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Development and characterization of wheat lines carrying stem rust resistance gene Sr43 derived from Thinopyrum ponticum

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Wheat lines carrying Ug99-effective stem rust resistance gene Sr43 on shortened alien chromosome segments were produced using chromosome engineering, and molecular markers linked to Sr43 were identified for marker-assisted selection.

Abstract

Stem rust resistance gene Sr43, transferred into common wheat (Triticum aestivum) from Thinopyrum ponticum, is an effective gene against stem rust Ug99 races. However, this gene has not been used in wheat breeding because it is located on a large Th. ponticum 7el2 chromosome segment, which also harbors genes for undesirable traits. The objective of this study was to eliminate excessive Th. ponticum chromatin surrounding Sr43 to make it usable in wheat breeding. The two original translocation lines KS10-2 and KS24-1 carrying Sr43 were first analyzed using simple sequence repeat (SSR) markers and florescent genomic in situ hybridization. Six SSR markers located on wheat chromosome arm 7DL were identified to be associated with the Th. ponticum chromatin in KS10-2 and KS24-1. The results confirmed that KS24-1 is a 7DS·7el2L Robertsonian translocation as previously reported. However, KS10-2, which was previously designated as a 7el2S·7el2L-7DL translocation, was identified as a 7DS-7el2S·7el2L translocation. To reduce the Th. ponticum chromatin carrying Sr43, a BC2F1 population (Chinese Spring//Chinese Spring ph1bph1b*2/KS10-2) containing ph1b-induced homoeologous recombinants was developed, tested with stem rust, and genotyped with the six SSR markers identified above. Two new wheat lines (RWG33 and RWG34) carrying Sr43 on shortened alien chromosome segments (about 17.5 and 13.7 % of the translocation chromosomes, respectively) were obtained, and two molecular markers linked to Sr43 in these lines were identified. The new wheat lines with Sr43 and the closely linked markers provide new resources for improving resistance to Ug99 and other races of stem rust in wheat.

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Acknowledgments

We thank Dr. G. Francois Marais and Dr. Lili Qi for critically reviewing the manuscript. The authors also thank Mary Osenga, Rachel McArthur, Danielle Holmes, and Xiaohong Jiang for technical support. This research was supported in part by funds to S. S. X. provided through a grant from the Bill & Melinda Gates Foundation to Cornell University for the Borlaug Global Rust Initiative (BGRI) Durable Rust Resistance in Wheat (DRRW) Project and the USDA-ARS CRIS Project No. 5442-22000-037-00D. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.

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The experiments were performed in compliance with the current laws of the USA.

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Authors and Affiliations

  1. Northern Crop Science Laboratory, Cereal Crops Research Unit, USDA-ARS, 1605 Albrecht Blvd. North, Fargo, ND, 58102-2765, USA

    Z. Niu, D. L. Klindworth, T. L Friesen, S. Chao, J.-B. Ohm & Steven S. Xu

  2. Departments of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA

    G. Yu & J. B. Rasmussen

  3. USDA-ARS Cereal Disease Laboratory, St. Paul, MN, 55108, USA

    Y. Jin

  4. Departments of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA

    X. Cai

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  1. Z. Niu
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  2. D. L. Klindworth
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  3. G. Yu
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Correspondence to Steven S. Xu.

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Communicated by H.-C. Jing.

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122_2014_2272_MOESM1_ESM.jpg

Fig. S1. Electropherograms showing the size of the PCR products of six SSR markers on the long arm of chromosome 7D (Xbarc172, Xwmc150, Xbarc121, Xwmc797, Xbarc111, and Xcfa2040) amplified from KS10-2, KS24-1, Thatcher, Chinese Spring (CS), and three CS nullisomic-tetrasomic (NT) lines. The NT lines N7AT7D, N7BT7D, and N7DT7B represent nullisomic for 7A and tetrasomic for 7D, nullisomic for 7B and tetrasomic for 7D, and nullisomic for 7D and tetrasomic for 7B, respectively. Fragment sizes include a 20-bp M13 primer tail. The peak represents the PCR products, whereas the scale on the horizontal and vertical axis represent fragment sizes in base pair (bp) and fluorescent signal intensity, respectively. (JPEG 3667 kb)

122_2014_2272_MOESM2_ESM.jpg

Fig. S2. Validation of SSR marker Xcfa2040 and STS marker Xrwgs30 using common wheat varieties. 1) Jimai 22, 2) Shanrong 1, 3) Jinan 17, 4) Jinan 177, 5) Zhengmai 9023, 6) Amidon, 7) Howard, 8) Alsen, 9) Grandin, 10) Glenn, 11) Faller, 12) Glupro, 13) Ernest, 14) Steele-ND, 15) Reeder, 16) Mott, 17) Kulm, 18) Parshall, 19) Granger, 20) Brick, 21) Russ, 22) Briggs, 23) Traverse, 24) Sabin, 25) Oklee, 26) Ulen, 27) Ada, 28) Tom, 29) Newton, 30) IL06-14262, 31) Thatcher, 32) Chinese Spring, and 33) KS10-2. (JPEG 391 kb)

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Niu, Z., Klindworth, D.L., Yu, G. et al. Development and characterization of wheat lines carrying stem rust resistance gene Sr43 derived from Thinopyrum ponticum . Theor Appl Genet 127, 969–980 (2014). https://doi.org/10.1007/s00122-014-2272-4

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