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Rapid and efficientAgrobacterium-mediated transformation in rice

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Abstract

An improved protocol forAgrobacterium-mediated transformation of rice is described. The procedure permitted the regeneration of transgenic plants from callus in only two months.

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References

  • Chilton, M.-D., T.C. Currier, S.K. Farrand, A.J. Bendich, M.P. Gordon and E.W. Nester. 1974.Agrobacterium tumefaciens, DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc. Natl. Acad. Sci. USA 71:3672–3676.

    Article  PubMed  CAS  Google Scholar 

  • Chu, C.C., C.S. Wang, C.C. Sun, C. Hsu, K.C. Yin, C.Y. Chu. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci. Sinica 18:659–668.

    Google Scholar 

  • Christensen, A.H., R.A. Sharrock, P.H. Quail. 1992. Maize polyubiquitin genes: Structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Mol. Biol. 18:675–689.

    Article  PubMed  CAS  Google Scholar 

  • Hajdukiewicz, P., Z. Svav and P. Maliga. 1974. The small, versatilepPZP family ofAgrobacterium binary veectors for plant transformation. Plant Mol. Biol. 25:989–994.

    Article  Google Scholar 

  • Hiei, Y., S. Ohta, T. Komari and T. Kumashiro. 1994. Efficient transformation of rice (Oryza sativa L.) mediated byAgrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J. 6:271–282.

    Article  PubMed  CAS  Google Scholar 

  • Hood, E.E., G.L. Helmer, R.T. Fraley and M.D. Chilton. 1986. The hypervirulence ofAgrobacterium tumefaciens, A281 is encoded in a region pTiBo542 outside of T-DNA. J Bacteriol. 168:1291–1301.

    PubMed  CAS  Google Scholar 

  • Krebbers, E., J. Seurinck, L. Herdies, A.R. Cashmore, and M.P. Timko. 1988. Four genes in two diverged subfamilies encode the ribulose 1,5-bisphosphate carboxylase small subunit polypeptides ofArabidopsis thaliana. Plant Mol. Biol. 11:746–759.

    Article  Google Scholar 

  • Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497.

    Article  CAS  Google Scholar 

  • Rashid, H., S. Yokoi, K. Toriyama and K. Hinata. 1996. Transgenic plant production mediated byAgrobacterium inIndica rice. Plant Cell Rep. 15:727–730.

    Article  CAS  Google Scholar 

  • Yamashita, S., H. Ichikawa, Y. Itoh and Y. Ohashi. 1995. Development of new binary vectors stably maintained inAgrobacterium and: their utilization to plant transfromation. (in Japanese)Breeding Science 45 suppl. 1:56.

    Google Scholar 

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Correspondence to Seiichi Toki.

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Toki, S. Rapid and efficientAgrobacterium-mediated transformation in rice. Plant Mol Biol Rep 15, 16–21 (1997). https://doi.org/10.1007/BF02772109

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  • DOI: https://doi.org/10.1007/BF02772109

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