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A novel selection system for potato transformation using a mutated AHAS gene

  • Genetic Transformation and Hybridization
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Abstract

Acetohydroxyacid synthase (AHAS) is the target enzyme for a number of herbicides. A S653N mutation in the AHAS gene results in an increased tolerance to imidazolinone herbicides. We have investigated the use of the mutated gene as selection gene for potato transformation. This resulted in a transformation system with a very high transformation frequency and low rate of escapes. The mutated AHAS gene was introduced into transformed potato together with a β-glucuronidase (GUS) gene. Selection on 0.5 μM Imazamox yielded GUS expression in 93–100% of regenerated shoots. Furthermore the mutated AHAS gene was used as selection gene for production of high-amylopectin potato lines. The high transformation frequency was verified and potato lines with the desirable starch quality were obtained.

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Fig. 1.
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Fig. 4A, B.

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Abbreviations

ABA :

Abscisic acid

AHAS :

Acetohydroxyacid synthase

BAP :

6-Benzylaminopurine

2,4-D :

2, 4-Dichlorophenoxyacetic acid

GA 3 :

Gibberellic acid

GBSS:

Granule bound starch synthase

GUS:

β-Glucuronidase

MS medium :

Murashige and Skoog medium

NAA :

α-Naphthaleneacetic acid

nos :

Nopaline synthase

OCS :

Octopine synthase

PCR:

Polymerase chain reaction

X-gluc :

5-Bromo-4-chloro-3-indolyl-beta-d-glucuronic acid

YEB :

Yeast extract broth

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Acknowledgement

We would like to thank the researchers at BASF Plant Science in RTP, North Carolina for the mutated AHAS gene.

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Authors and Affiliations

  1. Plant Science Sweden AB, Herman Ehles Väg 2–4, 268 31, Svalöv, Sweden

    M. Andersson, A. Trifonova, A.-B. Andersson, M. Johansson & P. Hofvander

  2. Pure and Applied Biochemistry, Lund University, PO Box 124, 221 00, Lund, Sweden

    M. Andersson & L. Bülow

  3. Department of Plant Biology, Swedish University of Agricultural Sciences, PO Box 7080, 750 07, Uppsala, Sweden

    P. Hofvander

Authors
  1. M. Andersson
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  2. A. Trifonova
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  3. A.-B. Andersson
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  4. M. Johansson
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  5. L. Bülow
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  6. P. Hofvander
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Corresponding author

Correspondence to M. Andersson.

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Communicated by R. Schmidt

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Andersson, M., Trifonova, A., Andersson, AB. et al. A novel selection system for potato transformation using a mutated AHAS gene. Plant Cell Rep 22, 261–267 (2003). https://doi.org/10.1007/s00299-003-0684-8

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  • DOI: https://doi.org/10.1007/s00299-003-0684-8

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