Skip to main content
SpringerLink
Log in

Mapping of quantitative trait loci controlling partial resistance against rust incited by Uromyces pisi (Pers.) Wint. in a Pisum fulvum L. intraspecific cross

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

A quantitative trait loci (QTL) associated with resistance to pea rust, caused by the fungus Uromyces pisi (Pers.) Wint., has been identified in a F2 population derived from an intraspecific cross between two wild pea (Pisum fulvum L.) accessions, IFPI3260 (resistant) and IFPI3251 (susceptible). Both parental lines and all the segregating population displayed a fully compatible interaction (high infection type), which indicates absence of hypersensitive response. Nevertheless, differences on the percentage of symptomatic area of the whole plant (disease severity) were observed. A genetic map was developed covering 1283.3 cM and including 146 markers (144 random amplified polymorphic DNA (RAPDs) and two sequence tagged sites (STSs) markers) distributed in 9 linkage groups. A QTL explaining 63% of the total phenotypic variation was located in linkage group 3. RAPDs markers (OPY111316 and OPV171078) flanking this QTL should allow, after their conversion in SCARs, a reliable marker-assisted selection for rust resistance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Barilli E, Sillero JC, Fernández-Aparicio M, Rubiales D (2009a) Identification of resistance to Uromyces pisi (Pers.) Wint. in Pisum spp. germplasm. Field Crop Res 114:198–203

    Article  Google Scholar 

  • Barilli E, Sillero JC, Rubiales D (2009b) Characterization of resistance mechanisms to Uromyces pisi in pea. Plant Breed 128:665–670

    Article  Google Scholar 

  • Barilli E, Sillero JC, Serrano A, Rubiales D (2009c) Differential response of pea (Pisum sativum) to rusts incited by Uromyces viciae-fabae and U. pisi. Crop Prot 28:980–986

    Article  Google Scholar 

  • Chand R, Srivastava CP, Singh BD, Sarode SB (2006) Identification and characterization of slow rusting components in pea (Pisum sativum L.). Genet Resour Crop Evol 53:219–224

    Article  Google Scholar 

  • Choi HK, Luckow MA, Doyle J, Cook DR (2006) Development of nuclear gene-derived molecular markers linked to legume genetic maps. Mol Genet Genomics 276:56–70

    Article  CAS  PubMed  Google Scholar 

  • Cobos MJ, Fernández MJ, Rubio J, Kharrat M, Moreno MT, Gil J, Millán T (2005) A linkage map in chickpea (Cicer arietinum L.) in two populations from Kabuli × Desi crosses: location of a resistance gene for fusarium wilt race 0. Theor Appl Genet 110:1347–1353

    Article  CAS  PubMed  Google Scholar 

  • Ellwood SR, Phan HTT, Jordan M, Hanes J, Torres AM, Ávila CM, Cruz-Izquierdo S, Oliver RP (2008) Construction of a comparative genetic map in faba bean (Vicia faba L.) conservation of genome structure with Lens culinaris. BMC Genomics 9:380. doi:10.1186/1471-2164-9-380

    Article  PubMed  Google Scholar 

  • Emeran AA, Sillero JC, Niks RE, Rubiales D (2005) Morphology of infection structures help to distinguish among rust fungi infecting leguminous crops. Plant Dis 89:17–22

    Article  Google Scholar 

  • Fondevilla S, Carver TLW, Moreno MT, Rubiales D (2007) Identification and characterization of sources of resistance to Erysiphe pisi Syd. in Pisum spp. Plant Breed 126:113–119

    Article  Google Scholar 

  • Fondevilla S, Satovic Z, Rubiales D, Moreno MT, Torres AM (2008) Mapping of quantitative trait loci for resistance to Mycosphaerella pinodes in Pisum sativum subsp. syriacum. Mol Breed 21:439–454

    Article  CAS  Google Scholar 

  • Gilpin BJ, McCallum JA, Frew TJ, Timmerman-Vaughn GM (1997) A linkage map of the pea (Pisum sativum L.) genome containing cloned sequences of known function and expressed sequence tags (ESTs). Theor Appl Genet 114:59–66

    Google Scholar 

  • Gutiérrez MV, Vaz Patto MC, Huguet T, Cubero JI, Moreno MT, Torres AM (2005) Cross-species amplification of Medicago truncatula microsatellites across three major pulse crops. Theor Appl Genet 110:1210–1217

    Article  PubMed  Google Scholar 

  • Katiyar RP, Ram RS (1987) Genetics of rust resistance in pea. Indian J Genet 47:46–48

    Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugenics 12:461–465

    Google Scholar 

  • Kushwaha C, Chand R, Srivastava C (2006) Role of aeciospores in outbreaks of pea (Pisum sativum) rust (Uromyces fabae). Eur J Plant Pathol 115:323–330

    Article  Google Scholar 

  • Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199

    CAS  PubMed  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer program for constructing genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  CAS  PubMed  Google Scholar 

  • Lassner MW, Peterson P, Yoder JI (1989) Simultaneous amplification of multiple DNA fragments by polymerase chain reaction in the analysis of transgenic plants and their progeny. Plant Mol Biol Report 7:116–128

    Article  CAS  Google Scholar 

  • Li S (2009) Reaction of soybean rust-resistant lines identified in Paraguay to Mississippi isolates of Phakopsora pachyrhizi. Crop Sci 49:887–894

    Article  Google Scholar 

  • Loridon K, Mcphee K, Morin J, Dubreuil P, Pilet-Nayel ML, Aubert G, Rameau C, Baranger A, Coyne C, Lejeune-Hènaut I, Burstin J (2005) Microsatellite marker polymorphism and mapping in pea (Pisum sativum L.). Theor Appl Genet 111:1022–1031

    Article  CAS  PubMed  Google Scholar 

  • Lynch M, Walsh B (1997) Genetics and analysis of quantitative traits. Sinauer Associated Inc., Sunderland

    Google Scholar 

  • Madrid E, Rubiales D, Moral A, Moreno MT, Millan T, Gil J, Rubio J (2008) Mechanism and molecular markers associated with rust resistance in a chickpea interspecific cross (Cicer arietinum × Cicer reticulatum). Eur J Plant Pathol 121:43–53

    Article  CAS  Google Scholar 

  • Martínez F, Niks RE, Singh RP, Rubiales D (2001) Characterization of Lr46, a gene conferring partial resistance to wheat leaf rust. Hereditas 135:111–114

    Article  PubMed  Google Scholar 

  • Nelson M, Phan H, Ellwood S, Moolhuijzen P, Hane J, Williams A, O’Lone C, Fosu-Nyarko J, Scobie M, Cakir M, Jones M, Bellgard M, Ksiazkiewicz M, Wolko B, Barker S, Oliver R, Cowling W (2006) The first gene-based map of Lupinus angustifolius L.—location of domestication genes and conserved synteny with Medicago truncatula. Theor Appl Genet 113:225–238

    Article  CAS  PubMed  Google Scholar 

  • Niks RE, Rubiales D (2002) Potentially durable resistance mechanisms in plant to specialized fungal pathogens. Euphytica 124:201–216

    Article  CAS  Google Scholar 

  • Pal AB, Brahmappa HS, Rawal RD, Ullasa BA (1980) Field resistance of pea germplasm to powdery mildew (Erysiphe polygoni) and rust (Uromyces fabae). Plant Dis 64:1085–1086

    Google Scholar 

  • Pfunder M, Roy BA (2000) Pollinator-mediated interactions between a pathogenic fungus, Uromyces pisi (Pucciniaceae), and its host plant, Euphorbia cyparissias (Euphorbiaceae). Am J Bot 87:48–55

    Article  PubMed  Google Scholar 

  • Phan HTT, Ellwood SR, Hane JK, Ford R, Materne M, Oliver RP (2007) Extensive macrosynteny between Medicago truncatula and Lens culinaris ssp. culinaris. Theor Appl Genet 114:549–558

    Article  PubMed  Google Scholar 

  • Pilet PE (1952) Etude physiologique du parasitisme de l’Uromyces pisi (Pers.) de By., sur l’Euphorbia cyparissias L. Experientia 9:300–302

    Article  Google Scholar 

  • Prioul S, Frankevits A, Deniot G, Morin G, Baranger A (2004) Mapping of quantitative trait loci for partial resistance to Mycosphaerella pinodes in pea (Pisum sativum L.) at the seedling and adult stage. Theor Appl Genet 108:1322–1334

    Article  CAS  PubMed  Google Scholar 

  • Rubiales D, Niks RE (1995) Characterization of Lr34, a major gene conferring nonhypersensitive resistance to wheat leaf rust. Plant Dis 79:1208–1212

    Google Scholar 

  • Rubiales D, Moreno MT, Sillero JC (2005) Search for resistance to crenata broomrape (Orobanche crenata) in pea germplasm. Genet Res Crop Evol 52:853–861

    Article  Google Scholar 

  • Rubiales D, Fernández-Aparicio M, Moral A, Barilli E, Sillero JC, Fondevilla S (2009) Disease resistance in pea (Pisum sativum L.) types for autumn sowings in Mediterranean environments—a review. Czech J Genet Plant Breed 45:135–142

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbour Laboratory Press, New York

    Google Scholar 

  • Sato S, Isobe S, Asamizu E, Ohmido N, Kataoka R, Nakamura Y, Kaneko T, Sakurai N, Okumura K, Klimenko I, Sasamoto S, Wada T, Watanabe A, Kohara M, Fujishiro T, Tabata S (2005) Comprehensive structural analysis of the genome of red clover (Trifolium pratense L.). DNA Res 12:301–336

    Article  CAS  PubMed  Google Scholar 

  • Sillero JC, Moreno MT, Rubiales D (2000) Characterization of new sources of resistance to Uromyces viciae-fabae in a germplasm collection of Vicia faba. Plant Pathol 49:389–395

    Article  Google Scholar 

  • Sillero JC, Fondevilla S, Davidson J, Vaz Patto MC, Warketin TD, Thomas J, Rubiales D (2006) Screening techniques and sources of resistance to rusts and mildews in grain legumes. Euphytica 147:255–267

    Article  Google Scholar 

  • Singh R, Ram H (2001) Inheritance of days to flowering and rust resistance in peas. Res Crops 2:414–418

    Google Scholar 

  • Singh SJ, Sokhi SS (1980) Evaluation of pea cultivars to Uromyces viciae-fabae. Plant Dis 64:671–672

    Google Scholar 

  • Singh R, Kazi M, Huerta-Espino J (1998) Lr46, a gene conferring slow rusting resistance to leaf rust in wheat. Phytopathology 88:890–894

    Article  CAS  PubMed  Google Scholar 

  • Singh RA, De RK, Chaudhary RG (2004) Influence of spray time of mancozeb on pea rust caused by Uromyces viciae-fabae. Indian J Agric Sci 74:502–504

    Google Scholar 

  • Stackman EC, Stewart DM, Loegering WQ (1962) Identification of physiologic races of Puccinia graminis var. tritici. USDA, Agricultural Research Service. E617, Washington

    Google Scholar 

  • Stavely JR, Steadman JR, McMillan RT (1989) New pathogenic variability in Uromyces appendiculatus in North America. Plant Dis 73:428–432

    Article  Google Scholar 

  • Subrahmanyam P, McDonald D, Reddy LJ, Nigam SN, Smith DH (1993) Origin and utilization of rust resistance in groundnut. In: Jacobs T, Parlevliet JE (eds) Durability of disease resistance. Kluver Academic Publishers, Dordrecht, pp 147–158

    Google Scholar 

  • Tar’an B, Warkentin T, Somers DJ, Miranda D, Vandenberg A, Balde S, Woods S, Bing D, Xue A, DeKoeyer D, Penner G (2003) Quantitative trait loci for lodging resistance. Plant height and partial resistance to mycosphaerella blight in field pea (Pisum sativum L.). Theor Appl Genet 107:1482–1491

    Article  PubMed  Google Scholar 

  • Tauz D, Renz M (1984) Simple sequences are ubiquitous repetitive components of eukaryotic genomes. Nucleic Acids Res 12:4127–4138

    Article  Google Scholar 

  • Timmermann-Vaughan GM, Frew TJ, Butler R, Murray S, Gilpin M, Falloon K, Johnston P, Lakeman MB, Russell AC, Khan T (2004) Validation of quantitative trait loci for Ascochyta blight resistance in pea (Pisum sativum L.), using populations from two crosses. Theor Appl Genet 109:1620–1631

    Article  Google Scholar 

  • Torres AM, Weeden NF, Martín A (1993) Linkage among isozyme, RFLP and RAPD markers in Vicia faba. Theor Appl Genet 85:937–945

    Article  CAS  Google Scholar 

  • Vijayalakshmi S, Yadav K, Kushwaha C, Sarode SB, Srivastava CP, Chand R, Singh BD (2005) Identification of RAPD markers linked to the rust (Uromyces fabae) resistance gene in pea (Pisum sativum). Euphytica 144:265–274

    Article  CAS  Google Scholar 

  • EPPO Standards Pea http://archives.eppo.org/EPPOStandards/PP2_GPP/pp2-14-e.doc. Visited 13 November 2009

  • Wang S, Basten CJ, Gaffney P, Zeng ZB (2005) Windows QTL cartographer version 2.5. Statistical genetics. North Carolina State University, Raleigh

    Google Scholar 

  • Weeden NF, Ellis THN, Timmerman-Vaughan GM, Swiecicki WK, Rozov SM, Berdnikov VA (1998) A consensus likage map for Pisum sativum. Pisum Genetics 30:1–4

    Google Scholar 

  • Xue AG, Warkentin TD (2001) Reaction of field pea varieties to three isolates of Uromyces fabae. Plant Sci 82:253–255

    Google Scholar 

Download references

Acknowledgments

We thank N. Gutiérrez, C. Avila, L. Madrid and G. Lara Santos for good advice and technical support in the laboratory. Authors are greatly indebted to Spanish AGL2008-01239 and European Union FP6-2002-FOOD-1-506223 projects for financial support.

Author information

Authors and Affiliations

  1. Institute for Sustainable Agriculture, CSIC, Avda Menendez Pidal s/n, Apdo. 4080, 14080, Córdoba, Spain

    E. Barilli & D. Rubiales

  2. Department of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, 10000, Zagreb, Croatia

    Z. Satovic

  3. IFAPA, Centro Alameda del Obispo, Avda. Menendez Pidal s/n, Apdo. 3092, 14080, Córdoba, Spain

    A. M. Torres

Authors
  1. E. Barilli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Z. Satovic
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Rubiales
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. M. Torres
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. Barilli.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Barilli, E., Satovic, Z., Rubiales, D. et al. Mapping of quantitative trait loci controlling partial resistance against rust incited by Uromyces pisi (Pers.) Wint. in a Pisum fulvum L. intraspecific cross. Euphytica 175, 151–159 (2010). https://doi.org/10.1007/s10681-010-0141-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-010-0141-z

Keywords

Search

Navigation