Abstract
The metaphase chromosomes of Notophthalmus (Triturus) viridescens have been studied by C-banding and in situ hybridization. The chromosomes show the pericentric C-banding seen in many organisms and in addition have interstitial C-bands located a short distance from the pericentric C-bands on each chromosome arm. A few C-bands are seen in telomeric regions. Regions which hybridize in situ with 18S and 28S ribosomal RNA were found on three chromosome pairs. The animals studied fell into three groups with respect to which of the six possible sites showed detectable hybridization with 18S and 28S RNA. Individual animals differed not only in the pattern of in situ hybridization of ribosomal RNA but also in the number of ribosomal RNA cistrons in the genome as measured by saturation hybridization on purified DNA. In situ hybridization showed five pairs of chromosomes which contained DNA complementary to 5S RNA. The four pairs of subtelocentric chromosomes in the N. viridescens karyotype all have 5S DNA in the pericentric regions. The fifth cluster of 5S DNA is in the middle of one arm of the chromosomes in one of the two smallest submetacentric pairs in the genome. The five sites of 5S DNA differ markedly in the level of in situ hybridization with 5S cRNA.
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Barsacchi, G., Gall, J. G.: Chromosomal localization of repetitive DNA in the newt, Triturus. J. Cell Biol. 54, 580–591 (1972)
Brown, D. D., Wensink, P. C., Jordan E.: Purification and some characteristics of 5S DNA from Xenopus laevis. Proc. nat. Acad. Sci. (Wash.) 68, 3175–3179 (1971)
Callan, H. G.: Heterochromatin in Triton. Proc. roy. Soc., Lond., B 130, 324–335 (1942)
Callan, H. G.: Chromosomes and nucleoli of the axolotl, Ambystoma mexicanum. J. Cell. Sci. 1, 85–108 (1966)
Callan, H. G., Lloyd, L.: Working maps of the lampbrush chromosomes of amphibia. In: Handbook of genetics, IV (R. C. King, ed.). New York: Plenum Press 1975
Evans, H. J., Buckland, R. A., Pardue, M. L.: Location of the genes coding for 18S and 28S ribosomal RNA in the human genome. Chromosoma (Berl.) 48, 405–426 (1974)
Gall, J. G.: Lampbrush chromosomes from oocyte nuclei of the newt. J. Morph. 94, 283–352 (1954)
Gillespie, D., Spiegelman, S.: A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. molec. Biol. 12, 829–842 (1965)
Henderson, A. S., Warburton, D., Atwood, K. C.: Location of ribosomal DNA in the human chromosome complement. Proc. nat. Acad. Sci. (Wash.) 96, 3394–3398 (1972)
Hennen, S., Mizuno, S., Macgregor, H. C.: In situ hybridization of ribosomal DNA labeled with 125Iodine to metaphase and lampbrush chromosomes from newts. Chromosoma (Berl.) 50, 349–369 (1975)
Hsu, T. C., Spirito, S. E., Pardue, M. L.: Distribution of 18S and 28S ribosomal genes in mammalian genomes. Chromosoma (Berl.) 53, 25–36 (1975)
Labroussée, M., Guillemin, C., Gallien, M. L.: Mise en évidence sur les chromosomes de l'Amphibien Pleurodeles waltlii Michah. de secteurs d'affinité différente pour le colorant de Giemsa à pH 9. C. R. Acad. Sci. Paris, D. 274, 1063–1065 (1972)
Macgregor, H. C., Kezer, J.: The nucleolar organizer of Plethodon cinereus cinereus (Green). I. Location of the nucleolar organizer by in situ nucleic acid hybridization. Chromosoma (Berl.) 42, 415–426 (1973)
Mancino, G., Ragghianti, M., Innocenti, S. B.: I cariotipi di Triturus marmoratus e Triturus cristatus studiati con il “C-staining method”. Rend. Acc. Naz. Lincei (Roma) 55, 559–564 (1973)
Miller, L., Brown, D. D.: Variation in the activity of nucleolar organizers and their ribosomal gene content. Chromosoma (Berl.) 28, 430–444 (1969)
Nardi, I., Ragghianti, M., Mancino, G.: Banding patterns in newt chromosomes by the Giemsa stain. Chromosoma (Berl.) 40, 321–331 (1973)
Pardue, M. L.: Localization of repeated DNA sequences in Xenopus chromosomes. Cold Spr. Harb. Symp. quant. Biol. 38, 475–482 (1974)
Pardue, M. L., Brown, D. D., Birnstiel, M. L.: Localization of the genes for 5S ribosomal RNA in Xenopus laevis. Chromosoma (Berl.) 42, 191–203 (1973)
Pardue, M. L., Gall, J. G.: Molecular cytogenetics. In: Molecular genetics and developmental biology. Symp. Soc. Gen. Physiol., Woods Hole, Mass. 1971 (M. Sussman, ed.). Englewood Cliffs, New Jersey: Prentice-Hall 1972
Pardue, M. L., Gall, J. G.: Nucleic acid hybridization to the DNA of cytological preparations. In: Methods in cell biology, vol. 10. (D. M. Prescott, ed.). New York: Academic Press (in press, 1975)
Pardue, M. L., Hsu, T. C.: Locations of 18S and 28S ribosomal genes on the chromosomes of the Indian muntjac. J. Cell Biol. 64, 251–254 (1975)
Paris Conference (1971): Standardization in Human Cytogenetics. Birth Defects: Original Article Series, VIII: 7. New York: The National Foundation 1972
Pilone, G. B., Nardi, I., Batistoni, R., Andronico, F., Beccari, E.: Chromosome location of the genes for 28S, 18S and 5S ribosomal RNA in Triturus marmoratus (Amphibia Urodela). Chromosoma (Berl.) 49, 135–153 (1974)
Pukkila, P. J.: Identification of the lampbrush chromosome loops which transcribe 5S ribosomal RNA in Notophthalmus (Triturus) viridescens. Chromosoma (Berl.) 53, 71–89 (1975)
Reeder, R. H., Brown, D. D.: Transcription of the ribosomal RNA genes of an amphibian by the RNA polymerase of a bacterium. J. molec. Biol. 51, 171–183 (1970)
Ritossa, F. M., Atwood, K. C., Spiegelman, S.: A molecular explanation of the bobbed mutants of Drosophila as partial defiencies of “ribosomal” DNA. Genetics 54, 819–834 (1966)
Sinclair, J. H., Carroll, C. R., Humphrey, R. R.: Variation in rDNA redundancy level and nucleolar organizer length in normal and variant lines of the Mexican axolotl. J. Cell Sci. 15, 239–257 (1974)
Wallace, H., Birnstiel, M. L.: Ribosomal cistrons and the nucleolar organizer. Biochim. biophys. Acta (Amst.) 114, 296–310 (1966)
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Hutchison, N., Pardue, M.L. The mitotic chromosomes of Notophthalmus (=Triturus) viridescens: Localization of C banding regions and DNA sequences complementary to 18S, 28S and 5S ribosomal RNA. Chromosoma 53, 51–69 (1975). https://doi.org/10.1007/BF00329390
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DOI: https://doi.org/10.1007/BF00329390