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Chimeric RNA/DNA oligonucleotide-directed gene targeting in rice

  • Genetics and Genomics
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Abstract

Site-specific mutagenesis in a rice genome was obtained by introducing chimeric RNA/DNA oligonucleotides (COs) by means of particle bombardment. Three COs were designed to target the independent codons for Pro-171, Trp-548 and Ser-627 of the endogenous rice acetolactate synthase (ALS) gene so it would confer resistance to ALS-inhibiting herbicides. Sequencing of the ALS gene of herbicide-resistant plants demonstrated that the ALS sequence was modified in a site-specific fashion. The efficiency of gene conversion mediated by COs was estimated to be 1×10-4. These results demonstrate that CO-directed gene targeting is feasible in rice.

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Abbreviations

ALS :

Acetolactate synthase

BS :

Bispyribac-sodium

Cf :

Chlorsulfuron

CO :

Chimeric RNA/DNA oligonucleotide

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Acknowledgements

We are grateful to the Kumiai Chemical Industry Co, Shizuoka, Japan for the gifts of BS and valuable comments.

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Authors and Affiliations

  1. Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, 981-8555, Sendai, Japan

    A. Okuzaki & K. Toriyama

Authors
  1. A. Okuzaki
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  2. K. Toriyama
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Correspondence to K. Toriyama.

Additional information

Communicated by H. Uchimiya

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Okuzaki, A., Toriyama, K. Chimeric RNA/DNA oligonucleotide-directed gene targeting in rice. Plant Cell Rep 22, 509–512 (2004). https://doi.org/10.1007/s00299-003-0698-2

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  • DOI: https://doi.org/10.1007/s00299-003-0698-2

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