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Molecular mapping and transfer of a novel brown planthopper resistance gene bph42 from Oryza rufipogon (Griff.) To cultivated rice (Oryza sativa L.)

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Abstract

Background

Brown planthopper (BPH), Nilaparvata lugens (Stål), is one of the most destructive pests of rice accounting for 52% of annual yield loss. The breakdown of resistance against known BPH biotypes necessitates the identification and deployment of new genes from diverse sources. The current study aimed at mapping and transfer of a novel BPH resistance gene from the wild species of rice O. rufipogon accession CR100441 to the elite rice cultivar against BPH biotype 4.

Methods and Results

The phenotypic screening against BPH biotype 4 was conducted using the standard seedbox screening technique (SSST). Inheritance study using damage score caused by BPH infestation at the seedling stage indicated the presence of a single major recessive gene with the segregation ratio of susceptible to resistant plants in 3:1 (210:66, χ2c = 0.17 ≤ χ20.05,1 = 3.84). The genotyping of the mapping population was done using polymorphic microsatellite markers between PR122 and O.rufipogon acc.CR100441 spanning all the 12 chromosomes of rice. A total of 537 SSR markers were used to map a BPH resistance gene (designated as bph42) on the short arm of chromosome 4 between RM16282 and RM6659. QTL analysis identified a peak marker RM16335 contributing 29% of the phenotypic variance at 40.76 LOD.

Conclusions

The identified marker co-segregates with the bph42 and hence could be efficiently used for marker-assisted selection (MAS) for the transfer of resistance into elite rice cultivars. The introgression lines with higher yield and BPH resistance were identified and are under advanced yield trails for further varietal release.

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Data Availability

All the data generated and analyzed in this study are available in this article as supplementary material.

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Acknowledgements

The authors gratefully acknowledge the National Rice Research Institute, Cuttack for providing seed material. This work was supported by ICAR under the Project ‘Molecular breeding for improvement of tolerance to biotic and abiotic stresses, yield and quality traits in Crops-Rice, Department of Biotechnology, New Delhi (BT/PR31; 75/ATGC/127/5/2019) and Department of Science and Technology, New Delhi (CRG/2018/001833).

Funding

This work was supported by ICAR under the Project ‘Molecular breeding for improvement of tolerance to biotic and abiotic stresses, yield and quality traits in Crops-Rice, Department of Biotechnology, New Delhi (BT/PR31; 75/ATGC/127/5/2019) and Department of Science and Technology, New Delhi (CRG/2018/001833).

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Authors and Affiliations

  1. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, India

    Pavneet Kaur, Kumari Neelam, Ankita Babbar, Kishor Kumar, Yogesh Vikal & Kuldeep Singh

  2. Department of Genetics and Plant Breeding, Punjab Agricultural University, Ludhiana, Punjab, India

    Preetinder Singh Sarao, Renu Khanna, Rupinder Kaur & Gurjeet Singh Mangat

  3. Integrated Rural Development and Management Faculty Centre, Ramakrishna Mission Vivekananda Educational and Research Institute, 700103, Narendrapur, Kolkata, India

    Kishor Kumar

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  1. Pavneet Kaur
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  2. Kumari Neelam
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Contributions

Kumari Neelam, Kuldeep Singh, Yogesh Vikal: Conceptualization of the research, proofreading of the manuscript; Pavneet Kaur carried out all the genotyping and phenotyping of the mapping population; Kumari Neelam, Kishor Kumar, Ankita Babbar: Investigation, data analysis, writing of the original draft; Preetinder Singh Sarao provided BPH biotype 4 and screening facility; Renu Khanna, Rupinder Kaur, Gurjeet Singh Mangat: Handling of segregating generation, breeding and generation advancement, management of multi-location trials. All co-authors approved this manuscript before submission.”

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Correspondence to Kumari Neelam.

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Kaur, P., Neelam, K., Sarao, P.S. et al. Molecular mapping and transfer of a novel brown planthopper resistance gene bph42 from Oryza rufipogon (Griff.) To cultivated rice (Oryza sativa L.). Mol Biol Rep 49, 8597–8606 (2022). https://doi.org/10.1007/s11033-022-07692-8

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