biotechnol 347:1–8. https://doi.org/10.1016/j.jbiotec.2022.02.003
Olopoda IA, Lawal OT, Omotoyinbo OV, Kolawole AN, Sanni DM (2022) Biochemical characterization of a thermally stable, acidophilic and surfactant-tolerant xylanase from Aspergillus awamori AFE1 and hydrolytic efficiency of its immobilized form. Process Biochem 121:45–55. https://doi.org/10.1016/j.procbio.2022.06.030
Pandey C, Sharma P, Gupta N (2023) Engineering to enhance thermostability of xylanase: for the new era of biotechnology. J Appl Biol Biotechnol 11(2):41–54. https://doi.org/10.7324/JABB.2023.110204
Pinales-Márquez CD, Rodríguez-Jasso RM, Araújo RG, Loredo-Treviño A, Nabarlatz D, Gullón B, Ruiz HA (2021) Circular bioeconomy and integrated biorefinery in the production of xylooligosaccharides from lignocellulosic biomass: a review. Ind Crops Prod 162:113274. https://doi.org/10.1016/j.indcrop.2021.113274
Poletto P, Pereira GN, Monteiro CR, Pereira MAF, Bordignon SE, de Oliveira D (2020) Xylooligosaccharides: transforming the lignocellulosic biomasses into valuable 5-carbon sugar prebiotics. Process Biochem 91:352–363. https://doi.org/10.1016/j.procbio.2020.01.005
Polizeli MLTM, Rizzatti A, Monti R, Terenzi HF, Jorge JA, Amorim DS (2005) Xylanases from fungi: properties and industrial applications. Appl Microbiol Biotechnol 67:577–591. https://doi.org/10.1007/s00253-005-1904-7
Pollet A, Delcour JA, Courtin CM (2010) Structural determinants of the substrate specificities of xylanases from different glycoside hydrolase families. Crit Rev Biotechnol 30(3):176–191. https://doi.org/10.3109/07388551003645599
Rashid R, Sohail M (2021) Xylanolytic Bacillus species for xylooligosaccharides production: a critical review. Bioresour Bioprocess 8(1):1–14. https://doi.org/10.1186/s40643-021-00369-3
Ribeiro LF, De Lucas RC, Vitcosque GL, Ribeiro LF, Ward RJ, Rubio MV, Damásio AR, Squina FM, Gregory RC, Walton PH, Jorge JA, Prade RA, Buckeridge MS, Polizeli MLTM (2014) A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology. Biotechnol Biofuels 7:115. https://doi.org/10.1186/1754-6834-7-115
Rodrigues PO, Moreira FS, Cardoso VC, Santos LD, Gurgel LVA, Pasquini D, Baffi MA (2022) Combination of high solid load, on-site enzyme cocktails and surfactant in the hydrolysis of hydrothermally pretreated sugarcane bagasse and ethanol production. Waste Biomass Valor 13:3085–3094. https://doi.org/10.1007/s12649-022-01685-1
Saha BC (2003) Hemicellulose bioconversion. J Ind Microbiol Biotechnol 30(5):279–291. https://doi.org/10.1007/s10295-003-0049-x
Saini JK, Kaur A, Mathur A (2022) Strategies to enhance enzymatic hydrolysis of lignocellulosic biomass for biorefinery applications: A review. Bioresour Technol 360:127517. https://doi.org/10.1016/j.biortech.2022.127517
Schmatz AA, Tyhoda L, Brienzo M (2020) Sugarcane biomass conversion influenced by lignin. Biofuels Bioprod Bioref 14(2):469–480. https://doi.org/10.1002/bbb.2070
Seemakram W, Boonrung S, Aimi T, Ekprasert J, Lumyong S, Boonlue S (2020) Purification, characterization and partial amino acid sequences of thermo-alkali-stable and mercury ion-tolerant xylanase from Thermomyces dupontii KKU–CLD–E2–3. Sci Rep 10(1):1–10. https://doi.org/10.1038/s41598-020-78670-y
Sharma M, Kumar A (2013) Xylanases: an overview. Br Biotechnol J 3(1):1–28
Sharma M, Chadha BS, Saini HS (2010) Purification and characterization of two thermostable xylanases from Malbranchea flava active under alkaline conditions. Bioresour Technol 101(22):8834–8842. https://doi.org/10.1016/j.biortech.2010.06.071
Sharma A, Sharma A, Singh S, Kuhad RC, Nain L (2019) Thermophilic fungi and their enzymes for biorefineries. In: Tiquia-Arashiro S, Grube M (eds) Fungi in extreme environments: ecological role and biotechnological significance. Springer Cham. https://doi.org/10.1007/978-3-030-19030-9_24
Silva LAO, Terrasan CRF, Carmona EC (2015a) Purification and characterization of xylanases from Trichoderma inhamatum. Electron J Biotechnol 18(4):307–313. https://doi.org/10.1016/j.ejbt.2015.06.00
Silva COG, Aquino EN, Ricart CAO, Midorikawa GEO, Filho EXF (2015b) GH11 xylanase from Emericella nidulans with low sensitivity to inhibition by ethanol and lignocellulose-derived phenolic compounds. FEMS Microbiol Lett 362(13):fnv094. https://doi.org/10.1093/femsle/fnv094
Silva PO, Guimarães NCA, Serpa JDM, Masui DC, Marchetti CR, Verbisck NV, Zanoelo FF, Ruller R, Giannesi GC (2019) Application of an endo-xylanase from Aspergillus japonicus in the fruit juice clarification and fruit peel waste hydrolysis. Biocatal Agric Biotechnol 21:101312. https://doi.org/10.1016/j.bcab.2019.101312
Sindhu R, Binod P, Pandey A (2016) Biological pretreatment of lignocellulosic biomass—an overview. Bioresour Technol 199:76–82. https://doi.org/10.1016/j.biortech.2015.08.030
Singh G, Kaur S, Khatri M, Arya SK (2019) Biobleaching for pulp and paper industry in India: Emerging enzyme technology. Biocat Agric Biotechnol 17:558–565. https://doi.org/10.1016/j.bcab.2019.01.019
Teixeira RSS, Siqueira FG, Souza MVD, Filho EXF, Bon EPS (2010) Purification and characterization studies of a thermostable β-xylanase from Aspergillus awamori. J Ind Microbiol Biotechnol 37(10):1041–1051. https://doi.org/10.1007/s10295-010-0751-4
Tuohy M, Puls J, Claeyssens M, Vršanská M, Coughlan MP (1993) The xylan-degrading enzyme system of Talaromyces emersonii: novel enzymes with activity against aryl β-D-xylosides and unsubstituted xylans. Biochem J 290(2):515–523. https://doi.org/10.1042/bj2900515
Ustinov BB, Gusakov AV, Antonov AI, Sinitsyn AP (2008) Comparison of properties and mode of action of six secreted xylanases from Chrysosporium lucknowense. Enzyme Microb Technol 43(1):56–65. https://doi.org/10.1016/j.enzmictec.2008.01.017
Vafiadi C, Christakopoulos P, Topakas E (2010) Purification, characterization and mass spectrometric identification of two thermophilic xylanases from Sporotrichum thermophile. Process Biochem 45(3):419–424. https://doi.org/10.1016/j.procbio.2009.10.009
Van den Brink J, van Muiswinkel GC, Theelen B, Hinz SW, de Vries RP (2013) Efficient plant biomass degradation by thermophilic fungus Myceliophthora heterothallica. Appl Environ Microbiol 79(4):1316–1324. https://doi.org/10.1128/AEM.02865-12
Vries JG (2023) Industrial implementation of chemical biomass conversion. Curr Opin Green Sustain Chem 39:100715. https://doi.org/10.1016/j.cogsc.2022.100715
Winger A, Heazlewood J, Chan L, Petzold C, Permaul K, Singh S (2014) Secretome analysis of the thermophilic xylanase hyper-producer Thermomyces lanuginosus SSBP cultivated on corn cobs. J Ind Microbiol Biotechnol 41(11):1687–1696. https://doi.org/10.1007/s10295-014-1509-1
Yousuf A, o Pirozzi D, Sannino F (eds) (2019) Fundamentals of lignocellulosic biomass. In: Lignocellulosic biomass to liquid biofuels. 1st edn, Academic Press, Massachusetts, pp. 1–15. https://doi.org/10.1016/C2017-0-03529-2
Zanoelo FF, Polizeli MLTM, Terenzi HF, Jorge JA (2004) Purification and biochemical properties of a thermostable xylose-tolerant β-D-xylosidase from Scytalidium thermophilum. J Ind Microbiol Biotechnol 31(4):170–176. https://doi.org/10.1007/s10295-004-0129-6
Zanoni JA, de Oliveira IB, Perrone OM, Ortega JO, Boscolo M, Gomes E, Bonilla-Rodriguez GO (2021) Production and biochemical characterization of xylanases synthesized by the thermophilic fungus Rasamsonia emersonii S10 by solid-state cultivation. Ecletica Quim 46(1SI):53–67. https://doi.org/10.26850/1678-4618eqj.v46.1SI
Zhao Z, Xian M, Liu M, Zhao G (2020) Biochemical routes for uptake and conversion of xylose by microorganisms. Biotechnol Biofuels 13:1–12. https://doi.org/10.1186/s13068-020-1662-x
Zimbardi AL, Sehn C, Meleiro LP, Souza FH, Masui DC, Nozawa MS, Guimarães LH, Jorge JA, Furriel RPM (2013) Optimization of β-glucosidase, β-xylosidase and xylanase production by Colletotrichum graminicola under solid-state fermentation and application in raw sugarcane trash saccharification. Int J Mol Sci 14(2):2875–2902. https://doi.org/10.3390/ijms14022875
Acknowledgements
This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001, in addition to providing scholarship to the first author. This work was supported by the Universidade Federal de Mato Grosso do Sul–UFMS/MEC Brazil. We are grateful for the Multicenter Graduate Program in Biochemistry and Molecular Biology (PMBqBM- SBBq) Brazil.
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Franco, D.G., de Almeida, A.P., Galeano, R.M.S. et al. Exploring the potential of a new thermotolerant xylanase from Rasamsonia composticola (XylRc): production using agro-residues, biochemical studies, and application to sugarcane bagasse saccharification. 3 Biotech 14, 3 (2024). https://doi.org/10.1007/s13205-023-03844-0
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s13205-023-03844-0