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Chromosomal mapping of the major and minor ribosomal genes, (GATA)n and U2 snRNA gene by double-colour FISH in species of the Batrachoididae family

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Abstract

In the present study dual-colour fluorescence in situ hybridization (FISH) was performed to study the chromosomal distribution of 18S and 5S rDNAs, (GATA)n and 5S rDNA, and U2 snRNA and 18S rDNA in four species of Batrachoididae family: Amphichthys cryptocentrus, Batrachoides manglae, Porichthys plectrodon and Thalassophryne maculosa. The 18S rDNA signals were present in only one pair of chromosomes in all the four Batrachoididae species. The 5S rDNA was mapped on one pair of chromosomes, except in B. manglae, which showed a hybridization signal in two pairs. The two ribosomal genes are located on different chromosome pairs, except in A. cryptocentrus, in which they appear co-located. In all the cases, the (GATA)n probe produced disperse hybridization signals in all four species. The U2 snRNA signals appear very widely scattered in A. cryptocentrus, P. plectrodon, but show a degree of clustering in a specific chromosome pair in B. manglae. In T. maculosa, they are thinly dispersed and strong hybridization signals are observed co-located to the 18S rDNA-bearing chromosomes. Finally, a double-colour FISH with U2 snRNA and 5S rDNA probes was performed in B. manglae, and this showed that these genes were not co-located. These results have been compared with those from another Batrachoididae species, and evolutive processes of these species are discussed.

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Acknowledgments

María Úbeda-Manzanaro holds a CSIC JAE-Pre. fellowship. This work forms part of the Proyecto de Excelencia/IP: Carmen Sarasquete (RNM-03074; Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía, Spain).

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Authors and Affiliations

  1. Instituto de Ciencias Marinas de Andalucía, CSIC, Polígono Río San Pedro, 11510, Puerto Real, Cádiz, Spain

    María Úbeda-Manzanaro & Carmen Sarasquete

  2. Laboratorio Genética, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Polígono Río San Pedro, 11510, Puerto Real, Cádiz, Spain

    Manuel A. Merlo, Ismael Cross & Laureana Rebordinos

  3. Instituto de Investigaciones Científicas, Universidad de Oriente, Apdo. 147, Boca del Río, Isla Margarita, Venezuela

    José L. Palazón

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  1. María Úbeda-Manzanaro
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  4. Ismael Cross
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Correspondence to Laureana Rebordinos.

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Úbeda-Manzanaro, M., Merlo, M.A., Palazón, J.L. et al. Chromosomal mapping of the major and minor ribosomal genes, (GATA)n and U2 snRNA gene by double-colour FISH in species of the Batrachoididae family. Genetica 138, 787–794 (2010). https://doi.org/10.1007/s10709-010-9460-1

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  • DOI: https://doi.org/10.1007/s10709-010-9460-1

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