Skip to main content
SpringerLink
Log in

Selecting genetic transformants of indica and indica-derived rice cultivars using bispyribac sodium and a mutated ALS gene

  • Original Paper
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Bispyribac sodium (BS), a pyrimidinyl carboxy herbicide, is a well-known inhibitor of acetolactate synthase (ALS) activity. ALS is an enzyme in the biosynthetic pathway for branched-chain amino acids. A mutant form of rice ALS (OsmALS [W548L/S627I]) that confers resistance to BS can be used as an in vitro selection marker gene for plant transformation. Since indica and indica-derived cultivars are thought to have lower BS sensitivity than japonica rice, the application of BS as a selectable reagent for genetic transformation in indica and indica-derived cultivars is more challenging than for japonica cultivars. In this study, callus and seedlings of eight different rice cultivars (five indica-derived cultivars, two indica cultivars and one japonica cultivar) were tested for BS sensitivity. Our study indicates for the first time that callus shows a higher sensitivity to BS than seedlings in indica and indica-derived cultivars. We used BS with OsmALS [W548L/S627I] to select transformed calli, and transgenic rice plants from indica and indica-derived cultivars were successfully obtained.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  • Andersson M, Trifonova A, Andersson AB, Johansson M, Bülow L, Hofvander P (2003) A novel selection system for potato transformation using a mutated AHAS gene. Plant Cell Rep 22(4):261–267

    Article  CAS  PubMed  Google Scholar 

  • Charest PJ, Hattori J, DeMoor J, Iyer VN, Miki BL (1990) In vitro study of transgenic tobacco expressing Arabidopsis wild type and mutant acetohydroxyacid synthase genes. Plant Cell Rep 8:643–646

    Article  CAS  Google Scholar 

  • Chipman D, Barak Z, Schloss JV (1998) Biosynthesis of 2-aceto-2-hydroxy acids: acetolactate synthases and acetohydroxyacid synthases. Biochem Biophys Acta 1385(2):401–419

    CAS  PubMed  Google Scholar 

  • Corbett CA, Tardif FJ (2006) Detection of resistance to acetolactate synthase inhibitors in weeds with emphasis on DNA-based techniques: a review. Pest Manag Sci 62(7):584–597

    Article  CAS  PubMed  Google Scholar 

  • Datta K, Datta SK (2006) Indica rice (Oryza sativa, BR29 and IR64). Methods Mol Biol 343:201–212

    PubMed  Google Scholar 

  • Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19(6):1349

    Article  CAS  PubMed  Google Scholar 

  • Gabard JM, Charest PJ, Iyer VN, Miki BL (1989) Cross-resistance to short residual sulfonylurea herbicides in transgenic tobacco plants. Plant Physiol 91(2):574–580

    Article  CAS  PubMed  Google Scholar 

  • Hanzawa S, Kobayashi Y (1999) Weed control before permanent water stage of combine sowing culture of rice. Tohoku Agric Res 52:65–66

    Google Scholar 

  • Kawai K, Kaku K, Izawa N, Shimizu T, Fukuda A, Tanaka Y (2007a) A novel mutant acetolactate synthase gene from rice cells, which confers resistance to ALS-inhibiting herbicides. J Pestic Sci 32:89–98

    Article  CAS  Google Scholar 

  • Kawai K, Kaku K, Izawa N, Shimizu T, Fukuda A, Tanaka Y, Shimizu T (2007b) Functional analysis of transgenic rice plants expressing a novel mutated ALS gene of rice. J Pestic Sci 32:385–392

    Article  CAS  Google Scholar 

  • Kobayashi M, Yokoyama M, Watanabe O, Sadohara H, Wada N (1995) KIH-2023, a new post-emergence herbicide in rice (Oryza sativa). 15th Asian-Pacific Weed Sci Soc Conf Proc I(A), Kyoto, pp 221–226

  • Li Z, Hayashimoto A, Murai N (1992) A sulfonylurea herbicide resistance gene from Arabidopsis thaliana as a new selectable marker for production of fertile transgenic rice plants. Plant Physiol 100(2):662–668

    Article  CAS  PubMed  Google Scholar 

  • Lin YJ, Zhang Q (2005) Optimising the tissue culture conditions for high efficiency transformation of indica rice. Plant Cell Rep 23(8):540–547

    Article  CAS  PubMed  Google Scholar 

  • Lin J, Zhou B, Yang Y, Mei J, Zhao X, Guo X, Huang X, Tang D, Liu X (2009) Piercing and vacuum infiltration of the mature embryo: a simplified method for Agrobacterium-mediated transformation of indica rice. Plant Cell Rep 28(7):1065–1074

    Article  CAS  PubMed  Google Scholar 

  • Nishimura A, Aichi I, Matsuoka M (2006) A protocol for Agrobacterium-mediated transformation in rice. Nat Protoc 1(6):2796–2802

    Article  CAS  PubMed  Google Scholar 

  • Ogawa T, Kawahigashi H, Toki S, Hand Y (2008) Efficient transformation of wheat by using a mutated rice acetolactate synthase gene as a selectable marker. Plant Cell Rep 27:1325–1331

    Article  CAS  PubMed  Google Scholar 

  • Ohno S, Watanabe O, Hanai R, Endou Y, Fujiwara S (2008) Safety of several forage rice varieties to bispyribac-sodium. Jpn J Crop Sci 76(1):22

    Google Scholar 

  • Okuzaki A, Toriyama K (2004) Chimeric RNA/DNA oligonucleotide-directed gene targeting in rice. Plant Cell Rep 22(7):509–512

    Article  CAS  PubMed  Google Scholar 

  • Okuzaki A, Shimizu T, Kaku K, Kawai K, Toriyama K (2007) A novel mutated acetolactate synthase gene conferring specific resistance to pyrimidinyl carboxy herbicides in rice. Plant Mol Biol 64(1–2):219–224

    Article  CAS  PubMed  Google Scholar 

  • Osakabe K, Endo M, Kawai K, Nishizawa Y, Ono K, Abe K, Ishikawa Y, Nakamura H, Ichikawa H, Nishimura S, Shimizu T, Toki S (2005) The mutant form of acetolactate synthase genomic DNA from rice is an efficient selectable marker for genetic transformation. Mol Breed 16:313–320

    Article  CAS  Google Scholar 

  • Ramesh M, Murugiah V, Gupta AK (2009) Efficient in vitro plant regeneration via leaf base segments of indica rice (Oryza sativa L.). Indian J Exp Biol 47(1):68–74

    CAS  PubMed  Google Scholar 

  • Ray K, Jagannath A, Gangwani SA, Burma PK, Pental D (2004) Mutant acetolactate synthase gene is an efficient in vitro selectable marker for the genetic transformation of Brassica juncea (oilseed mustard). J Plant Physiol 161(9):1079–1083

    Article  CAS  PubMed  Google Scholar 

  • Shimizu M, Goto M, Hanai M, Shimizu T, Izawa N, Kanamoto H, Tomizawa K, Yokota A, Kobayashi H (2008) Selectable tolerance to herbicides by mutated acetolactate synthase genes integrated into the chloroplast genome of tobacco. Plant Physiol 147:1976–1983

    Article  CAS  PubMed  Google Scholar 

  • Shimizu T, Nakayama I, Nagayama K, Miyazawa T, Nezu Y (2002) Acetolactate synthase inhibitors. In: Boger P, Wakabayashi K, Hirai K (eds) Herbicide classes in development: mode of action-targets-genetics engineering-chemistry. Berlin, New York, pp 1–41

  • Shimizu T, Kaku K, Kawai K, Miyazawa T, Tanaka Y (2005) Molecular characterization of acetolactate synthase in resistant weeds and crops. In: Clark JM, Ohkawa H (eds) ACS symposium series 899: environmental fate and safety management of agrochemicals. American Chem Soc, Washington, DC, pp 255–271

  • Singh BK, Shaner DL (1995) Biosynthesis of branched chain amino acids: from test tube to field. Plant Cell 7:935–944

    Article  CAS  PubMed  Google Scholar 

  • Tanaka H, Kayano T, Ugaki M, Shiobara F, Onodera H, Ono K, Tagiri A, Nishizawa Y, Shibuya N (2005) Ultra-fast transformation technique for monocotyledons. U.S. Patent Appl 20050138694

  • Toki S (1997) Rapid and efficient Agrobacterium-mediated transformation in rice. Plant Mol Biol report 15:16–21

    Article  CAS  Google Scholar 

  • Tougou M, Yamagishi N, Furutani N, Kaku K, Shimizu T, Takahata Y, Sakai J, Kanematsu S, Hidaka S (2009) The application of the mutated acetolactate synthase gene from rice as the selectable marker gene in the production of transgenic soybeans. Plant Cell Rep 28:769–776

    Article  CAS  PubMed  Google Scholar 

  • Tranel PJ, Wright TR (2002) Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Sci 50:700–712

    Article  CAS  Google Scholar 

  • Uchino A (2002) Rapid diagnosis of sulfonylurea-resistance biotypes based on acetolactate synthase activity in the lowland weeds of Japan. J Weed Sci Tech 47:197–201

    CAS  Google Scholar 

  • Wakasa Y, Ozawa K, Takaiwa F (2007) Agrobacterium-mediated transformation of a low glutelin mutant of ‘Koshihikari’ rice variety using the mutated-acetolactate synthase gene derived from rice genome as a selectable marker. Plant Cell Rep 26(9):1567–1573

    Article  CAS  PubMed  Google Scholar 

  • Watanabe O, Yokoyama M, Fujita S, Miyazaki T, Wada N (2003) The development of rice herbicide and growth retardant, bispyribac-sodium. J Weed Sci Tech 48:24–30

    CAS  Google Scholar 

  • Wünn J, Klöti A, Burkhardt PK, Biswas GC, Launis K, Iglesias VA, Potrykus I (1996) Transgenic indica rice breeding line IR58 expressing a synthetic cryIA(b) gene from Bacillus thuringiensis provides effective insect pest control. Biotechnology 14(2):171–176

    Article  PubMed  Google Scholar 

  • Yano M, Sasaki T (1997) Genetic and molecular dissection of quantitative traits in rice. Plant Mol Biol 35(1–2):145–153 Review

    Article  CAS  PubMed  Google Scholar 

  • Yu Q, Abdallah I, Han H, Owen M, Powles S (2009) Distinct non-target site mechanisms endow resistance to glyphosate, ACCase and ALS-inhibiting herbicides in multiple herbicide-resistant Lolium rigidum. Planta 230(4):713–723

    Article  CAS  PubMed  Google Scholar 

  • Zhang Y, Yin X, Yang A, Li G, Zhang J (2005) Stability of inheritance of transgenes in maize (Zea mays L.) lines produced using different transformation methods. Euphytica 144:11–22

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors wish to thank Dr. F. Abe (National Institute of Crop Science) for useful advice. This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics for Agricultural Innovation, GMA0002).

Author information

Authors and Affiliations

  1. National Institute of Crop Science, Kannondai 2-1-18, Tsukuba, Ibaraki, 305-8518, Japan

    Yojiro Taniguchi, Motoshige Kawata, Ikuo Ando & Masahiro Ohshima

  2. Life Science Research Institute, Kumiai Chemical Industry Co. Ltd, 3360 Kamo, Kikugawa, Shizuoka, 439-0031, Japan

    Tsutomu Shimizu

Authors
  1. Yojiro Taniguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Motoshige Kawata
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ikuo Ando
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tsutomu Shimizu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Masahiro Ohshima
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masahiro Ohshima.

Additional information

Communicated by K. Toriyama.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Taniguchi, Y., Kawata, M., Ando, I. et al. Selecting genetic transformants of indica and indica-derived rice cultivars using bispyribac sodium and a mutated ALS gene. Plant Cell Rep 29, 1287–1295 (2010). https://doi.org/10.1007/s00299-010-0915-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-010-0915-8

Keywords

Search

Navigation