Abstract
Key message
Using the ph1b mutant, the recombination frequency between the homoeologous region of 2B and 2G was significantly increased. By this, we narrowed Pm6 to a 0.9 Mb physical region.
Abstract
The powdery mildew (Pm) resistance gene Pm6 from Triticum timopheevii (2n = 48, AAGG) was mapped to the long arm of chromosome 2G and introduced into common wheat in the form of 2B–2G introgressions. The introgression line IGV1-465 has the shortest 2G segment, which is estimated 37 Mb in size when referring to 2BL genome reference of Chinese Spring (CS). The further fine mapping of Pm6 was impeded by the inhibition of allogeneic chromosome recombination between 2B and 2G in the Pm6 region. In the present study, to overcome 2B/2G recombination suppression, a ph1b-based strategy was employed to produce introgressions with reduced 2G fragments for the fine mapping of Pm6. IGV1-465 was crossed and backcrossed to the CSph1b mutant to produce plants with increased 2B/2G chromosome pairing frequency at the Pm6 region. A total of 182 allogeneic recombinants were obtained through two-round screening, i.e., first round of screening of 820 BC1F2:3 progenies using the flanking markers CIT02g-14/CIT02g-19 and second round of screening of 642 BC1F2:4 progenies using the flanking markers CIT02g-13/CIT02g-18, respectively. Through marker analysis using 30 chromosome 2G-specific markers located in the Pm6 region, the identified recombinants were divided into 14 haplotypes. Pm resistance evaluation of these haplotypes enabled us to narrow Pm6 to a 0.9 Mb physical region of 2BL, flanked by markers CIT02g-20 and CIT02g-18. Six wheat varieties containing Pm6 were identified from a natural population, and they showed increased Pm resistance. This implied Pm6 is still effective, especially when used in combination with other Pm resistance genes.
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Acknowledgements
This research was supported by the Grants from the National Key Research and Development Program (2016YFD0101004, 2016YFD0102001-004), the National Natural Science Foundation of China (Nos. 31571653, 31771782, 31201204), the International Cooperation and Exchange of the National Natural Science Foundation of China (No. 31661143005), the Special Fund of Jiangsu Province for the Transformation of Scientific and Technological Achievements (BA2017138), the Creation of Major New Agricultural Varieties in Jiangsu Province (PZCZ201706), the SAAS Program for Excellent Research Team, the Science and Technology Service Programs of the Chinese Academy of Sciences (KFJ-STS-ZDTP-002), the Jiangsu Agricultural Technology System (JATS) (No. 2019429) and the Key Research and Development Major Project of Ningxia Hui Autonomous Region (No. 2019BBF02022-04).
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WXE and WHY designed the experimental plan. WWT, LML, TX, CAK and WMX performed the experiments. WWT, LML, TX, LYB and WMX managed the materials in the field. WWT, XJ and WXE wrote the manuscript. All authors have read and approved the final manuscript.
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Supplementary Fig.
1 The distribution of previously reported markers forPm6by Qin et al. (2011) along the wheat 2B chromosome. The black boxes indicate introgression fragments from 2G of T. timopheevii; the red arrows point to the recombination site in IGV1-466/Prins and IGV1-465/Prins F2 population. Supplementary Fig. 2: The microcolinearity analysis ofPm6region in Tritium crop. Ta, Td, At, Tu and Hv represent common wheat, Triticum dicoccoides, Aegilops tauschii, Triticum urartu and Hordeum vulgare, respectively. The lines link the homologous genes between chromosomes. Supplementary Fig. 3: The flanking marker ofPm6amplified in a natural group contains 387 materials. Recombinant represents a recombinant derived from IGV1-465/CSph1b which obtains the gene of Pm6. Lanes 1 to 6 are six wheat varieties harboring Pm6; lanes 7 to 21 are representatives without Pm6. Supplementary Fig. 4: Identification ofPm6in a natural population and validation of its contribution toPmresistance. A: Comparison of Pm resistance between wheat varieties with and without Pm6, tested with Blumeria graminis f. sp. tritici (Bgt) isolate E26 and Bgt mixture, respectively; B: the geographical distribution of Pm6-containing wheat varieties in China. (PDF 520 kb)
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Wan, W., Xiao, J., Li, M. et al. Fine mapping of wheat powdery mildew resistance gene Pm6 using 2B/2G homoeologous recombinants induced by the ph1b mutant. Theor Appl Genet 133, 1265–1275 (2020). https://doi.org/10.1007/s00122-020-03546-8
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DOI: https://doi.org/10.1007/s00122-020-03546-8