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Precise mapping of a quantitative trait locus interval for spike length and grain weight in bread wheat (Triticum aestivum L.)

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Abstract

The spike characteristics length, spikelet density and fertile floret number are related yield components and are important in cereal improvement. QSpl.nau-2D is a major quantitative trait locus controlling spike length (SPL) detected in the recombinant inbred line population developed by crossing wheat (Triticum aestivum) cultivars Nanda2419 with Wangshuibai. In this study, to validate its genetic effect and determine its precise location, QSpl.nau-2D’s near-isogenic line (NIL) was developed using Mianyang99-323 as the recurrent parent through marker-assisted selection. Field trials showed that the NIL not only had significantly longer spikes on average than the recurrent parent but also had significantly higher grain weight, but did not differ in spikelet number and kernel number per spike. In the F2 population derived from a cross of the NIL with Mianyang99-323, QSpl.nau-2D functioned like a single gene and conditioned the SPL in a partially dominant manner, and was thus designated as HL1 (for head length). To precisely map HL1, 89 recombinants, consisting of 11 genotypes, were identified in the NIL-derived F2 population of 674 plants by using markers in the Xwmc25–Xgpw4080 interval. Phenotyping these lines showed that the introduction of a 0.9-cM interval flanked by Xcfd53 and DG371 in Nanda2419 resulted in longer spikes and a higher grain weight in the NIL. The availability of markers closely linked to HL1 could facilitate its use in breeding programs.

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Acknowledgments

We are grateful to Mingcheng Luo at University of California, Davis, for providing SNP sequences. This study was partially supported by ‘973’ program (2011CB100103), ‘863’ program (2012AA101105), NSFC programs (30025030, 30721140555), Seed Funds for the Central Universities (2012), ‘111’ project B08025, and PAPD project of Jiangsu Higher Education Institutions.

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  1. The Applied Plant Genomics Laboratory of Crop Genomics and Bioinformatics Centre, National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agricultural Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Xinyi Wu, Ruiru Cheng, Shulin Xue, Zhongxin Kong, Hongshen Wan, Guoqiang Li, Yulong Huang, Haiyan Jia, Lixia Zhang & Zhengqiang Ma

  2. Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Jizeng Jia

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Wu, X., Cheng, R., Xue, S. et al. Precise mapping of a quantitative trait locus interval for spike length and grain weight in bread wheat (Triticum aestivum L.). Mol Breeding 33, 129–138 (2014). https://doi.org/10.1007/s11032-013-9939-4

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