Skip to main content
SpringerLink
Log in

Screening of IR50 × Rathu Heenati F7 RILs and Identification of SSR Markers Linked to Brown Planthopper (Nilaparvata lugens Stål) Resistance in Rice (Oryza sativa L.)

  • Research
  • Published:
Molecular Biotechnology Aims and scope Submit manuscript

Abstract

Brown planthopper (Nilaparvata lugens Stål) is one of the major insect pests of rice. A Sri Lankan indica rice cultivar Rathu Heenati was found to be resistant to all biotypes of the brown planthopper. In the present study, a total of 268 F7 RILs of IR50 and Rathu Heenati were phenotyped for their level of resistance against BPH by the standard seedbox screening test (SSST) in the greenhouse. A total of 53 SSR primers mapped on the chromosome 3 were used to screen the polymorphism between the parents IR50 and Rathu Heenati, out of which eleven were found to be polymorphic between IR50 and Rathu Heenati. The eleven primers that have shown polymorphism between the IR50 and Rathu Heenati parents were genotyped in a set of five resistant RILs and five susceptible RILs along with the parents for co-segregation analysis. Among the eleven primers, two primers namely RM3180 (18.22 Mb) and RM2453 (20.19 Mb) showed complete co-segregation with resistance. The identification of SSR markers linked with BPH resistant could be used for the maker assisted selection (MAS) program in rice breeding and to map the resistant genes on rice chromosomes for further gene cloning.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Alam, S. N., & Cohen, M. B. (1998). Detection and analysis of QTLs for resistance to the brown planthopper, Nilaparvata lugens, in a doubled-haploid rice population. Theoretical and Applied Genetics, 97, 1370–1379.

    Article  CAS  Google Scholar 

  2. Ausubel, F. M., Brent, R., Kinston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., et al. (1994). Current protocols in molecular biology. New York: Wiley.

    Google Scholar 

  3. Bonman, J. M., Khush, G. S., & Nelson, R. J. (1992). Breeding rice for resistance to pests. Annual Review of Phytopathology, 30, 507–528.

    Article  Google Scholar 

  4. Buna, W., Zhen, H., Lihui, S., Xiang, R., Xiang, L., Xianghua, L., et al. (2001). Mapping of two new brown planthopper resistance genes from wild rice. Chinese Science Bulletin, 46, 1092–1095.

    Article  Google Scholar 

  5. Chen, J. W., Wang, L., Pang, X. F., & Pan, Q. H. (2006). Genetic analysis and fine mapping of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph19(t). Molecular Genetics and Genomics, 275, 321–329.

    Article  CAS  Google Scholar 

  6. Gomathi, S. K. (2002). Identification of genetic locus associated with resistance to brown planthopper (Nilaparvata lugens Stål) in rice (Oryza sativa L.). PhD Thesis, Tamil Nadu Agricultural University, Coimbatore.

  7. Heinrichs, E. A. (1979). Control of leafhopper and planthopper vectors of rice viruses. In K. Moramorosch & K. F. Arris (Eds.), Leafhopper vectors and planthopper disease agents (pp. 529–558). New York: Academic Press.

    Google Scholar 

  8. Heinrichs, E. A., Medrano, F. G., & Rapusas, H. R. (1985). Genetic evaluation for insect resistance in rice (p. 365). Manila, Philippines: International Rice Research Institute.

    Google Scholar 

  9. Hirabayashi, H., Kaji, R., Angeles, E. R., Ogawa, T., Brar, D. S., & Khush, G. S. (1999). RFLP analysis of a new gene for resistance to brown planthopper derived from O. officinalis on rice chromosome 4. Breeding Science, 49 (Suppl. 1), 48. (abstract in Japanese).

    Google Scholar 

  10. Hirabayashi, H., Kaji R., Okamoto M., Ogawa, T., Brar, D. S., Angeles, E. R., & Khush, G. S. (1998). Mapping QTLs for brown planthopper (BPH) resistance introgressed from Oryza officinalis in rice. Advances in rice genetics, 268–270.

  11. Huang, Z., He, G., Shu, L., Li, X., & Zhang, Q. (2001). Identification and mapping of two brown planthopper resistance genes in rice. Theoretical and Applied Genetics, 102, 929–934.

    Article  CAS  Google Scholar 

  12. Ikeda, R. (1985). Studies on the inheritance of resistance to the rice brown planthopper (Nilaparvata lugens Stål) and the breeding of resistant rice cultivars. Bulletin of National Agricultural Research Centre, 3, 1–54. (in Japanese).

    Google Scholar 

  13. Ikeda, R., & Kaneda, C. (1981). Genetic analysis of resistance to brown planthopper, Nilaparvata lugens Stål, in rice. Japanese Journal of Breeding, 31, 279–285.

    Google Scholar 

  14. IRRI. (1988). Standard evaluation systems for rice. International Rice Research Institute, Manila, Philippines.

  15. Ishii, T., Brar, D. S., Multani, D. S., & Khush, G. S. (1994). Molecular tagging of genes for brown planthopper resistance and earliness introgressed from Oryza australiensis into cultivated rice, O. sativa. Genome, 37, 217–221.

    Article  CAS  Google Scholar 

  16. Jairin, J., Phengrat, K., Teangdeerith, S., Vanavichit, A., & Toojinda, T. (2007). Mapping of broad spectrum brown planthopper resistance gene, Bph3, on rice chromosome 6. Molecular Breeding, 19, 35–44.

    Article  CAS  Google Scholar 

  17. Jairin, J., Teangdeerith, S., Leelagud, P., Phengrat, K., Vanavichit, A., & Toojinda, T. (2007). Physical mapping of Bph3, a brown planthopper resistance locus in rice. Maejo International Journal of Science and Technology, 01, 166–177.

    CAS  Google Scholar 

  18. Jena, K. K., Jeung, J. U., Lee, J. H., Choi, H. C., & Brar, D. S. (2006). High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph 18(t). and marker assisted selection for BPH resistance in rice (Oryza sativa L.). Theoretical and Applied Genetics, 112, 288–297.

    Article  CAS  Google Scholar 

  19. Jena, K. K., & Khush, G. S. (1990). Introgression of genes from Oryza officinalis Well ex. Watt to cultivated rice, O. sativa L. Theoretical and Applied Genetics, 80, 737–745.

    Article  Google Scholar 

  20. Jena, K. K., Pasalu, I. C., Rao, Y. K., Varalaxmi, Y., Krishnaiah, K., Khush, G. S., et al. (2002). Molecular tagging of a gene for resistance to brown planthopper in rice (Oryza sativa L.). Eyphytica, 129, 81–88.

    Article  Google Scholar 

  21. Jennifer, M. S. (2006). Identification of simple sequence repeat loci associated with resistance to brown planthopper (Nilaparvata lugens stål) in F 4 families of IR50/Rathu Heenati of rice (Oryza sativa L.). PhD Thesis, Tamil Nadu Agricultural University, Coimbatore.

  22. Kabir, M. A., & Khush, G. S. (1988). Genetic analysis of resistance of brown planthoppers in rice, Oryza sativa L. Plant Breeding, 100, 54–58.

    Article  Google Scholar 

  23. Kawaguchi, M., Murata, K., Ishii, T., Takumi, S., Mori, N., & Nakamura, C. (2001). Assignment of a brown planthopper (Nilaparvata lugens Stål.) resistance gene bph4 to the rice chromosome 6. Breeding Science, 51, 13–18.

    Article  CAS  Google Scholar 

  24. Khush, G.S. (1979).Genetics of and breeding resistance to the brown planthopper. In Brown planthopper : Threat to rice production in Asia (pp. 321–332). Los Banos, Philippines: IRRI.

  25. Khush, G. S., & Brar, D. S. (1991). Genetics of resistance to insects in crop plants. Advances in Agronomy, 45, 223–274.

    Article  Google Scholar 

  26. Khush, G. S., Karim, A. N. M. R., & Angeles, E. R. (1985). Genetics of resistance of rice cultivar ARC 10550 to Bangladesh brown planthopper biotype. Journal of Genetics, 64, 121–125.

    Article  Google Scholar 

  27. Lakshminarayana, A., & Khush, G. S. (1977). New genes for resistance to brown planthopper in rice. Crop Science, 17, 96–100.

    Article  Google Scholar 

  28. Martinez, C. R., & Khush, G. S. (1974). Sources and inheritance of resistance to brown planthopper in some breeding lines of rice (Oryza sativa L.). Crop Science, 14, 264–267.

    Article  Google Scholar 

  29. Myint, K. K. M., Matsumura, M., Takagi, M., & Yasui, H. (2009). Demographic parameters of long-term laboratory strains of the brown planthopper, Nilaparvata lugens stål, (Homoptera: Delphacidae) on resistance genes, bph20(t) and Bph21(t) in rice. Journal of the Faculty of Agriculture Kyushu University, 54(1), 159–164.

    CAS  Google Scholar 

  30. Nemoto, H., Ikeda, R., & Kaneda, C. (1989). New genes for resistance to brown planthopper, Nilaparvata lugens Stål, in rice. Japanese Journal of Breeding, 39, 23–28.

    CAS  Google Scholar 

  31. Panda, N., & Khush, G. S. (1995). Host plant resistance to insects. London, UK: CAB International.

    Google Scholar 

  32. Rahman, M. L., Jiang, W., Chu, S. H., Qiao, Y., Ham, T. H., Woo, M. K., et al. (2009). High-resolution mapping of two rice brown planthopper resistance genes, Bph20(t) and Bph21(t), originating from Oryza minuta. Theoretical and Applied Genetics, 119, 1237–1246.

    Article  Google Scholar 

  33. Ramalingam, J., Cruz, C. M. V., Kukereja, K., Chittor, J. M., Wu, J. L., Lee, S. W., et al. (2003). Candidate defence genes from rice, barley, and maize and their association with qualitative resistance in rice. Molecular Plant-Microbe Interactions, 16, 14–24.

    Article  CAS  Google Scholar 

  34. Ren, X., Wang, X., Yuan, X., Weng, Q., Zhu, L., & He, G. (2004). Mapping quantitative trait loci and expressed sequence tags related to brown planthopper resistance in rice. Plant Breeding, 123, 342–348.

    Article  CAS  Google Scholar 

  35. Renganayaki, K., Friz, A. K., Sadasivam, S., Pammi, S., Harrington, S. E., McCouch, S. R., et al. (2002). Mapping and progress toward map-based cloning of brown planthopper biotype-4 resistance gene introgressed from Oryza officinalis into cultivated rice, O. sativa. Crop Science, 42, 2112–2117.

    Article  CAS  Google Scholar 

  36. Sharma, P. N., Ketipearachchi, Y., Murata, K., Torii, A., Takumi, S., Mori, N., et al. (2003). RFLP/AFLP mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph1 in rice. Euphytica, 129, 109–117.

    Article  CAS  Google Scholar 

  37. Soundararajan, R. P., Kadirvel, P., Gunathilagaraj, K., & Maheswaran, M. (2004). Mapping of quantitative trait loci associated with resistance to brown planthopper in rice by means of a doubled haploid population. Crop Science, 44, 2214–2220.

    Article  CAS  Google Scholar 

  38. Sun, L., Liu, Y., Jiang, L., Su, C., Wang, C., Zhai, H., et al. (2007). Identification of quantitative trait loci associated with resistance to brown planthopper in the indica rice cultivar Col.5 Thailand. Hereditas, 144, 48–52.

    Article  Google Scholar 

  39. Sun, L., Su, C., Wang, C., Zhai, H., & Wan, J. (2005). Mapping of a major resistance gene to the brown planthopper in the rice cultivar Rathu Heenati. Breeding Science, 55, 391–396.

    Article  CAS  Google Scholar 

  40. Tan, G. X., Weng, Q. M., Ren, X., Huang, Z., Zhu, L., & He, G. C. (2004). Two whitebacked planthopper resistance genes in rice share the same loci with those for brown planthopper resistance. Heredity, 9, 212–217.

    Article  CAS  Google Scholar 

  41. Xu, X. F., Mei, H. W., Luo, L. J., Cheng, X. N., & Li, Z. K. (2002). RFLP-facilitated investigation of the quantitative resistance of rice to brown planthopper (Nilaparvata lugens stål). Theoretical and Applied Genetics, 104, 248–253.

    Article  CAS  Google Scholar 

  42. Xu, Y., Zhu, L., Xiao, J., Huang, N., & McCouch, S. R. (1997). Chromosomal regions associated with segregation distortion of molecular markers in F2, backcross. Double haploid and recombinant inbred populations in rice (Oryza sativa L.). Theoretical and Applied Genetics, 253, 535–545.

    CAS  Google Scholar 

  43. Yang, H. Y., You, A. Q., Yang, Z. F., Zhang, F., He, R. F., Zhu, L. L., et al. (2004). High resolution genetic mapping at the Bph15 locus for brown planthopper resistance in rice (Oryza sativa L.). Theoretical and Applied Genetics, 110, 182–191.

    Article  CAS  Google Scholar 

  44. Yarasi, B., Sadumpati, V., Immanni, C. P., Vudem, D. R., & Khareedu, V. R. (2008). Transgenic rice expressing Allium sativum leaf agglutinin (ASAL) exhibits high-level resistance against major sap-sucking pests. BMC Plant Biology., 8, 102–115.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The financial support of Department of Biotechnology, Government of India through Government of India-Center for Plant Molecular Biology scheme to Tamil Nadu Agricultural University is greatly acknowledged.

Author information

Authors and Affiliations

  1. Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore, 641 003, India

    Sanju Kumari, Jennifer M. Sheba, Nagarajan Pothi, Mohankumar Subbarayalu, Raveendran Muthurajan & Senthil Natesan

  2. Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, 641 003, India

    Maheshwaran Marappan & Shanmugasunderam Ponnuswamy

  3. Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, 641 003, India

    Suresh Seetharaman

Authors
  1. Sanju Kumari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jennifer M. Sheba
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Maheshwaran Marappan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shanmugasunderam Ponnuswamy
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Suresh Seetharaman
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Nagarajan Pothi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mohankumar Subbarayalu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Raveendran Muthurajan
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Senthil Natesan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Senthil Natesan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kumari, S., M. Sheba, J., Marappan, M. et al. Screening of IR50 × Rathu Heenati F7 RILs and Identification of SSR Markers Linked to Brown Planthopper (Nilaparvata lugens Stål) Resistance in Rice (Oryza sativa L.). Mol Biotechnol 46, 63–71 (2010). https://doi.org/10.1007/s12033-010-9279-0

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12033-010-9279-0

Keywords

Search

Navigation