Skip to main content

FISH of 5S rDNA and telomeric (TTAGGG) n repeats in normal and translocated populations of the frog Quasipaa boulengeri (Anura, Ranidae)

Abstract

Quasipaa boulengeri, a spiny frog, is widely distributed in the low mountain regions, around Sichuan Basin. Our previous study revealed five karyotypes, caused by a translocation, that are randomly distributed throughout different populations. 5S rDNA and telomere sequence (TTAGGG)n are potential good markers for chromosome identification and karyological evolution. In this study, we examined the sequences of 14 populations using fluorescence in situ hybridization (FISH) to detect if there is any variation between karyologically normal and translocated populations. 5S rDNA loci were located at the same position on chromosomes 1 in 7 translocated populations. In two of the seven normal populations, 5S rDNA also occurred on chromosome 5 in addition to chromosome 1. Our findings further indicate that the 5S rDNA on No. 1 most likely represents the ancestral condition, while the minor loci represent the derived state. Signal density variations of the 5S rDNA were observed beteween homologous chromosomes or sister chromatids of pair 1 in both normal and translocated populations. Telomere sequences were identically located on all ends of the 26 chromosomes in seven rearranged populations, however, no ITSs were observed on the translocated chromosomes 1 and 6. Two of the six normal populations were found to contain ITSs which indicates that populations with translocation events diverged prior to those with ITSs rearrangements. In the KKS and BF populations, the ITSs of chromosome 3 are not always found on both homologues. Inter-chromosomal signal strength of telomeric sequences commonly differs within all populations.

References

  1. Vitelli L, Batistoni R, Andronico F, et al. Chromosomal localization of 18S + 28S and 5S ribosomal RNA genes in evolutionarily diverse anuran amphibians. Chromosoma, 1982, 84: 475–491

    Article  Google Scholar 

  2. Ferreira I A, Oliveira C, Venere P C, et al. 5S rDNA variation and its phylogenetic inference in the genus Leporinus (Characiformes: Anostomidae). Genetica, 2007, 129: 253–257

    Article  Google Scholar 

  3. Úbeda-Manzanaro M, Merlo M A, Palazón J L, et al. Chromosomal mapping of the major and minor ribosomal genes, (GATA)n and U2 snRNA gene by double-colour FISH in species of the Batrachoididae family. Genetica, 2010, 138: 787–794

    Article  Google Scholar 

  4. Martins C, Galetti P. Chromosomal localization of 5S rDNA genes in Leporinus fish (Anostomidae, Characiformes). Chromosome Res, 1999, 7: 363–367

    Article  Google Scholar 

  5. Schmid M, Steinlein C, Bogart J P, et al. The chromosomes of terraranan frogs. Insights into vertebrate cytogenetics. Cytogenet Genome Res, 2010, 130-131: 1–568

    Article  Google Scholar 

  6. Nascimento J, Quinderé Y R S D, Recco-Pimentel S M, et al. Heteromorphic Z and W sex chromosomes in Physalaemus ephippifer (Steindachner, 1864)(Anura, Leiuperidae). Genetica, 2010, 138: 1127–1132

    Article  Google Scholar 

  7. León P E, Kezer J. Localization of 5S RNA genes on chromosomes of plethodontid salamanders. Chromosoma, 1978, 65: 213–230

    Article  Google Scholar 

  8. Lucchini S D, Nardi I, Barsacchi G, et al. Molecular cytogenetics of the ribosomal (18S + 28S and 5S) DNA loci in primitive and advanced urodele amphibians. Genome, 1993, 36: 762–773

    Article  Google Scholar 

  9. Nardi I, Lucchini S, Batistoni R, et al. Cytogenetics of the European plethodontid salamanders of the genus Hydromantes (Amphibia, Urodela). Chromosoma, 1986, 94: 377–388

    Article  Google Scholar 

  10. Nardi I. Cytogenetics of the European plethodontid salamanders, genus Hydromantes. In: Green D M, Sessions S K, eds. Amphibian Cytogenetics and Evolution. San Diego: Academic Press, 1991. 131–156

    Google Scholar 

  11. Schmid M, Feichtinger W, Steinlein C, et al. Chromosome banding in Amphibia XXVI. Coexistence of homomorphic XY sex chromosomes and a derived Y-autosome translocation in Eleutherodactylus maussi (Anura, Leptodactylidae). Cytogenet Genome Res, 2002, 99: 330–343

    Article  Google Scholar 

  12. Barsacchi-Pilone G, Nardi I, Andronico F, et al. Chromosomal location of the ribosomal RNA genes in Triturus vulgaris meridionalis (Amphibia, Urodela). Chromosoma, 1977, 63: 127–134

    Article  Google Scholar 

  13. Meyne J, Baker R J, Hobart H H, et al. Distribution of non-telomeric sites of the (TTAGGG)n telomeric sequence in vertebrate chromosomes. Chromosoma, 1990, 99: 3–10

    Article  Google Scholar 

  14. Bolzán A D, Bianchi M S. Telomeres, interstitial telomeric repeat sequences, and chromosomal aberrations. Mutat Res/Rev Mutat, 2006, 612: 189–214

    Article  Google Scholar 

  15. Amaro-Ghilardi R, Silva M, Rodrigues M, et al. Chromosomal studies in four species of genus Chaunus (Bufonidae, Anura): Localization of telomeric and ribosomal sequences after fluorescence in situ hybridization (FISH). Genetica, 2008, 134: 159–168

    Article  Google Scholar 

  16. Amaro-Ghilardi R C, Rodrigues M T, Yonenaga-Yassuda Y. Chromosomal studies after differential staining and fluorescence in situ hybridization using telomeric probe in three Leptodactylus species (Leptodactylidae, Anura). Caryologia, 2004, 57: 53–65

    Article  Google Scholar 

  17. Wiley J, Meyne J, Little M, et al. Interstitial hybridization sites of the (TTAGGG)n telomeric sequence on the chromosomes of some North American hylid frogs. Cytogenet Genome Res, 1992, 61: 55–57

    Article  Google Scholar 

  18. Wiley J, Little M. Replication banding patterns of the diploid-tetraploid treefrogs Hyla chrysoscelis and H. versicolor. Cytogenet Genome Res, 2000, 88: 11–14

    Article  Google Scholar 

  19. Carvalho K A, Garcia P C A, Recco-Pimentel S M. NOR dispersion, telomeric sequence detection in centromeric regions and meiotic multivalent configurations in species of the Aplastodiscus albofrenatus group (Anura, Hylidae). Cytogenet Genome Res, 2009, 126: 359–367

    Article  Google Scholar 

  20. Stock M, Steinlein C, Lamatsch D K, et al. Multiple origins of tetraploid taxa in the Eurasian Bufo viridis subgroup. Genetica, 2005, 124: 255–272

    Article  Google Scholar 

  21. Schmid M, Feichtinger W, Steinlein C, et al. Chromosome banding in Amphibia — XXIII. Giant W sex chromosomes and extremely small genomes in Eleutherodactylus euphronides and Eleutherodactylus shrevei (Anura, Leptodactylidae). Cytogenet Genome Res, 2002, 97: 81–94

    Article  Google Scholar 

  22. Foote D, Wiley J, Little M, et al. Ribosomal RNA gene site polymorphism in Bufo terrestris. Cytogenet Genome Res, 1991, 57: 196–199

    Article  Google Scholar 

  23. Wang D. Chromosome research on Paa bouelngeri and Paa yunnanensis (Ranidae: Paa) (in Chinese). Master Dissertation. Chengdu: Sichuan University, 2006

    Google Scholar 

  24. Qing L, Xia Y, Zheng Y, et al. A de novo case of floating chromosomal polymorphism by translocation in Qaasipaa boulengeri (Anura. Dicloglossidae). PLoS One, 2012, 7: e46163

    Article  Google Scholar 

  25. Sambrook J, Russell D. Molecular Cloning: A Laboratory Manual. 3rd ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 2001

    Google Scholar 

  26. Bi K, Bogart J P, Fu J. A populational survey of 45S rDNA polymorphism in the Jefferson salamander Ambystoma jeffersonianum revealed by fluorescence in situ hybridization (FISH). Curr Zool, 2009, 55: 145–149

    Google Scholar 

  27. Sola L, De Innocentiis S, Gornung E, et al. Cytogenetic analysis of Epinephelus marginatus (Pisces: Serranidae), with the chromosome localization of the 18S and 5S rRNA genes and of the (TTAGGG)n telomeric sequence. Mar Biol, 2000, 137: 47–51

    Article  Google Scholar 

  28. Zhang L L, Bao Z M, Wang S, et al. Chromosome rearrangements in Pectinidae (Bivalvia: Pteriomorphia) implied based on chromosomal localization of histone H3 gene in four scallops. Genetica, 2007, 130: 193–198

    Article  Google Scholar 

  29. Hutchison N, Pardue M. The mitotic chromosomes of Notophthalmus (=Triturus) viridescens: Localization of C banding regions and DNA sequences complementary to 18S, 28S and 5S ribosomal RNA. Chromosoma, 1975, 53: 51–69

    Article  Google Scholar 

  30. Moran P, Martinez J L, Garcia-Vazquez E, et al. Sex chromosome linkage of 5S rDNA in rainbow trout (Oncorhynchus mykiss). Cytogenet Cell Genet, 1996, 75: 145–150

    Article  Google Scholar 

  31. Boroń A, Ozouf-Costaz C, Coutanceau J P, et al. Gene mapping of 28S and 5S rDNA sites in the spined loach Cobitis taenia (Pisces, Cobitidae) from a diploid population and a diploid-tetraploid population. Genetica, 2006, 128: 71–79

    Article  Google Scholar 

  32. Schmid M, Vitelli L, Batistoni R. Chromosome banding in amphibia. XI. Constitutive heterochromatin, nucleolus organizers, 18S + 28S and 5S ribosomal RNA genes in Ascaphidae, Pipidae, Discoglossidae and Pelobatidae. Chromosoma, 1987, 95: 271–284

    Article  Google Scholar 

  33. King M, Contreras N, Honeycutt R L. Variation within and between nucleolar organizer regions in Australian hylid frogs (Anura) shown by 18S+ 28S in-situ hybridization. Genetica, 1990, 80: 17–29

    Article  Google Scholar 

  34. Schmid M. Chromosome banding in Amphibia. VII. Analysis of the structure and variability of NORs in Anura. Chromosoma, 1982, 87: 327–344

    Article  Google Scholar 

  35. Fagundes V, Vianna-Morgante A, Yonenaga-Yassuda Y. Telomeric sequences localization and G-banding patterns in the identification of a polymorphic chromosomal rearrangement in the rodent Akodon cursor (2n=14, 15 and 16). Chromosome Res, 1997, 5: 228–232

    Article  Google Scholar 

  36. Nanda I, Schrama D, Feichtinger W, et al. Distribution of telomeric (TTAGGG)n sequences in avian chromosomes. Chromosoma, 2002, 111: 215–227

    Article  Google Scholar 

  37. Baker R, Sakai R, Saifuddin U, et al. Translocations in the mosquito, Culex tritaeniorhynchus. J Hered, 1977, 68: 157–166

    Google Scholar 

  38. Nanda I, Fugate M, Steinlein C, et al. Distribution of (TTAGGG)n telomeric sequences in karyotypes of the Xenopus species complex. Cytogenet Genome Res, 2008, 122: 396–400

    Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to XiaoMao Zeng.

Additional information

This article is published with open access at Springerlink.com

Rights and permissions

This article is published under an open access license. Please check the 'Copyright Information' section either on this page or in the PDF for details of this license and what re-use is permitted. If your intended use exceeds what is permitted by the license or if you are unable to locate the licence and re-use information, please contact the Rights and Permissions team.

About this article

Cite this article

Qing, L., Xia, Y., Zheng, Y. et al. FISH of 5S rDNA and telomeric (TTAGGG) n repeats in normal and translocated populations of the frog Quasipaa boulengeri (Anura, Ranidae). Chin. Sci. Bull. 58, 2168–2173 (2013). https://doi.org/10.1007/s11434-013-5690-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11434-013-5690-9

Keywords

  • Quasipaa boulengeri
  • fluorescence in situ hybridization (FISH)
  • 5S rDNA
  • telomere (TTAGGG)n sequence
  • intraspecific