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Identification and mapping of quantitative trait loci for leaf rust resistance derived from a tetraploid wheat Triticum dicoccum accession

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Abstract

The Triticum turgidum ssp. dicoccum (2n = 4x = 28) accession MG5323 showed a useful level of resistance to leaf rust disease. A segregating population of 110 recombinant inbred lines (RILs), derived from a cross between cv Latino (T. turgidum spp. durum), susceptible to leaf rust, and MG5323 was evaluated for reactions of seedlings to two different Puccinia triticina isolates. Genotyping of the RILs was performed with different molecular markers (SSR, EST-SSR and SNP), leading to the construction of a linkage map containing 10,840 loci covering 14 chromosomes, with an average marker density of 0.22 cM/marker. Linkage analysis allowed the identification of three different regions significantly associated with leaf rust resistance, with MG5323 contributing the resistant alleles. A major resistance gene was detected on the short arm of chromosome 1B, explaining a total phenotypic variation ranging from 41.37 to 49.51 %. Two additional minor resistance genes located on chromosome 7B explained a phenotypic variation ranging between 17.77 and 25.81 %. No obvious positional relationships were observed when the map position of the genes was compared with those of other previously identified wheat leaf rust resistance genes, suggesting that new resistance sources to leaf rust were identified in the tetraploid background. A significant positive epistatic effect was detected between quantitative trait loci (QTLs) for each trait, indicating that different QTLs contribute different degrees of resistance. Analysis of the leaf rust responses of the RILs demonstrated that only lines bearing resistant alleles at both loci showed effective leaf rust resistance, indicating that the genes identified behave as complementary genes.

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Authors and Affiliations

  1. Consiglio per la ricerca e la sperimentazione in agricoltura, Genomics Research Centre, Fiorenzuola d’Arda, Piacenza, Italy

    F. Desiderio, D. Guerra, L. Cattivelli & G. Vale’

  2. CSIC, Institute for Sustainable Agriculture, Córdoba, Spain

    D. Rubiales

  3. Department of Environmental and Agro-Forestry Biology and Chemistry, University of Bari, Bari, Italy

    L. Piarulli, R. Simeone & A. Blanco

  4. Consiglio per la ricerca e la sperimentazione in agricoltura, Cereal Quality Research Unit (QCE), Rome, Italy

    M. Pasquini

  5. Consiglio per la ricerca e la sperimentazione in agricoltura, Cereal Research Centre, Foggia, Italy

    A. M. Mastrangelo

  6. Consiglio per la ricerca e la sperimentazione in agricoltura, Rice Research Unit, S.S. 11 to Torino, Km 2.5, 13100, Vercelli, Italy

    G. Vale’

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  1. F. Desiderio
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  2. D. Guerra
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  3. D. Rubiales
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  4. L. Piarulli
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  6. A. M. Mastrangelo
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  7. R. Simeone
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  8. A. Blanco
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  9. L. Cattivelli
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Correspondence to F. Desiderio.

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11032_2014_186_MOESM1_ESM.tif

Fig S1 Infection types produced by the parental lines against different P. triticina isolates. a) infection types produced by Latino and MG5323 challenged with five different leaf rust isolates; b) pictures of the leaf rust symptoms on Latino (LT) and MG5323 (MG) leaves obtained with isolates VMC03 and 12766 used for screening the RILs at 15 days after inoculation. (TIFF 2286 kb)

Fig S2 Pairwise recombination fractions and LOD scores for Latino × MG5323 linkage map. (TIFF 7119 kb)

11032_2014_186_MOESM3_ESM.tif

Fig S3 Frequency distribution of phenotypic reactions of RILs to leaf rust isolates VMC03 and 12766 expressed as infection type (IT) or as relative disease severity classes (RDS) considering two major classes: resistant (IT values between 0 and 5; RDS values from 0 to 50) and susceptible (IT values from 6 to 9; RDS values from 51 to 130). (TIFF 584 kb)

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Desiderio, F., Guerra, D., Rubiales, D. et al. Identification and mapping of quantitative trait loci for leaf rust resistance derived from a tetraploid wheat Triticum dicoccum accession. Mol Breeding 34, 1659–1675 (2014). https://doi.org/10.1007/s11032-014-0186-0

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