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Agrobacterium-mediated transformation of a low glutelin mutant of ‘Koshihikari’ rice variety using the mutated-acetolactate synthase gene derived from rice genome as a selectable marker

  • Genetic Transformation and Hybridization
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An Erratum to this article was published on 15 March 2008

Abstract

We have developed an efficient rice transformation system that uses only rice genome-derived components. The transgenic ‘Koshihikari’ rice, low-glutelin mutant a123, is capable of accumulating large amounts of bioactive peptides in the endosperm. Agrobacterium-mediated transformation using the mutated-acetolactate synthase (mALS) gene expressed under the control of the callus-specific promoter (CSP) as a selectable marker was used to introduce GFP and an anti-hypertensive hexapeptide into ‘Koshihikari’ a123. The CSP:mALS gene cassette confers pyrimidinyl carboxy herbicide resistance to transgenic rice callus, but is not expressed in regenerated plants. Transformation efficiency of transgenic rice line a123 was improved from about 10% to about 30% by modifying callus induction, callus selection and regeneration media conventionally used for rice tissue culture.

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Acknowledgments

The authors wish to thank Drs. C. Genetello and M. Karimi (Ghent University, Ghent, Belgium) for kindly providing the vectors pHGW, pHm24GW, and pHm43GW. This work was supported by a ‘Development and Demonstration of Crop Genomic Breeding Technology’ project grant to F. Takaiwa.

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Authors and Affiliations

  1. Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Kannondai 3-1-3, Tsukuba, Ibaraki, 305-8602, Japan

    Yuhya Wakasa & Fumio Takaiwa

  2. Department of Low-Temperature Sciences, National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Toyohira-ku, Sapporo, Hokkaido, 062-8555, Japan

    Kenjirou Ozawa

Authors
  1. Yuhya Wakasa
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  2. Kenjirou Ozawa
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  3. Fumio Takaiwa
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Correspondence to Fumio Takaiwa.

Additional information

Communicated by H. Ebinuma.

An erratum to this article can be found at http://dx.doi.org/10.1007/s00299-008-0533-x

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Wakasa, Y., Ozawa, K. & Takaiwa, F. Agrobacterium-mediated transformation of a low glutelin mutant of ‘Koshihikari’ rice variety using the mutated-acetolactate synthase gene derived from rice genome as a selectable marker. Plant Cell Rep 26, 1567–1573 (2007). https://doi.org/10.1007/s00299-007-0373-0

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  • DOI: https://doi.org/10.1007/s00299-007-0373-0

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