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Mapping of the novel powdery mildew resistance gene Pm2Mb from Aegilops biuncialis based on ph1b-induced homoeologous recombination

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Abstract

Key message

A novel powdery mildew resistance gene Pm2Mb from Aegilops biuncialis was transferred into common wheat and mapped to chromosome 2MbL bin FL 0.49–0.66 by molecular cytogenetic analysis of 2Mb recombinants.

Abstract

Aegilops biuncialis, a wild relative of common wheat, is highly resistant to powdery mildew. Previous studies identified that chromosome 2Mb in Chinese Spring (CS)-Ae. biuncialis 2Mb disomic addition line TA7733 conferred high resistance to powdery mildew, and the resistance gene was temporarily designated as Pm2Mb. In this study, a total of 65 CS-Ae. biuncialis 2Mb recombinants were developed by ph1b-induced homoeologous recombination and they were grouped into 12 different types based on the presence of different markers of 2Mb-specificity. Segment sizes and breakpoints of each 2Mb recombinant type were further characterized using in situ hybridization and molecular marker analyses. Powdery mildew responses of each type were assessed by inoculation of each 2Mb recombinant-derived F2 progenies using the isolate E05. Combined analyses of in situ hybridization, molecular markers and powdery mildew resistance data of the 2Mb recombinants, the gene Pm2Mb was cytologically located to an interval of FL 0.49–0.66 in the long arm of 2Mb, where 19 2Mb-specific markers were located. Among the 65 2Mb recombinants, T-11 (T2DS.2DL-2MbL) and T-12 (Ti2DS.2DL-2MbL-2DL) contained a small 2MbL segment harboring Pm2Mb. Besides, a physical map of chromosome 2Mb was constructed with 70 2Mb-specific markers in 10 chromosomal bins and the map showed that submetacentric chromosome 2Mb of Ae. biuncialis was rearranged by a terminal intrachromosomal translocation. The newly developed 2Mb recombinants with powdery mildew resistance, the 2Mb-specific molecular markers and the physical map of chromosome 2Mb will benefit wheat disease breeding as well as fine mapping and cloning of Pm2Mb.

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Data availability

All data generated or analyzed during this study are included in this published article and its supplementary information files. Common wheat CS, Ae. comosa accession TA2102, CS-Ae. biuncialis 2Mb disomic addition line TA7733 and CS ph1b mutant TA3809 are maintained by Wheat Genetic Resource Center at Kansas State University, USA, and Henan Agricultural University, China. Sixty-five CS-Ae. biuncialis 2Mb recombinants maintained by Henan Agricultural University, China. All plant materials in this study are available for research purposes inside and outside the country from the corresponding author by request.

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Acknowledgements

We would like to thank Dr. Pengtao Ma (College of Life and Sciences, Yantai University) for providing Bgt isolate E05.

Funding

This research was financially supported by the National Natural Science Foundation of China (Nos. 31801361 and 31971887), the Scientific and Technological Research Project of Henan Province of China (No. 212102110059) and the Topnotch Talents of Henan Agricultural University (30500939).

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Authors and Affiliations

  1. The State Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, Henan, China

    Wenqiang Men, Ziwei Fan, Chao Ma, Yue Zhao, Chaoli Wang, Xiubin Tian, Qifan Chen, Jingnan Miao, Jinqiu He, Jiajun Qian, Huanhuan Li & Wenxuan Liu

  2. Department of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD, 57007, USA

    Sunish K. Sehgal

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  1. Wenqiang Men
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  2. Ziwei Fan
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  3. Chao Ma
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  4. Yue Zhao
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  6. Xiubin Tian
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Contributions

WXL and HHL: conceived the research. WQM, HHL and ZWF: performed the research. CM, YZ, CLW, XBT, QFC, JNM, JQH and SKS: participated in the preparation of the reagents and materials used in this study. HHL, WXL and SKS: wrote the paper.

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Correspondence to Huanhuan Li or Wenxuan Liu.

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Men, W., Fan, Z., Ma, C. et al. Mapping of the novel powdery mildew resistance gene Pm2Mb from Aegilops biuncialis based on ph1b-induced homoeologous recombination. Theor Appl Genet 135, 2993–3003 (2022). https://doi.org/10.1007/s00122-022-04162-4

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