Advertisement

Chinese Science Bulletin

, Volume 58, Issue 18, pp 2168–2173 | Cite as

FISH of 5S rDNA and telomeric (TTAGGG) n repeats in normal and translocated populations of the frog Quasipaa boulengeri (Anura, Ranidae)

  • LiYan Qing
  • Yun Xia
  • YuChi Zheng
  • XiaoMao Zeng
Open Access
Article Special Issue Adaptive Evolution and Conservation Ecology of Wild Animals
First Online:

Abstract

Quasipaa boulengeri, a spiny frog, is widely distributed in the low mountain regions, around Sichuan Basin. Our previous study revealed five karyotypes, caused by a translocation, that are randomly distributed throughout different populations. 5S rDNA and telomere sequence (TTAGGG)n are potential good markers for chromosome identification and karyological evolution. In this study, we examined the sequences of 14 populations using fluorescence in situ hybridization (FISH) to detect if there is any variation between karyologically normal and translocated populations. 5S rDNA loci were located at the same position on chromosomes 1 in 7 translocated populations. In two of the seven normal populations, 5S rDNA also occurred on chromosome 5 in addition to chromosome 1. Our findings further indicate that the 5S rDNA on No. 1 most likely represents the ancestral condition, while the minor loci represent the derived state. Signal density variations of the 5S rDNA were observed beteween homologous chromosomes or sister chromatids of pair 1 in both normal and translocated populations. Telomere sequences were identically located on all ends of the 26 chromosomes in seven rearranged populations, however, no ITSs were observed on the translocated chromosomes 1 and 6. Two of the six normal populations were found to contain ITSs which indicates that populations with translocation events diverged prior to those with ITSs rearrangements. In the KKS and BF populations, the ITSs of chromosome 3 are not always found on both homologues. Inter-chromosomal signal strength of telomeric sequences commonly differs within all populations.

Keywords

Quasipaa boulengeri fluorescence in situ hybridization (FISH) 5S rDNA telomere (TTAGGG)n sequence intraspecific 
Download to read the full article text

References

  1. 1.
    Vitelli L, Batistoni R, Andronico F, et al. Chromosomal localization of 18S + 28S and 5S ribosomal RNA genes in evolutionarily diverse anuran amphibians. Chromosoma, 1982, 84: 475–491CrossRefGoogle Scholar
  2. 2.
    Ferreira I A, Oliveira C, Venere P C, et al. 5S rDNA variation and its phylogenetic inference in the genus Leporinus (Characiformes: Anostomidae). Genetica, 2007, 129: 253–257CrossRefGoogle Scholar
  3. 3.
    Úbeda-Manzanaro M, Merlo M A, Palazón J L, et al. Chromosomal mapping of the major and minor ribosomal genes, (GATA)n and U2 snRNA gene by double-colour FISH in species of the Batrachoididae family. Genetica, 2010, 138: 787–794CrossRefGoogle Scholar
  4. 4.
    Martins C, Galetti P. Chromosomal localization of 5S rDNA genes in Leporinus fish (Anostomidae, Characiformes). Chromosome Res, 1999, 7: 363–367CrossRefGoogle Scholar
  5. 5.
    Schmid M, Steinlein C, Bogart J P, et al. The chromosomes of terraranan frogs. Insights into vertebrate cytogenetics. Cytogenet Genome Res, 2010, 130-131: 1–568CrossRefGoogle Scholar
  6. 6.
    Nascimento J, Quinderé Y R S D, Recco-Pimentel S M, et al. Heteromorphic Z and W sex chromosomes in Physalaemus ephippifer (Steindachner, 1864)(Anura, Leiuperidae). Genetica, 2010, 138: 1127–1132CrossRefGoogle Scholar
  7. 7.
    León P E, Kezer J. Localization of 5S RNA genes on chromosomes of plethodontid salamanders. Chromosoma, 1978, 65: 213–230CrossRefGoogle Scholar
  8. 8.
    Lucchini S D, Nardi I, Barsacchi G, et al. Molecular cytogenetics of the ribosomal (18S + 28S and 5S) DNA loci in primitive and advanced urodele amphibians. Genome, 1993, 36: 762–773CrossRefGoogle Scholar
  9. 9.
    Nardi I, Lucchini S, Batistoni R, et al. Cytogenetics of the European plethodontid salamanders of the genus Hydromantes (Amphibia, Urodela). Chromosoma, 1986, 94: 377–388CrossRefGoogle Scholar
  10. 10.
    Nardi I. Cytogenetics of the European plethodontid salamanders, genus Hydromantes. In: Green D M, Sessions S K, eds. Amphibian Cytogenetics and Evolution. San Diego: Academic Press, 1991. 131–156Google Scholar
  11. 11.
    Schmid M, Feichtinger W, Steinlein C, et al. Chromosome banding in Amphibia XXVI. Coexistence of homomorphic XY sex chromosomes and a derived Y-autosome translocation in Eleutherodactylus maussi (Anura, Leptodactylidae). Cytogenet Genome Res, 2002, 99: 330–343CrossRefGoogle Scholar
  12. 12.
    Barsacchi-Pilone G, Nardi I, Andronico F, et al. Chromosomal location of the ribosomal RNA genes in Triturus vulgaris meridionalis (Amphibia, Urodela). Chromosoma, 1977, 63: 127–134CrossRefGoogle Scholar
  13. 13.
    Meyne J, Baker R J, Hobart H H, et al. Distribution of non-telomeric sites of the (TTAGGG)n telomeric sequence in vertebrate chromosomes. Chromosoma, 1990, 99: 3–10CrossRefGoogle Scholar
  14. 14.
    Bolzán A D, Bianchi M S. Telomeres, interstitial telomeric repeat sequences, and chromosomal aberrations. Mutat Res/Rev Mutat, 2006, 612: 189–214CrossRefGoogle Scholar
  15. 15.
    Amaro-Ghilardi R, Silva M, Rodrigues M, et al. Chromosomal studies in four species of genus Chaunus (Bufonidae, Anura): Localization of telomeric and ribosomal sequences after fluorescence in situ hybridization (FISH). Genetica, 2008, 134: 159–168CrossRefGoogle Scholar
  16. 16.
    Amaro-Ghilardi R C, Rodrigues M T, Yonenaga-Yassuda Y. Chromosomal studies after differential staining and fluorescence in situ hybridization using telomeric probe in three Leptodactylus species (Leptodactylidae, Anura). Caryologia, 2004, 57: 53–65CrossRefGoogle Scholar
  17. 17.
    Wiley J, Meyne J, Little M, et al. Interstitial hybridization sites of the (TTAGGG)n telomeric sequence on the chromosomes of some North American hylid frogs. Cytogenet Genome Res, 1992, 61: 55–57CrossRefGoogle Scholar
  18. 18.
    Wiley J, Little M. Replication banding patterns of the diploid-tetraploid treefrogs Hyla chrysoscelis and H. versicolor. Cytogenet Genome Res, 2000, 88: 11–14CrossRefGoogle Scholar
  19. 19.
    Carvalho K A, Garcia P C A, Recco-Pimentel S M. NOR dispersion, telomeric sequence detection in centromeric regions and meiotic multivalent configurations in species of the Aplastodiscus albofrenatus group (Anura, Hylidae). Cytogenet Genome Res, 2009, 126: 359–367CrossRefGoogle Scholar
  20. 20.
    Stock M, Steinlein C, Lamatsch D K, et al. Multiple origins of tetraploid taxa in the Eurasian Bufo viridis subgroup. Genetica, 2005, 124: 255–272CrossRefGoogle Scholar
  21. 21.
    Schmid M, Feichtinger W, Steinlein C, et al. Chromosome banding in Amphibia — XXIII. Giant W sex chromosomes and extremely small genomes in Eleutherodactylus euphronides and Eleutherodactylus shrevei (Anura, Leptodactylidae). Cytogenet Genome Res, 2002, 97: 81–94CrossRefGoogle Scholar
  22. 22.
    Foote D, Wiley J, Little M, et al. Ribosomal RNA gene site polymorphism in Bufo terrestris. Cytogenet Genome Res, 1991, 57: 196–199CrossRefGoogle Scholar
  23. 23.
    Wang D. Chromosome research on Paa bouelngeri and Paa yunnanensis (Ranidae: Paa) (in Chinese). Master Dissertation. Chengdu: Sichuan University, 2006Google Scholar
  24. 24.
    Qing L, Xia Y, Zheng Y, et al. A de novo case of floating chromosomal polymorphism by translocation in Qaasipaa boulengeri (Anura. Dicloglossidae). PLoS One, 2012, 7: e46163CrossRefGoogle Scholar
  25. 25.
    Sambrook J, Russell D. Molecular Cloning: A Laboratory Manual. 3rd ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 2001Google Scholar
  26. 26.
    Bi K, Bogart J P, Fu J. A populational survey of 45S rDNA polymorphism in the Jefferson salamander Ambystoma jeffersonianum revealed by fluorescence in situ hybridization (FISH). Curr Zool, 2009, 55: 145–149Google Scholar
  27. 27.
    Sola L, De Innocentiis S, Gornung E, et al. Cytogenetic analysis of Epinephelus marginatus (Pisces: Serranidae), with the chromosome localization of the 18S and 5S rRNA genes and of the (TTAGGG)n telomeric sequence. Mar Biol, 2000, 137: 47–51CrossRefGoogle Scholar
  28. 28.
    Zhang L L, Bao Z M, Wang S, et al. Chromosome rearrangements in Pectinidae (Bivalvia: Pteriomorphia) implied based on chromosomal localization of histone H3 gene in four scallops. Genetica, 2007, 130: 193–198CrossRefGoogle Scholar
  29. 29.
    Hutchison N, Pardue M. The mitotic chromosomes of Notophthalmus (=Triturus) viridescens: Localization of C banding regions and DNA sequences complementary to 18S, 28S and 5S ribosomal RNA. Chromosoma, 1975, 53: 51–69CrossRefGoogle Scholar
  30. 30.
    Moran P, Martinez J L, Garcia-Vazquez E, et al. Sex chromosome linkage of 5S rDNA in rainbow trout (Oncorhynchus mykiss). Cytogenet Cell Genet, 1996, 75: 145–150CrossRefGoogle Scholar
  31. 31.
    Boroń A, Ozouf-Costaz C, Coutanceau J P, et al. Gene mapping of 28S and 5S rDNA sites in the spined loach Cobitis taenia (Pisces, Cobitidae) from a diploid population and a diploid-tetraploid population. Genetica, 2006, 128: 71–79CrossRefGoogle Scholar
  32. 32.
    Schmid M, Vitelli L, Batistoni R. Chromosome banding in amphibia. XI. Constitutive heterochromatin, nucleolus organizers, 18S + 28S and 5S ribosomal RNA genes in Ascaphidae, Pipidae, Discoglossidae and Pelobatidae. Chromosoma, 1987, 95: 271–284CrossRefGoogle Scholar
  33. 33.
    King M, Contreras N, Honeycutt R L. Variation within and between nucleolar organizer regions in Australian hylid frogs (Anura) shown by 18S+ 28S in-situ hybridization. Genetica, 1990, 80: 17–29CrossRefGoogle Scholar
  34. 34.
    Schmid M. Chromosome banding in Amphibia. VII. Analysis of the structure and variability of NORs in Anura. Chromosoma, 1982, 87: 327–344CrossRefGoogle Scholar
  35. 35.
    Fagundes V, Vianna-Morgante A, Yonenaga-Yassuda Y. Telomeric sequences localization and G-banding patterns in the identification of a polymorphic chromosomal rearrangement in the rodent Akodon cursor (2n=14, 15 and 16). Chromosome Res, 1997, 5: 228–232CrossRefGoogle Scholar
  36. 36.
    Nanda I, Schrama D, Feichtinger W, et al. Distribution of telomeric (TTAGGG)n sequences in avian chromosomes. Chromosoma, 2002, 111: 215–227CrossRefGoogle Scholar
  37. 37.
    Baker R, Sakai R, Saifuddin U, et al. Translocations in the mosquito, Culex tritaeniorhynchus. J Hered, 1977, 68: 157–166Google Scholar
  38. 38.
    Nanda I, Fugate M, Steinlein C, et al. Distribution of (TTAGGG)n telomeric sequences in karyotypes of the Xenopus species complex. Cytogenet Genome Res, 2008, 122: 396–400CrossRefGoogle Scholar

Copyright information

© The Author(s) 2013

Authors and Affiliations

  • LiYan Qing
    • 1
    • 2
  • Yun Xia
    • 1
    • 2
  • YuChi Zheng
    • 1
  • XiaoMao Zeng
    • 1
  1. 1.Chengdu Institute of BiologyChinese Academy of SciencesChengduChina
  2. 2.University of Chinese Academy of SciencesBeijingChina

Personalised recommendations