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Mapping of quantitative trait loci for partial resistance to Mycosphaerella pinodes in pea (Pisum sativum L.), at the seedling and adult plant stages

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Abstract

The inheritance of resistance to Ascochyta blight, an economically important foliar disease of field pea (Pisum sativum L.) worldwide, was investigated. Breeding resistant pea varieties to this disease, caused by Mycosphaerella pinodes, is difficult due to the availability of only partial resistance. We mapped and characterized quantitative trait loci (QTLs) for resistance to M. pinodes in pea. A population of 135 recombinant inbred lines (RILs), derived from the cross between DP (partially resistant) and JI296 (susceptible), was genotyped with morphological, RAPD, SSR and STS markers. A genetic map was elaborated, comprising 206 markers distributed over eight linkage groups and covering 1,061 cM. The RILs were assessed under growth chamber and field conditions at the seedling and adult plant stages, respectively. Six QTLs were detected at the seedling stage, which together explained up to 74% of the variance. Ten QTLs were identified at the adult plant stage in the field, and together these explained 56.6–67.1% of the variance, depending on the resistance criteria and the organ considered. Four QTLs were detected under both growth chamber and field conditions, suggesting they were not plant-stage dependent. Three QTLs for flowering date and three QTLs for plant height were also identified in the RIL population, some of which co-located with QTLs for resistance. The relationship between QTLs for resistance to M. pinodes, plant height and flowering date is discussed.

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Acknowledgements

This work was supported by the Union Nationale Interprofessionnelle des Plantes riches en Protéines (UNIP). We would like to thank L. Gervais and M.-L. Pilet-Nayel for their helpful comments on the manuscript. We also thank J.-M. Abélard, R. Horvais, R. Menant and J. Poisson for technical assistance.

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  1. Institut National de la Recherche Agronomique (INRA), UMR INRA ENSAR d’Amélioration des Plantes et Biotechnologies Végétales, Domaine de la Motte au Vicomte, BP 35327, 35653, Le Rheu Cedex, France

    S. Prioul, A. Frankewitz, G. Deniot, G. Morin & A. Baranger

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  1. S. Prioul
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  2. A. Frankewitz
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  3. G. Deniot
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  4. G. Morin
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  5. A. Baranger
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Correspondence to A. Baranger.

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Communicated by H.C. Becker

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Prioul, S., Frankewitz, A., Deniot, G. et al. Mapping of quantitative trait loci for partial resistance to Mycosphaerella pinodes in pea (Pisum sativum L.), at the seedling and adult plant stages. Theor Appl Genet 108, 1322–1334 (2004). https://doi.org/10.1007/s00122-003-1543-2

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  • DOI: https://doi.org/10.1007/s00122-003-1543-2

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